Dear Editor,
The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) poses a great threat to the public health of people and the normal economic and social development around the world. As of January 8, 2021, more than 88 million people were infected with SARS-CoV-2, resulting in more than 1.9 million death. Early detection and treatment of SARS-CoV-2 are of prime significance for effective control of COVID-19. Currently, nucleic acid amplification (e.g., RT-PCR) and detection of IgG, IgM, or viral antigen are employed in clinical diagnosis1, and there is only one drug, remdesivir, was approved for COVID-19 treatment. Although various virus-based and host-based therapeutics, such as remdesivir, lopinavir/ritonavir, umifenovir, ribavirin, chloroquine, hydroxychloroquine, interferon, Tocilizumab, convalescent plasma, neutralizing antibody, and traditional Chinese medicine, have been tested to treat COVID-192, developing novel therapeutic are urgently required. Nucleic acid modalities including aptamer oligonucleotide constitute a promising next-generation solution for disease diagnosis and treatment3,4,5. Developing an aptamer-based theranostic regimen represents a potentially effective way to control COVID-19.
We herein report that six novel DNA aptamers, screened via capillary electrophoresis (CE)-based systematic evolution of ligands by exponential enrichment (SELEX) method, can specifically recognize SARS-CoV-2 in human serum, potently inhibit SARS-CoV-2 infection by blocking the receptor-binding domain (RBD) domain of spike (S) protein subunit 1. The spike protein composed of S1 and S2 subunits, mediating the recognition and infection of SARS-CoV-2. Particularly, the S1 subunit-containing RBD is an important site for host neutralizing antibodies and a promising target for vaccine design. It can interact with the angiotensin-converting enzyme (ACE2) to infect human respiratory epithelial cells. Hence, we screened S1 protein against DNA aptamers, aiming to achieve specific detection and/or effective inhibition of SARS-CoV-2 infection.
SELEX of oligonucleotide aptamers of active virulent viruses has extremely high safety requirements for separation equipment and environment. Therefore, in order to increase the feasibility of screening strategies, recombinant S1 protein was thrown to capture the entire SARS-CoV-2. In order to ensure the evolution efficiency of the affinity and specificity of aptamer candidates, we adopted a combined SELEX strategy of the positive, negative, and complex background screening process, and continuously reduced the concentration of S1 to increase the screening pressure to obtain high-performance aptamers (Supplementary Fig. S1). In the final round of screening, the evolved sub-library bound to S1 protein to form a stable complex in human serum. After high-throughput sequencing, the six most enriched aptamer candidates (nCoV-S1-Apts) were selected for performance verification (Supplementary Table S1).
The interaction analysis results of capillary zone electrophoresis (CZE) showed that due to the different binding ratios, nCoV-S1-Apts formed stable polymorphic complexes with S1 (Fig. 1a), and had nanomolar affinity (KD = 0.118 ± 0.033–85.610 ± 14.219 nM) (Fig. 1b). The interfering substances existing in vivo environments including human serum albumin (HSA), IgG Fc, normal human serum (NHS), and IgG Anti-S1 were introduced to evaluate the specificity of nCoV-S1-Apts. The results showed that the peak area of nCoV-S1-Apt1 did not change significantly during the interaction with the above interfering components (Fig. 1c and Supplementary Fig. S2). The results indicated that nCoV-S1-Apts exhibited stronger specificity and better anti-plasma protein interference properties, which is beneficial to reduce potential off-target effects and cytotoxicity (Supplementary Fig. S3). The affinity and specificity of nCoV-S1-Apt1 were verified by surface plasmon resonance (SPR) (Fig. 1d and Supplementary Fig. S4).
In order to determine the possible binding site(s) of nCoV-S1-Apt1, we docked nCoV-S1-Apt1 to S1. Visualization of docking possess was performed using PyMOL. The results showed that nCoV-S1-Apt1 residues from 45 to 71 bind to the two main active sites designated for ACE2 binding (Supplementary Fig. S5). Different types of interactions were observed in the S1-aptamer complex, e.g., 32 polar contacts, 20 VdW, 15 H-bonds, 26 hydrophobic, 4 ionic, and 18 aromatic, yielding −12.17 kcal/mol binding free energy with KD value of 0.1 nM. This data suggested that nCoV-S1-Apt1 may be used as a potent inhibitor of the RBD domain of S1.
In addition, the binding affinities between nCoV-S1-Apt1 and RBD were determined via CE (Fig. 1e). Data showed that nCoV-S1-Apt1 combined with RBD to form stable complexes with a KD of 1.56 ± 0.22 μM. Therefore, we sought to determine the binding kinetics of nCoV-S1-Apts and S1. The KD of nCoV-S1-Apts to S1 is 1.5 × 104 times lower than that of RBD (Supplementary Fig. S6), which indicates that the binding time of nCoV-S1-Apts to S1 is significantly longer than that of RBD. S1 provides a more stable binding site for the aptamer than RBD, thereby greatly extending the binding time and exhibiting the kinetic advantage of drug-receptor interaction, and further helping to significantly improve the interaction potency.
To evaluate the recognition and detection potential of nCoV-S1-Apt1, we developed an AuNPs colorimetric assay, which could quickly and specifically detect S1 protein in human serum with a detection limit of ~3.125 nM (Fig. 1f, g and Supplementary Fig. S7). Meanwhile, FAM-labeled nCoV-S1-Apt1 was employed as a fluorescent probe to capture S1 protein in human serum (Fig. 1h). The CE laser-induced fluorescence (LIF) analysis results showed that nCoV-S1-Apt1 formed a significant complex with 5 nM of S1 protein in human serum. In addition, we introduced the SARS-CoV-2 spike-pseudotyped virus into the human serum, and tested it on CE-LIF. The results demonstrated that FAM-nCoV-S1-Apt1 exhibited satisfactory recognition (Fig. 1i) and detection linearity (Fig. 1j) in the range of 102–105 virus particles.
To confirm the multi-scale inhibitory effect of nCoV-S1-Apt1 on SARS-CoV-2, inhibition tests were carried out from 3 levels of RBD, S1, and pseudovirus. We established a ternary competitive binding assay, in which ACE2 protein or anti-S1 IgG was introduced to compete with the nCoV-S1-Apt1 (Fig. 1k). The results showed that the addition of ACE2 protein or anti-S1 IgG reduced the nCoV-S1-Apt1/S1 complex, suggesting that nCoV-S1-Apt1 competed with ACE2 protein or anti-S1 IgG to bind to S1 at a specific site. Furthermore, we evaluated the IC50 of nCoV-S1-Apt1 on the binding of RBD to ACE2 by competitive ELISA (Fig. 1l). nCoV-S1-Apt1 showed potent inhibition activity with IC50 of 80.12 nM.
To identify and verify the inhibitory effect and neutralization performance of nCoV-S1-Apt1 on S1/ACE2 binding and SARS-CoV-2 infection, we next employed a SARS-CoV-2 spike-pseudovirus infection model, which stably expresses both green fluorescent protein (GFP) and firefly luciferase. The data manifested that the introduction of nCoV-S1-Apt1 significantly reduced the expression of GFP and luciferase (Fig. 1m, n and Supplementary Fig. S8), and nCoV-S1-Apt1 displayed a dose-dependent inhibitory profile on pseudovirus infection. Therefore, nCoV-S1-Apt1 can prevent SARS-CoV-2 infection by binding to the RBD of S1 and hindering the recognition and interaction of S1 to ACE2, which implies its great potential as a new neutralizing antiviral agent against SARS-CoV-2 infection.
In summary, six DNA aptamers with high affinity to S1 protein were screened by CE-based SELEX in this study, and nCoV-S1-Apt1 showed excellent neutralization activity. Hence, this study proposes the potential use of aptamer in COVID-19 therapy or prevention, and provides a basis for the design of fusion inhibitor, neutralizing oligonucleotide, or targeted delivery system for SARS-CoV-2. It also presents an universal and feasible identification strategy for virus-suppressing aptamer selection based on multi-scale target selection. In addition, an authentic SARS-CoV-2 virus challenge and in vivo verification test should be performed in the next stage. We are working on it and digging in establishing an aptamer-based diagnosis and treatment platform for control of COVID-19.
References
Ravi, N., Cortade, D. L., Ng, E. & Wang, S. X. Diagnostics for SARS-CoV-2 detection: a comprehensive review of the FDA-EUA COVID-19 testing landscape. Biosens. Bioelectron. 165, 112454 (2020).
Song, Y. et al. COVID-19 treatment: close to a cure? A rapid review of pharmacotherapies for the novel coronavirus (SARS-CoV-2). Int J. Antimicrob. Agents 56, 106080 (2020).
Zhao, Y. et al. Nucleic acids analysis. Sci. China Chem. 64, 171–203 (2020).
Hu, B. et al. Therapeutic siRNA: state of the art. Signal Transduct. Target Ther. 5, 101 (2020).
Song, Y. et al. Discovery of aptamers targeting the receptor-binding domain of the SARS-CoV-2 spike glycoprotein. Anal. Chem. 92, 9895–9900 (2020).
Acknowledgements
This work was supported by the National Natural Science Foundation of China (21675012, 21874010, 21827810, 31871003, and 31901053), the Beijing Nova Program from Beijing Municipal Science & Technology Commission (Z201100006820005), the Beijing-Tianjin-Hebei Basic Research Cooperation Project (19JCZDJC64100), the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology (2020-2022), the Fundamental Research Funds for the Central Universities from Beijing Institute of Technology (2018CX01023), the Hunan Provincial Natural Science Foundation of China (2018JJ1019), the Hu-Xiang Young Talent Program (2018RS3094), the Natural Science Foundation of Guangdong Province (2019A1515010776), the Postdoctoral Science Foundation of China (2020M670169), and Guangxi Key Research and Development Project (No. AB20117001). We thank Biological & Medical Engineering Core Facilities (Beijing Institute of Technology) for providing advanced equipment.
Author information
Authors and Affiliations
Contributions
Y.H. and F.Q. conceived and designed the experiments. G.Y. performed the majority of the experiments. Z.L., I.M., and L.Z. participated in experiments. W.G. involved in pseudovirus assays. Y.H., F.Q., and G.Y. analyzed the data. W.W., H.X., and Y.Z. provided insightful suggestions. G.Y. and Y.H. wrote the paper. All authors read and approved the contents of the manuscript.
Corresponding author
Ethics declarations
Competing interests
F.Q., Y.H., and G.Y. have filed a patent related to the aptamers. The remaining authors declare no competing interests.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Yang, G., Li, Z., Mohammed, I. et al. Identification of SARS-CoV-2-against aptamer with high neutralization activity by blocking the RBD domain of spike protein 1. Sig Transduct Target Ther 6, 227 (2021). https://doi.org/10.1038/s41392-021-00649-6
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41392-021-00649-6
This article is cited by
-
Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity
Signal Transduction and Targeted Therapy (2024)
-
A new DNA aptamer which binds to SARS-CoV-2 spike protein and reduces pro-inflammatory response
Scientific Reports (2024)
-
Comparative analysis of aptamers binding to SARS-CoV-2 N protein using capillary electrophoresis and bio-layer interferometry
Analytical and Bioanalytical Chemistry (2024)
-
Nanobiosensors: A powerful Technology for Early Detection of Plant Parasitic Nematodes
Sensing and Imaging (2024)
-
Inhibition of SARS-CoV-2 infection in human airway epithelium with a xeno-nucleic acid aptamer
Respiratory Research (2023)