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Allogeneic transplantation of peripheral blood stem cell grafts results in a massive decrease of primitive hematopoietic progenitor frequencies in reconstituted bone marrows

Bone Marrow Transplantation volume 55, pages 100–109 (2020)Cite this article

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Abstract

The success of allogeneic hematopoietic stem cell transplantation (alloSCT) is indicated by the reconstitution of the peripheral blood system of patients after alloSCT and the engraftment of hematopoietic stem and progenitor cells (HSPCs) into their bone marrow (BM). The number of CD34+ cells is commonly used as surrogate for the content of hematopoietic stem cells in the grafts. During the last decade, several antigens (including CD133, CD45RA, CD38, and CD10) were identified allowing discrimination of different HSPC subpopulations within the human CD34+ cell compartment. Although such studies increased our understanding of early human hematopoiesis tremendously, hardly any study dissected the CD34+ compartment in the alloSCT setting. Consequently, we comprehensively analyzed the CD34+ compartment in G-CSF-stimulated peripheral blood stem cell grafts of allogeneic donors, in BM samples of the respective recipients 4 weeks after alloSCT, and in BM samples of healthy donors. We demonstrate that alloSCT is associated with a dramatic shift from primitive to more mature HSPC types. Upon investigating whether the composition of engrafted CD34+ cells has any impact on the incidence and severity of graft-versus-host disease, we did not find any correlation. However, more detailed analyses of the CD34+ compartment may elucidate associations with other transplantation-related complications.

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Acknowledgements

We are grateful to Iina Fischer from the Deutsches Rotes Kreuz for providing bone marrow samples of five Westdeutsche SpenderZentrale (WSZE) donors as healthy controls and to Martina Franke for performing the flow cytometry analyses. André Görgens is an International Society for Advancement of Cytometry (ISAC) Marylou Ingram Scholar (2019–2023).

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Authors and Affiliations

  1. Department of Bone Marrow Transplantation, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

    Lambros Kordelas & Dietrich W. Beelen

  2. Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

    André Görgens, Stefan Radtke, Peter A. Horn & Bernd Giebel

  3. Clinical Research Center, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    André Görgens

  4. Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    Stefan Radtke

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  1. Lambros Kordelas
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Correspondence to Lambros Kordelas or Bernd Giebel.

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Kordelas, L., Görgens, A., Radtke, S. et al. Allogeneic transplantation of peripheral blood stem cell grafts results in a massive decrease of primitive hematopoietic progenitor frequencies in reconstituted bone marrows. Bone Marrow Transplant 55, 100–109 (2020). https://doi.org/10.1038/s41409-019-0645-7

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