The use of gene expression as a disease stratification tool of neonatal encephalopathy

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Fig. 1: Expression of the six selected genes analyzed by LMM in controls and NE patients during the first 11 h of life.


  1. 1.

    Balada, R. et al. Enquiring beneath the surface: can a gene expression assay shed light into the heterogeneity among newborns with neonatal encephalopathy? Pediatr. Res. (2020).

  2. 2.

    Ioannidis, J. P. et al. Repeatability of published microarray gene expression analyses. Nat. Genet. 41, 149–155 (2009).

    CAS  Article  Google Scholar 

  3. 3.

    Montaldo, P. et al. Whole blood gene expression reveals specific transcriptome changes in neonatal encephalopathy. Neonatology 115, 68–76 (2019).

    CAS  Article  Google Scholar 

  4. 4.

    Montaldo, P., Lally, P. J., Oliveira, V. & Thayyil, S. Hypothermic neuroprotection for neonatal encephalopathy in low- and middle-income countries: a new approach to an old problem. NeoReviews 19, e735–e741 (2018).

    Article  Google Scholar 

  5. 5.

    Montaldo, P. et al. Transcriptomic profile of adverse neurodevelopmental outcomes after neonatal encephalopathy. Nat. Scientific Rep. (2020) (in press).

  6. 6.

    Peng, R. D. Reproducible research in computational science. Science (New York, N.Y.) 334, 1226–1227 (2011).

    CAS  Article  Google Scholar 

  7. 7.

    Kok, M. G. M. et al. Small sample sizes in high-throughput miRNA screens: a common pitfall for the identification of miRNA biomarkers. Biomol. Detect. Quantif. 15, 1–5 (2017).

    Article  Google Scholar 

  8. 8.

    Pritchard, C. C., Cheng, H. H. & Tewari, M. MicroRNA profiling: approaches and considerations. Nat. Rev. Genet. 13, 358–369 (2012).

    CAS  Article  Google Scholar 

  9. 9.

    Dypås, L. B., Gützkow, K. B., Olsen, A.-K. & Duale, N. MiRNA profiles in blood plasma from mother–child duos in human biobanks and the implication of sample quality: circulating miRNAs as potential early markers of child health. PLoS ONE 15, e0231040-e (2020).

    Article  Google Scholar 

Download references

Author information




P.M. and S.T. conceptualized and designed the study. P.M. and C.B. collected the data. P.M. and C.B. analyzed the data. C.B. and S.T. drafted the initial manuscript. P.M. supervised the study. S.T. and P.M. reviewed and revised the manuscript critically. All authors approved the final manuscript as submitted.

Corresponding author

Correspondence to Paolo Montaldo.

Ethics declarations

Competing interests

The authors declare no competing interests.

Patient consent

All the parents of the neonates who were enrolled in the study provided their written informed consent.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Burgod, C., Thayyil, S. & Montaldo, P. The use of gene expression as a disease stratification tool of neonatal encephalopathy. Pediatr Res (2020).

Download citation