Community-acquired pneumonia (CAP) is the leading cause of child deaths around the world. Recently, the vitamin D receptor (VDR) gene has emerged as a susceptibility gene for CAP.
To evaluate the association of the VDR gene Fok I polymorphism with susceptibility to CAP in Egyptian children.
This was a multicenter case-control study of 300 patients diagnosed with CAP, and 300 well-matched healthy control children. The VDR Fok I (rs2228570) polymorphism was genotyped by PCR-restriction fragment length polymorphism (RFLP), meanwhile serum 25-hydroxy vitamin D (25D) level was assessed using ELISA method.
The frequencies of the VDR FF genotype and F allele were more common in patients with CAP than in our control group (OR = 3.6; (95% CI: 1.9–6.7) for the FF genotype; P = 0.001) and (OR: 1.8; (95% CI: 1.4–2.3) for the F allele; P = 0.01). Patients carrying the VDR FF genotype had lower serum (25D) level (mean; 14.8 ± 3.6 ng/ml) than Ff genotype (20.6 ± 4.5 ng/ml) and the ff genotype (24.5 ± 3.7 ng/ml); P < 0.01.
The VDR gene Fok I (rs2228570) polymorphism confers susceptibility to CAP in Egyptian children.
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We thank the staff of Pediatric Pulmonology and Outpatient Clinics in Zagazig University, Ain-Shams and Cairo University hospitals for their collaboration in sampling as well as our patients who participated in the study.
H.A.Z. submitted the manuscript. M.A.A. designed the study. A.M.K. collected clinical data and coordinated the sample collection (Zagazig University). N.M.A. collected clinical data and coordinated the sample collection (Ain-Shams University). M.M.S. collected clinical data and coordinated the sample collection (Cairo University). M.S.H. and H.A.A.E. performed the statistical analysis. M.A.N. and A.A.S. helped to draft the manuscript. A.M.S., A.A.M., and M.E.H. wrote the manuscript. A.A.A., M.T.Z., and S.S.A.E. critically revised the final version. A.M.A., R.M.N., and G.M.A. performed laboratory analysis and genotyping. All authors read and approved all the manuscript.
The authors declare no competing interests.
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