Microbial networking in cancer: when two toxins collide

Abstract

A recent study by Dejea et al. has demonstrated that two enterotoxigenic bacteria frequently associated with sporadic colorectal cancer, Bacteroides fragilis and pks+ Escherichia coli, are found together in biofilms on tissue from patients with familial adenomatous polyposis. In preclinical mouse models, these two bacteria and their corresponding toxins work synergistically to promote colon cancer.

Main

Bacteria colonise virtually all human body surfaces, and their immunomodulatory and metabolic activities are thought to profoundly affect health. Among the various ecological niches within the body, the gastrointestinal tract is home to the most complex microbial ecosystem with the greatest abundance of microorganisms. The intestinal microbiota can be found as luminal planktonic organisms or associated with the mucosal epithelium, sometimes forming biofilm structures. Biofilms formed of polymicrobial communities have been implicated in human diseases such as medical implant infections, cystic fibrosis pneumonia and periodontitis,1 and a new study suggests that polymicrobial biofilms may also have a role in hereditary colon cancer.2

A number of studies have demonstrated compositional alterations in the luminal or tissue-associated bacteria between healthy and colorectal cancer (CRC) patients.3 Among the various microorganisms contributing to the differences in bacterial composition, Enterobacteriaceae family members that carry the genotoxic pks gene island, enterotoxigenic Bacteroides fragilis (ETBF) with the B. fragilis toxin (bft) gene, and Fusobacterium nucleatum have often been reported.4 However, the potential interactions between biofilm structures and cancer have attracted less attention. In 2014, a study reported that biofilms were associated with 89% of right-sided/proximal CRCs versus 13% of left-sided/distal CRCs, suggesting a correlation between biofilm organisation and carcinogenesis,5 although it is still unclear why biofilms appear to be more strongly associated with proximally located sporadic CRCs. In that study, and a follow-up with additional patients, the biofilm structures were characterised by the presence of Enterobacteriaceae, Bacteroidetes and Fusobacterium among others.5,6 These findings suggested that interactions between bacteria within biofilms may contribute to the development of CRC.

In support of this idea, the recent study by Dejea et al.2 has greatly advanced our understanding of the impact of bacterial interactions in CRC, by showing a synergistic carcinogenic effect in mice between the Enterobacteriaceae pks+ Escherichia coli and ETBF. The authors set out to investigate microbial factors that may contribute to disease onset in patients with a hereditary form of CRC; familial adenomatous polyposis (FAP). Using fluorescence in situ hybridisation to detect bacteria, the authors examined biofilm presence in surgical resections from five patients with FAP. Similar to the biofilms identified from patients with sporadic CRC5, the FAP patient biofilms were polymicrobial, invaded the colon mucus and were found on both polyp and normal mucosal tissues. However, in contrast to biofilms from patients with sporadic CRC, FAP patient biofilms were found throughout the colon (as opposed to primarily in the proximal colon), and were mainly composed of E. coli and B. fragilis. Previous studies have indicated that colibactin, which is produced from the pks gene island, and bft toxins both promote CRC in animal models.7,8 Therefore, the authors investigated the presence of these two genes in cultured bacteria obtained from the mucosal tissues of 25 FAP and 23 normal patients.  PCR analysis revealed an increased prevalence of pks and bft toxin genes in the bacteria from FAP patients versus control patients.

The co-occurrence of ETBF and pks+ E. coli was also confirmed within the biofilms from FAP patients, using laser capture microdissection. To examine the combined impact of both bacteria on disease onset, the authors co-colonised two mouse models with ETBF and pks+ E. coli: ApcMinΔ716/+ mice (to model FAP) and azoxymethane (AOM)-injected wildtype (WT) mice. Co-colonised AOM/WT mice developed more tumours than AOM/WT mice colonised by either bacterium alone; a phenotype that depended on the presence of both pks and bft. The co-colonised ApcMinΔ716/+ mice had a shortened survival time versus the mono-colonised mice (8 weeks versus 15 weeks), although overall tumour numbers were similar between co- and ETBF-colonised mice.

Fecal IgA against specific bacteria have been shown to associate with intestinal pathologies such as inflammatory bowel disease.9 Interestingly, Dejea et al. found co-colonised AOM/WT mice displayed an increased fecal IgA response to E. coli compared with mono-colonised mice, which correlated with increased pks+ E. coli colonisation of the distal colon mucosal tissue. These pieces of evidence suggest that the enhanced tumourigenesis upon co-colonisation related to a shift in the colonisation location of pks+ E. coli from the lumen to the mucosal surface, due to the subsequent increase in E. coli-specific IgA. In vitro experiments with mucin-producing intestinal epithelial cell monolayers demonstrated that colonisation with ETBF or ETBF/pks+ E. coli reduced the mucus layer depth to a comparable amount as the mucin-degrader Akkermansia muciniphila, thus suggesting that the mucin-degrading ability of ETBF may facilitate E. coli relocalisation to the mucosal tissue, by reducing the hindrance caused by the mucus. To test the interplay between mucin-degrading bacteria and genotoxic pks+ E. coli, the authors co-colonised AOM/WT mice with A. muciniphila and pks+ E. coli. Surprisingly, the presence of A. muciniphila reduced tumourigenesis compared with pks+ E. coli colonisation, highlighting the key role of ETBF in carcinogenesis and demonstrating that altering the mucosal mucin integrity is not sufficient to promote the carcinogenic potential of pks+ E. coli. These results indicate that additional contributing factors, such as bft, are required for carcinogenesis. Overall, Dejea et al. demonstrated a synergistic interaction between two carcinogenic bacteria, and established the concept of microbial networks in carcinogenesis.

The authors’ findings suggest that the spatial distribution of bacteria within the colon, including their proximity to the host mucosal barrier as well as interactions with each other, should be investigated further in the context of CRC. Intestinal lumen and even oral microbial composition has previously distinguished healthy subjects from patients with CRC10; however, these studies were conducted to identify biomarkers, and did not address causation. The evidence that ETBF and E. coli are found within biofilms and mucosal tissue from patients with FAP, and have a combined synergistic effect in animal models of CRC (Fig. 1), suggest that screening for the presence of these two bacterial strains may help assess cancer risk in humans.

Fig. 1
figure1

ETBF and pks+ E. coli are found together within FAP patients and synergise to promote colorectal cancer. a Increased prevalence of ETBF carrying Bacteroides fragilis toxin and E. coli carrying pks+ toxin, in polyps and macroscopically normal biofilm tissues from FAP patients. b Wildtype mice treated with azoxymethane and co-colonised with ETBF and pks+E. coli developed more tumours than mono-colonised mice. ETBF, through mucin degradation, favours pks+E. coli mucosal tissue colonisation and facilitate pks+ -induced genotoxicity. AOM, azoxymethane; FAP, familial adenomatous polyposis; ETBF, enterotoxigenic Bacteroides fragilis

The events leading to increased microbial co-occurrence and development of CRC are unclear. One contributing factor appears to be host genetics, as FAP is driven by mutations in the tumour-suppressing adenomatous polyposis coli gene, and ApcMin/+ mouse models have increased Bacteroides and Enterobacteriaceae compared with WT mice.2 B. fragilis and Enterobacteriaceae members have also been identified within mucosal biofilms of intestinal biopsies collected from patients with inflammatory bowel disease,11 suggesting intestinal inflammation also influences host susceptibility. In mice, increased mucus penetrability and proximity of bacteria to the mucosal layer are side effects of both high-fat12 and western-style13 diets, which are both carcinogenic risk factors. Whether diets have similar effects within hereditary CRC patients, by predisposing them to bacterial colonisation of the mucosal surface, is unclear. Interestingly, administering the dietary fibre inulin was shown to ameliorate the negative impact that high-fat or western style diets have on the mucus barrier and bacteria localisation in mice.12,13 This may indicate a potential preventative approach.

The polymicrobial nature of CRC suggests additional microbial networking likely exists within the intestine, which could positively or negatively influence carcinogenesis outcomes. It would be important to characterise these microbial networks and identify specific nodes that could represent preventive or therapeutic targets. Dejea et al. provide invaluable information on the functional interaction between two carcinogenic microorganisms, and the study paves the way for future studies elucidating microbial networks in cancer.

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Acknowledgements

This research was supported by National Institutes of Health grants R01 DK73338 and from the University of Florida, Department of Medicine Gatorade Fund to C. Jobin.

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Tomkovich, S., Jobin, C. Microbial networking in cancer: when two toxins collide. Br J Cancer 118, 1407–1409 (2018). https://doi.org/10.1038/s41416-018-0101-2

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