Abstract
At the molecular level, epithelial-to-mesenchymal transition (EMT) necessitates extensive transcriptional reprogramming which is orchestrated by a small group of gene-regulatory factors that include the zinc-finger DNA-binding protein SNAIL1. Although SNAIL1 is a well-known master regulator of EMT, knowledge of its immediate target genes is incomplete. Here, we used ChIP-seq to identify genes directly regulated by SNAIL1 in colorectal adenocarcinoma cells. When comparing the genomic distribution of SNAIL1 to that of the intestinal stem cell (ISC) transcription factors ASCL2 and TCF7L2, we observed a significant overlap. Furthermore, SNAIL1 ChIP-seq peaks are associated with a substantial fraction of ISC signature genes. In two colorectal cancer cell lines, we verified that SNAIL1 decreases ISC marker expression. Likewise, SNAIL1 directly represses the proto-oncogene MYB, and the long noncoding RNA (lncRNA) WiNTRLINC1, a recently described regulator of ASCL2. SNAIL1 targets multiple regulatory elements at the MYB and WiNTRLINC1 loci, and displaces ASCL2 and TCF7L2 from their binding regions at a MYB downstream regulatory element. Correlation analyses and expression profiling showed antiparallel expression of SNAIL1 and MYB in colorectal and breast cancer cell lines and tumor transcriptomes, suggesting that SNAIL1 controls MYB expression in different tissues. MYB loss-of-function attenuated proliferation and impaired clonogenicity in two- and three-dimensional cell cultures. Therefore, SNAIL1-mediated downregulation of MYB and ISC markers like WiNTRLINC1 likely contributes to the decrease in proliferation known to be associated with EMT, while simultaneously abrogating stemness features of colorectal cancer cells. Apparently, the relationship between EMT and stemness varies in different tumor entities.
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Introduction
Epithelial–mesenchymal transitions (EMT) are thought to facilitate several steps of the invasion–metastasis cascade [1,2,3]. Phenotypically, cells undergoing EMT lose their apical–basal polarity, tight intercellular contacts, and interactions with the basal membrane. They acquire a spindle-like appearance, gain motility, and invasiveness, and may acquire enhanced tumor-initiation capacities [1,2,3]. These radical changes in cellular traits demand for extensive transcriptional reprogramming, comprising down- and upregulation of epithelial and mesenchymal gene expression programs, respectively. Members of the SNAIL, ZEB and TWIST families of transcription factors are EMT master regulators which trigger these cascades in gene expression changes [1,2,3]. Although EMT-associated transcriptional adaptations are intensely investigated, their full extent and especially the complement of direct target genes of EMT master regulators have yet to be identified.
SNAIL1 proteins are evolutionary conserved zinc-finger transcription factors [4]. They recognize a specific DNA sequence motif 5′-CAGGTG-3′ which represents a variant of the E-box motif [5]. SNAIL1 proteins mainly act as transcriptional repressors, targeting, for instance, CDH1, coding for the cell–cell adhesion protein E-Cadherin, CLDN3, FOXA1, and the invasion suppressor EPHB3 [6,7,8,9]. Notably, SNAIL1 proteins can induce EMT in a variety of tissues, and different EMT programs may exist [10]. Therefore, an interesting question is whether transcriptional programs downstream of SNAIL1 are invariant or cell-type-specific. This could be addressed by comparing SNAIL1-bound cis-regulatory DNA elements and their associated genes in different cellular backgrounds. However, currently this information is available only for breast cancer (BRCA) EMT models [10,11,12].
EMT is commonly thought to enhance stemness of cancer cells [13,14,15,16], but contrasting results were also reported [9, 17, 18]. Further investigations aiming to clarify the relationship between stem cell properties and EMT are therefore needed. Colorectal cancer (CRC) appears well suited for this purpose. There is convincing evidence that the cells-of-origin in CRC are intestinal stem cells (ISCs) [19, 20]. ISCs have an epithelial character and are marked by the expression of a distinctive gene signature [21]. Furthermore, signal transduction pathways and transcription factors with crucial roles in ISC maintenance are known. Examples are the WNT/β-CATENIN pathway and its nuclear effector TCF7L2 [22, 23] which directly control the expression of many ISC signature genes [24,25,26,27,28]. This includes ASCL2 which codes for a basic helix-loop-helix transcription factor and plays an essential role in ISC fate decisions [22, 29]. Moreover, TCF7L2 and ASCL2 frequently co-occupy regulatory DNA elements at ISC signature genes and synergize in their regulation [29]. Altogether, the knowledge about ISC characteristics and their regulators provide excellent opportunities to examine the impact of EMT on stem cell features at a molecular level.
Here, we used chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) to determine the genomic distribution of murine SNAIL1 in colorectal adenocarcinoma cells with pronounced stem/progenitor character [9, 30]. SNAIL1 was found to occupy a significant number of ISC signature genes and to downregulate several of them. Specifically, the intestinal stemness-related genes WiNTRLINC1 and MYB are two newly identified genes which are directly repressed by SNAIL1. Furthermore, SNAIL1-bound regions frequently colocalize with sites occupied by TCF7L2 and ASCL2, and we provide evidence that SNAIL1 antagonizes TCF7L2 and ASCL2. Apparently, SNAIL1-induced EMT impairs stem cell features of CRC cells.
Results
Genome-wide mapping of Snail1-binding regions in CRC cells
To identify genes that are directly regulated by SNAIL1 proteins, we expressed epitope-tagged murine SNAIL1 (Snail1-HA) in LS174T CRC cells from a doxycycline- (Dox-) inducible promoter [9], and performed ChIP-seq from untreated and Dox-treated cells. In two independent biological replicates, we mapped a total of 1501 Snail1-HA ChIP-seq peaks, 661 of which were identified in both replicates (Fig. 1a, Supplementary Table S1). Based on nearest neighbor relationships, Snail1-HA-bound regions were linked to 1307 genes, 627 of which were common to both replicates (Fig. 1a, Supplementary Table S1). Gene set enrichment analysis (GSEA) indicated that Snail1-HA ChIP-seq peak-associated genes were related to differentiation, morphogenesis, organogenesis, signaling, and cell junctions, which agrees well with the known biological functions of SNAIL1 proteins [1, 4] (Supplementary Fig. S1a, Supplementary Table S2).
Overall, 44% of the Snail1-HA-bound regions were found within 1 kb upstream of transcriptional start sites (TSS). An additional 46% were located in intergenic regions and promoter-distal introns (Fig. 1b), indicating that Snail1-HA controls gene expression to a larger degree than commonly assumed from remote regulatory DNA elements. De novo motif deduction revealed the pervasive occurrence of three variants of the cognate SNAIL1 DNA-recognition motif within Snail1-HA ChIP-seq peaks [5]. 822/823 and 1332/1338 Snail1-HA ChIP-seq peaks in replicates 1 and 2, respectively, harbored such motifs considering an alignment score against the position weight matrix higher than 80% (Fig. 1c, Supplementary Fig. S1b). The only other DNA sequence elements enriched were highly G/C-rich (Fig. 1c) which may reflect the high G/C content of promoter regions (Fig. 1c). Genes associated with Snail1-HA ChIP-seq peaks included known SNAIL1 targets [7,8,9, 31], which are downregulated in Snail1-HA-expressing LS174T and HT29 cells (Fig. 1d, Supplementary Fig. S2) [8]. To investigate whether Snail1 regulates these genes when expressed at endogenous levels and to indirectly validate our mapping results in another cell model, we made use of MCF10A cells. These cells undergo SNAIL1-dependent EMT when treated with TGFβ1 [32]. In addition, SNAIL1 ChIP-on-chip data exist for MCF10A cells. We selected several genes, which were bound and regulated by Snail1-HA in LS174T cells, and whose promoter regions showed SNAIL1-occupancy also in MCF10A cells [12]. Indeed, concomitant with an increase in SNAIL1 expression, these genes are downregulated in TGFβ1-treated MCF10A cells (Supplementary Fig. 3), suggesting that SNAIL1 targets these genes not just when overexpressed. Based on these results the mapped ChIP-seq peaks appear to represent a high confidence collection of Snail1-HA binding regions.
To explore the regulatory impact of Snail1-HA occupancy we determined Snail1-HA-induced gene expression changes on a global scale and analyzed transcriptional responses of Snail1-HA-bound genes (Supplementary Fig. 4a, Supplementary Table S3). Overall, 8% of the genes associated with Snail1-HA ChIP-seq peaks were also significantly downregulated 6 h post Snail1-HA induction. The fraction of genes both deregulated and bound by Snail1-HA increased over time, and included a growing number of genes that were upregulated (Supplementary Fig. 4a, Supplementary Table S3). Overall, 44% of all Snail1-HA-binding events eventually translated into gene expression changes. These findings are in agreement with the notion that EMT is a process that gradually evolves [33], and that SNAIL1 proteins act mainly but not exclusively as transcriptional repressors [12, 34,35,36].
SNAIL1 proteins and their relative SLUG (SNAIL2) were implicated in EMT induction in different tissues and tumor entities. To explore to which extent Snail1-HA-binding regions and target genes are cell-type specific, and whether they are shared by SLUG, we compared the genome-wide distribution of Snail1 and Slug in human LS174T CRC cells and murine BRCA cells [10]. Even though we had identified some common targets in LS174T and MCF10A cells, the more comprehensive comparison based on ChIP-seq data showed that the number of Snail1-bound regions and their genic distribution differed considerably in the CRC and BRCA backgrounds (Supplementary Fig. S5, Supplementary Tables S4, S5). The binding pattern of Slug deviated even more, and showed a preponderance of promoter-distal introns and intergenic regions. Consistent with their divergent chromosomal distribution, only few genomic regions were bound by both Snail1 and Slug in human and mouse cells, respectively. We conclude that the chromosomal distribution patterns of Snail1 and Slug are largely cell-type- and factor-specific.
Snail1-HA ChIP-seq peaks colocalize with regions bound by ASCL2 and TCF7L2
To examine the relationship between Snail1-HA and ISC features we asked whether components of the murine ISC gene signature [21] were associated with Snail1-HA ChIP-seq peaks. Upon identifying the corresponding human genes it turned out that 11% of this signature had Snail1-HA ChIP-seq peaks in their vicinity (Fig. 2a, Supplementary Table S6). The regulatory impact of Snail1-HA on selected ISC signature genes was examined by quantitative reverse transcriptase PCR (qRT-PCR), demonstrating their downregulation by Snail1-HA (Supplementary Fig. S2). These results show that Snail1-HA interferes with the expression of stemness-associated genes in CRC cells.
In addition, we compared the genome-wide binding patterns of Snail1-HA and the ISC factors ASCL2 and TCF7L2. We observed a significant colocalization of Snail1-HA, TCF7L2, and ASCL2 ChIP-seq peaks (Fig. 2b, Supplementary Table S7), including 274 regions upon which all three factors converge. Further comparison of patterns of transcription factor occupancy and gene expression changes revealed that 32% (1798/5662) and 31% (2067/6576) of all genes bound by ASCL2 and TCF7L2, respectively, were up- and downregulated in the presence of Snail1-HA (Supplementary Fig. S4b, Supplementary Table S3), most of them, however, apparently in an indirect manner. Interestingly, from a Snail1-HA point-of-view, between 51 and 61% of the genes bound by Snail1-HA and deregulated by Snail1-HA over a time course of 96 h showed co-occupancy by ASCL2. Among these, nearly half were additionally bound by TCF7L2 (Supplementary Fig. S4c, Supplementary Table S8). Only a minority of genes showed dual occupancy by Snail1-HA and TCF7L2. Moreover, even though nearly equal numbers of genes bound by ASCL2 and TCF7L2 were up- and downregulated in the presence of Snail1-HA (Supplementary Fig. S4b), those genes that were co-occupied by Snail1-HA, were predominantly repressed at every time point analyzed (Supplementary Fig. S4c).
The frequent colocalization of ChIP-seq peaks suggested that Snail1-HA might expel ASCL2 and TCF7L2 from their binding sites. To test this, we focused on two cases of coinciding ChIP-seq peaks at gene loci with functional importance for intestinal stemness (Fig. 2c). One is an intergenic element 85 kb downstream of the TSS of the MYB proto-oncogene. Despite its location, this element controls MYB expression in a TCF7L2-dependent manner for several reasons. First, deletion of the +85 kb element by using the CRISPR/Cas9 technology abrogates MYB but not AHI1 expression (Supplementary Fig. S6). Second, MYB, but not the adjacent AHI1 gene is repressed by Snail1-HA (Supplementary Fig. S7a), and third, knockout of TCF7L2 leads to a collapse of the active chromatin structure at the MYB +85 kb element and simultaneously reduces MYB but not AHI1 expression (Supplementary Fig. S7b, c). The second example is WiNTRLINC1 which codes for a long noncoding RNA controlling ASCL2 expression [37]. Expression analysis of WiNTRLINC1 and ASCL2 showed transient and long-lasting repression of both genes upon Snail1-HA induction in LS174T and HT29 cells, respectively (Supplementary Fig. S8b). Consistent with the regulatory interdependency between WiNTRLINC1 and ASCL2 [37] the two genes showed identical expression dynamics. We also verified binding of Snail1-HA at multiple positions around WiNTRLINC1 (Supplementary Fig. S8c) marking WiNTRLINC1 as novel Snail1-HA target. We then examined occupancy of selected ChIP-seq peaks at the WiNTRLINC1 locus and the MYB +85 kb element by Snail1-HA, ASCL2, and TCF7L2. Snail1-HA specifically associated with two ChIP-seq peaks at the WiNTRLINC1 locus, and the MYB +85 kb element, albeit levels of occupancy declined from 6 h to 24 h post induction (Supplementary Fig. S9c, d). In the absence of Snail1-HA, TCF7L2, and ASCL2 occupied all genomic regions examined. Upon Snail1-HA induction, ASCL2 and TCF7L2, which is not deregulated by Snail1-HA (Supplementary Fig. S10), dissociated from the MYB +85 kb element and disappeared almost completely over time. In contrast, at the WiNTRLINC1 locus, ASCL2 and TCF7L2 showed only a tendency to dissociation (Supplementary Fig. S9).
To find out whether Snail1-HA-induced dissociation of ASCL2 involves competition for the same binding sites, we performed electrophoretic mobility shift assays (EMSAs). At the WiNTRLINC1 locus, two of the three identified ASCL2-binding sites were indeed also recognized by Snail1-HA (Supplementary Fig. 11). At the MYB +85 kb region, however, Snail1-HA and ASCL2 interacted with nonidentical DNA elements (Supplementary Fig. 12). Sequence specificity of all interactions was demonstrated by mutagenesis of the 5′-CAGGTG-3′ and 5′-CAGCTG-3′ motifs, which resulted in loss of binding by Snail1-HA and ASCL2, respectively (Fig. 4c, Supplementary Figs. S11, S12). From these observations we conclude that Snail1-HA can displace ASCL2 from cis-regulatory DNA elements by competitive and noncompetitive mechanisms.
Inverse relationship of SNAI1 and MYB expression in colorectal and BRCA
Next, we focused on the regulatory relationship between SNAIL1 and MYB, and the contribution of MYB to stemness-related aspects of CRC cells. Pairwise-correlation analyses of gene expression data from CRC and BRCA samples revealed that MYB expression is positively correlated with that of epithelial marker genes (Fig. 3a, Supplementary Fig. S13a). In contrast, MYB expression is negatively correlated with that of EMT inducers and mesenchymal markers. Moreover, we found a significant survival advantage for patients with higher MYB levels when analyzing the combined TCGA colon and rectal adenocarcinoma cohorts and the pan-cancer cohort (Supplementary Fig. S13b). This, however, was not observed in BRCA samples (Supplementary Fig. S13b).
The inverse relationship between SNAI1 and MYB expression was verified in a panel of CRC cell lines and two BRCA cell lines with epithelial (MCF7) versus mesenchymal (MDA-MB-231) characteristics (Fig. 3b, c) [38]. Consistent with these anticorrelations, overexpression of Snail1-HA in CRC cells resulted in the rapid downregulation of MYB (Fig. 3d, e).
Expression of Snail1-HA reduces active chromatin features at the MYB locus
Aside from the MYB +85 kb element, ChIP-seq had identified two Snail1-HA-bound regions within MYB intron 1 (Figs. 2c and 4a). Association of Snail1-HA with these additional elements was confirmed by ChIP-qPCR (Fig. 4b) and EMSA (Fig. 4c, Supplementary Fig. S14). Thus, Snail1-HA can directly interact with specific DNA sequences at multiple positions of the MYB locus. To learn more about the mechanism whereby Snail1-HA represses MYB, we next investigated changes in chromatin structure and the abundance of histone marks typically found at active promoter and enhancer elements (H3K27ac), at poised and active enhancer elements (H3K4me1), and at promoter regions (H3K4me3). Using formaldehyde-assisted isolation of regulatory elements (FAIRE) [39], we found an open chromatin conformation around the MYB TSS, and the +2.7 and +85 kb regions in control LS174T and HT29 cells (Fig. 4d). Upon expression of Snail1-HA, chromatin structure at these regions adopted a closed conformation in both cell lines.
When we assessed histone marks at the MYB locus in the absence of Snail1-HA, we observed high levels of H3K27ac at several regions (Fig. 4e). Likewise, H3K4me3 was strongly represented around the TSS, whereas H3K4me1 accumulated at the MYB +2.7 and +85 kb regions, marking them as potential enhancer elements. Upon induction of Snail1-HA, H3K27ac levels diminished, especially at the +85 kb region. H3K4me1 and H3K4me3 seemingly were not affected by Snail1-HA. We conclude that Snail1-HA represses MYB by abolishing chromatin features characteristic for actively transcribed genes, but leaves the locus in a poised state.
Loss of MYB impairs viability and clonogenicity of CRC cells
Next, we investigated how knockdown and knockout of MYB affected the phenotype of two CRC cell lines (LS174T and LS411) with fairly high levels of MYB expression (Figs. 3 and 5; Supplementary Figs. S15, S16). MYB knockdown/knockout and control cells were analyzed with respect to viability, apoptosis, proliferation, and two- and three-dimensional colony formation. Since LS411 cells did not form colonies in soft agar, we assessed their three-dimensional growth using a limiting dilution assay with ultralow attachment plates. This additionally provides information about the frequency of sphere-forming units as measure of stem cell numbers [40]. MYB knockdown/knockout resulted in decreased MTT conversion (Fig. 5b, Supplementary Fig. S16b). This is likely due to impaired proliferation rather than increased cell death (Fig. 5c, d; Supplementary Fig. S16c, d). Furthermore, MYB knockdown/knockout diminished colony numbers in two-dimensional growth conditions (Fig. 5e; Supplementary Fig. S16e). Likewise, MYB loss-of-function impaired anchorage-independent growth (Fig. 5f; Supplementary Fig. S16f). Furthermore, stem cell frequency was significantly decreased. We did notice, though, that one of the three MYB knockout clones obtained, behaved like wild-type cells in all analyses. Possibly, 2F4 cells suffered additional genome changes that mask the consequences of MYB loss-of-function. Nonetheless, the functional analyses support an important role for MYB in the regulation of proliferation and clonogenicity of CRC cells.
Discussion
Here, we determined the chromosomal distribution of Snail1-HA in a CRC EMT model to identify novel SNAIL1-regulated genes and to obtain deeper insights into gene-regulatory mechanisms and phenotypic changes associated with SNAIL1-induced EMT. Among the genes associated with Snail1-HA ChIP-seq peaks were several well-known SNAIL1 targets. This validated the reliability of our data set and allowed the confident identification of novel target genes such as WiNTRLINC1 and MYB. Furthermore, almost all Snail1-HA ChIP-seq peaks harbored the cognate SNAIL1 DNA-binding motif. In several cases we confirmed specific interactions of Snail1-HA with this motif in vitro. Apparently, in our model Snail1-HA interacts with the genome predominantly through its intrinsic sequence-specific DNA-binding capacity. In contrast, a recent study suggested that SNAIL1 may function through an alternative DNA sequence motif in BRCA cells [11]. This discrepancy could reflect direct versus indirect ways of chromosomal association. Such a piggyback mode of target gene access was previously reported for EMT inducers, possibly distinguishing repressive from activating target gene interactions [41, 42].
Comparison with previous studies indicates that SNAIL1 proteins occupy different genomic locations in different cellular backgrounds [10,11,12, 43, 44]. SNAIL1 and SLUG chromosomal distribution also differs despite identical DNA-binding specificity [5, 10]. Evidently, the range of DNA sequences that are occupied by SNAIL1 proteins and other EMT inducers is highly context-dependent. Contextual patterns of DNA occupancy could be determined by cell-type-specific interaction partners, but also by epigenetic factors. Significantly, regulatory elements bound by SNAIL1 proteins reside in an open chromatin conformation and are associated with active histone marks before the appearance of SNAIL1 proteins (this study, refs. [9, 12]). Accordingly, which genes and DNA elements are available for occupancy by SNAIL1 could be decisively determined by cell-type-specific preexisting chromatin landscapes. Likewise, Snail1-HA-induced gene repression was accompanied by changes in chromatin structural features (this study, refs. [9, 12]). Interestingly, at the MYB locus, H3K4me1, and H3K4me3 were unaffected by Snail1-HA. Apparently, MYB regulatory elements maintain a poised state during EMT which might allow for rapid MYB reactivation. This could be of significance for metastatic colonization which is thought to require mesenchymal-to-epithelial transition and resuscitation of epithelial gene expression [3].
Snail1-HA and ASCL2 ChIP-seq peaks frequently colocalized and both factors share highly similar DNA-recognition motifs [5]. Accordingly, Snail1-HA may displace ASCL2 and similar basic helix-loop-helix factors from regulatory DNA elements by competing for shared binding sites [9, 43]. This might also apply to the WiNTRLINC1 locus, even though expulsion of ASCL2 was incomplete at this gene. However, WiNTRLINC1 experienced only transient downregulation ins LS174T cells. Hence, the limited resolution of ChIP-qPCR experiments may have prevented detection of temporary ASCL2 dissociation from the WiNTRLINC1 locus. Thus, mutually exclusive occupancy of transcription factor binding sites likely represents an important mechanism whereby SNAIL1 proteins inactivate regulatory regions. The case of the MYB +85 kb element with nonoverlapping-binding sites for Snail1-HA and ASCL2 hints that SNAIL1 proteins engage additional, more indirect mechanisms for target inactivation, possibly based on chromatin structural changes [45, 46].
MYB, while best known for its role in hematopoiesis and hematological disorders, is also important for intestinal development, and functions as oncogene in solid cancers [47,48,49,50,51,52]. In agreement with its tumor-promoting capacity, MYB loss-of-function impaired viability, clonogenicity, and anchorage-independent growth of CRC cells. Nonetheless, quite counterintuitively, higher MYB expression levels correlated with a better prognosis for colorectal adenocarcinoma patients. Likewise, repression of MYB by Snail1-HA may seem surprising as this would attenuate oncogenic transformation. However, EMT is known to entail reduced cell proliferation [53] which most likely is a necessary corollary of increased motility. Thus, repression of MYB and possibly other oncogenes during EMT is plausible and could occur more frequently. Support for this comes from the observed anticorrelated expression of SNAIL1 and MYB in CRC and BRCA transcriptomes, and the repression of MYB also by ZEB1 [54]. Furthermore, comparatively higher MYB expression levels appear to be a characteristic of more epithelial tumors, while relatively lower MYB expression is found in more mesenchymal tumors, which in fact are those with a worse prognosis [55].
Knowledge about the cellular origin of CRCs and the extensive characterization of ISCs concerning essential transcription factors, key regulatory circuits of ISC fate, and distinguishing gene expression signatures [19,20,21,22,23, 56, 57] allowed us to examine at a molecular level how SNAIL1 proteins affect stemness features. The observed repression of multiple genes of crucial importance for ISCs [37, 56, 58] argues that SNAIL1-induced EMT interferes with stemness aspects of CRC cells. This is consistent with several reports demonstrating that stemness and tumor-initiating capacities are not necessarily linked to mesenchymal cell fates [9, 17, 18, 59]. Yet, SNAIL1 proteins and EMT have repeatedly been reported to promote stemness features of cancer cells [3, 13,14,15,16, 60, 61]. These contradictions might be reconciled in several ways. There might be different types of stemness installed by different combinations of transcription factors [10, 62]. Alternatively, a single genetic program that confers the defining properties of stem cells may be variably controlled by epithelial as well as mesenchymal collectives of transcription factors. Lastly, experimentally induced complete EMT may indeed abrogate stemness, whereas carcinogenesis possibly selects for more plastic, intermediate EMT states that amalgamate stemness aspects of epithelial cell states and higher mobility and invasiveness of mesenchymal cells [3].
In summary, our high confidence collection of SNAIL1-bound chromosomal regions represents a valuable resource for future studies aiming at a molecular-mechanistic dissection of EMT processes. As a paradigm, we analyzed the impact of SNAIL1 on a genetic program that underlies the ISC state. Our results hint at a complex interplay between EMT and stemness which warrants further investigations of the corresponding gene-regulatory circuits in cells with varying degrees of epithelial and mesenchymal characteristics.
Materials and methods
Cell culture
Cell lines (listed in Supplementary Table S9) were cultivated in DMEM with 10% (v/v) FCS, 10 mM HEPES, 1% (v/v) MEM nonessential amino acids, and 1% (v/v) penicillin/streptomycin at 37 °C and 5% CO2. MCF10A cells were cultivated in Advanced DMEM/F12 with 5% (v/v) Horse serum, 1% (v/v) (penicillin/streptomycin), 20 ng/ml human EGF, 0.5 μg/ml hydrocortisone, 0.1 μg/ml cholera toxin, and 10 μg/ml insulin at 37 °C and 5% CO2.
Oligonucleotides and antibodies
All oligonucleotides and antibodies used are listed in Supplementary Tables S10 and S11.
RNA isolation, cDNA synthesis, and qRT-PCR
RNA was isolated and reverse transcribed for quantitative gene expression by qRT-PCR as described [8] using GAPDH transcripts for normalization.
Protein extraction and western blotting
For detection of MYB, SNAIL1, and SLUG, nuclear extracts were prepared [8]. All other proteins were analyzed using whole cell lysates [28]. Detection was performed as described [63].
Dox-inducible shRNA expression
ShRNAs were selected based on top hits for MYB [64]. For cloning, 97mer oligonucleotides were PCR amplified, digested with EcoRI and XhoI and inserted into the pTRIPZ-vector (OpenBiosystems). The resulting lentiviral vectors were used for infection of LS174T cells as described [8]. Transduced cells were selected using 6 µg/ml blasticidin.
Genome editing
An online tool (http://crispr.mit.edu) [65] was employed to design gRNAs which were cloned into the gRNA expression vector (a gift from George Church, #41824, addgene, Cambridge, MA, USA) [66]. For genome editing, 2 × 106 cells were transfected with 0.5 µg of a Cas9-GFP construct (a gift from Kiran Musunuru, #44719, addgene, Cambridge, MA, USA), 0.5 µg gRNA expression vector, and 0.5 µg of dsRed expression vector using the Cell Line Nucleofector kit L (#VCA-1005, Lonza, Cologne, Germany). GFP/RFP double positive cells were single cell sorted 72 h post nucleofection, emerging cell clones were expanded, genotyped, and monitored for protein expression prior to phenotypic testing.
DNA binding in vitro
EMSAs were performed as described [9].
Formaldehyde-assisted isolation of regulatory elements (FAIRE)
FAIRE and calculation of the relative FAIRE enrichment was performed as described [8]. To purify DNA, the peqGOLD Cycle-Pure kit (011917, VWR, Darmstadt, Germany) was used according to the manufacturer’s protocol. For qPCR analyses, 40 ng of purified DNA served as template.
ChIP-qPCR, ChIP-seq, and data processing
ChIP-qPCR was performed as before [8]. For immunoprecipitation with histone antibodies, aliquots of 100 µg chromatin were used. Chromatin for ChIP-seq was prepared as described [8], except that cells were crosslinked for 5 min, and chromatin was sheared to 100–550 bp fragments by sonication for 12 min in a Covaris S220 device (Covaris, Woburn, MA, USA) with the following settings: peak incident power 150 W, duty factor 10%, and cycles/burst 200. After shearing, lysates were cleared by centrifugation (16 000 × g, 10 min, and 4 °C). The chromatin concentration in the supernatant was measured using a NanoDrop 2000 device (Thermo Fisher Scientific, Dreieich, Germany). Immunoprecipitations with 200 µg chromatin and 1 µg anti-HA antibody, and the subsequent washing steps were carried out as described [8]. To collect sufficient material for sequencing, 10–15 ChIPs were done in parallel. Samples were pooled before purifying immunoprecipitated DNA. DNA content was measured using the Qubit 2.0 system (Invitrogen, Paisley, UK). Two independent pools (=replicates) were prepared and submitted for library generation and subsequent 50 bp single-end sequencing on an Illumina HiSeq2000 (Illumina, San Diego, CA, USA). Bad quality reads were removed using trimmomatic [67]. Reads from ChIP and input samples were aligned to the human reference genome (hg19) with BWA aligner [68]. GATK was used for post processing analyses including local realignment and base quality score recalibration [69]. Peaks were called using the MACS2 software [70]. For each replicate, peaks were called on the aligned reads of ChIP samples using the corresponding input sample for background normalization. Peaks with an adjusted p-value below 0.05 were set as significant. The ChIPseeker R package was employed to assess the relation to closest gene and gene region annotations [71]. Bam files were converted to bigwig files using Galaxy and bamCoverage [72]. BigWig files were visualized with the UCSC genome browser [73]. ChIP-seq data files were deposited in GEO under the accession ID GSE127183.
Motif enrichment and GSEA
De novo motif analysis was performed using the rGADEM R packages [74]. The two ChIP-seq replicates were analyzed separately, an unseeded search was run with p-value and e-value parameters set to 0.0002 and zero, respectively. Motifs discovered were then mapped to known position weight matrices from the human transcription factor database [5] using MotIV R package [75]. The best match per motif was selected according to its e-value. GSEA of ChIP-seq peak-associated genes was examined separately for the two replicates using a hypergeometric test with the whole set of human protein-coding genes as background. GSEA was performed based on the Gene Ontology database “Biological Processes” and the ISC gene signature [21]. The Benjamini–Hochberg method was used for multiple-testing correction.
To compare different ChIP-seq data sets, Snail1-HA ChIP-seq replicates were merged using the “union” method from GenomicRanges R package [76]. For subsequent overlap analysis, Snail1-HA, ASCL2, and TCF7L2 ChIP-seq data sets were converted to hg38, using the UCSC liftover tool (https://genome.ucsc.edu/cgi-bin/hgLiftOver). For each gene region, we quantified the overlap between peak-associated genes in our data set and the published data sets [29, 77]. For Snail1 and Slug ChIP-seq data from murine cells, we filtered out peak-associated genes missing human homologs [10]. Significance of the overlap was assessed using a hypergeometric test with the sum of peak-associated genes in the tested sample as background.
Colony formation assays
For 2D colony formation assays, 1 × 103 cells/well were seeded in six-well plates in 2 ml DMEM with supplements. Medium, and if applicable Dox, was refreshed every 48 h. After 12 days of cultivation, medium was removed, cells were washed once with PBS and then stained with 1% crystal violet in 20% methanol for 10 min. Thereafter, the crystal violet solution was removed, cells were washed three times with H2O and then dried. Colonies were counted using the ImageJ software. For 3D colony formation assays, 2 × 103 cells/well were mixed with a top agar solution consisting of 0.7% sea plaque agarose (Lot 0000559478, Lonza, Basel, Switzerland) in DMEM with supplements. The cell suspension was seeded onto a base agar consisting of 1% sea plaque agarose in DMEM with supplements in 96-well plates. After solidification, the agar was overlaid with DMEM with supplements. Media and Dox were renewed every second day. Cells were cultured for 14 days, pictures were taken with a Nikon Eclipse TS100 microscope equipped with a Nikon DS-Qi1MC camera and colony numbers were determined. Colony diameter was measured using ImageJ software.
Limiting dilution assay
Cell numbers ranging from 1000 to 0.01 cells/well were seeded and cultivated in 96-well ultralow attachment plates. For each cell number, eight replicates were prepared. Media was refreshed every second day. Fourteen days after seeding the number of spheroid-containing wells per octaplicate were determined and subsequently processed using the ELDA tool (https//bioinf.wehi.edu.au/software/elda/) [40].
MTT assay
For MTT assays, 1 × 103 cells/well were seeded in 100 µl DMEM with supplements in 96-well plates. For each time point and condition, three wells were prepared as technical replicates. Where applicable, cells received 1 µg/ml Dox. Dox was refreshed after 48 h. 24 h (LS411 only) and 96 h after seeding, media were replaced by 20 µl of 5 mg/ml MTT in DMEM. Cells were incubated at 37 °C for 1 h, followed by removal of the supernatant. Cells were dissolved and the insoluble dye was extracted with 150 µl DMSO/well. The extinction of the solution was measured at 540 nm and 670 nm wavelength. For normalization, 670 nm values were subtracted from 540 nm values.
Flow cytometry
Cells were washed once with PBS and then trypsinized. Trypsinization was stopped by adding DMEM with supplements. Cells were centrifuged for 5 min at 150 × g, the supernatant was removed, cells were washed once with PBS, vortexed, and spun down again. The cells were then fixed in 70% ice-cold ethanol and stored at 4 °C. Prior to flow cytometry, the cell suspension was vortexed and then spun down at 150 × g. The supernatant was removed and the cell pellet was washed with 1× PBS. The washing step was repeated once before the cells were incubated with 20 µg/ml propidium iodide and 10 µg/ml RNAse in PBS for 30 min at 37 °C. Thereafter, the cell suspension was vortexed, spun down, washed with PBS, and resuspended in 200 µl to 400 µl PBS according to pellet size. Flow cytometric measurements were done with a CytoFlex S (Beckman Coulter, Indianapolis, USA).
Analysis of transcriptome data
Genes differentially expressed in the presence of Snail1-HA and bound by Snail1-HA, ASCL2, and TCF7L2, were identified upon processing the microarray data set GSE115716 as described [8]. Differentially expressed genes showing log2 fold changes of <−0.5 for downregulation, and >0.5 for upregulation (p-values < 0.05), respectively, were selected, and their association with ChIP-seq peaks for Snail1-HA, ASCL2, and TCF7L2 was analyzed at each time point post Snail1-HA induction. Note, that occupancy by Snail1-HA was determined only at t = 6 h. Kaplan–Meier plots were created using the XenaBrowser (http://xenabrowser.net). Studies selected for analyses were TCGA colon and rectal adenocarcinoma (COADREAD), BRCA, and pan-cancer (PANCAN). As gene-of-interest, MYB was picked. As assay type “IlluminaHiSeq gene expression RNAseq” was chosen. Samples were filtered for primary tumors. Pairwise-correlation analyses and calculation of relative gene expression levels based on CRC (GSE14333, GSE39582) and BRCA microarray data (TCGA BRCA) were performed as described [27, 28].
Statistics
Statistical analysis was performed using an unpaired, two-tailed Student’s t test. Similarity of variances was assessed by the F-test function implemented in GraphPad Prism v6.0. Unless otherwise stated, the comparison was between Dox-treated versus untreated cells and between parental versus genome-edited cells. Significant changes are shown by the respective p-values represented with *p < 0.05; **p < 0.01; ***p < 0.001. Non-significant changes: ns. Data are presented as mean + SEM. The sample size (=number of independent biological replicates) of each distinct experiment is indicated in the corresponding figure legend.
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Acknowledgements
The authors are grateful to K. Geiger and D. Herchenbach for excellent technical assistance, and to the team of the Genomics and Proteomics Core Facility, German Cancer Research Center/DKFZ, Heidelberg, Germany, for their sequencing service. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG HE2004/11-1 to AH; CRC-850 subprojects B5 to AH and Z1 to MB; GRK2344 subprojects B02 to MB and B03 to AH), and by the German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept CoNfirm (FKZ 01ZX1708F to MB). JW and PA-G were funded by the Deutsche Forschungsgemeinschaft Research Training Group GRK2344 “MeInBio—BioInMe”.
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Beyes, S., Andrieux, G., Schrempp, M. et al. Genome-wide mapping of DNA-binding sites identifies stemness-related genes as directly repressed targets of SNAIL1 in colorectal cancer cells. Oncogene 38, 6647–6661 (2019). https://doi.org/10.1038/s41388-019-0905-4
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DOI: https://doi.org/10.1038/s41388-019-0905-4
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