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CHRONIC MYELOPROLIFERATIVE NEOPLASMS

Deregulation of the p19/CDK4/CDK6 axis in Jak2V617F megakaryocytes accelerates the development of myelofibrosis

Leukemia volume 38, pages 898–902 (2024)Cite this article

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Fig. 1: P19Ink4d depletion favors the rapid development of splenomegaly and MF in Jak2V617F context trough deregulation of megakaryopoiesis.
Fig. 2: Palbociclib treatment prevents myeloproliferation, splenomegaly and MF development in Jak2V617F context.

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Acknowledgements

We thank P. Rameau, C. Catelain, Y. Lecluse, T. Manoliu from the Imaging and cytometry platform PFIC and S. Souquere from Electronic microscopy platform, UMS3655-AMMICA, Gustave Roussy Villejuif, France; to O. Bawa, H. Rocheteau and N. Signolle for immunohistochemistry and immunofluorescence, and C. Metereau, L. Touchard for help with experiments on animals from preclinical research platform, PETRA, Gustave Roussy Villejuif, France; and to E. Pascal from INSERM U1176 for vWF dosage. This work was supported by grants from Ligue Nationale Contre le Cancer (Equipe labellisée 2016, 2019 and 2022 to HR) and MPN research foundation (IP). LG was supported by a fellowship from Association pour la recherche contre le cancer (ARC, France).

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Author notes

  1. These authors contributed equally: Delphine Muller, Laure Gilles.

Authors and Affiliations

  1. INSERM, UMR1287, Gustave Roussy, Equipe labellisée Ligue Nationale Contre le Cancer, Villejuif, France

    Hélène Duparc, Delphine Muller, Laure Gilles, Agathe L. Chédeville, Rose Guignard, Najet Debili, Monika Wittner, Florence Pasquier, Iléana Antony-Debré, Caroline Marty, William Vainchenker, Isabelle Plo & Hana Raslova

  2. Université Paris-Saclay, Gif-sur-Yvette, Villejuif, France

    Hélène Duparc, Delphine Muller, Laure Gilles, Agathe L. Chédeville, Rose Guignard, Najet Debili, Monika Wittner, Florence Pasquier, Iléana Antony-Debré, Caroline Marty, William Vainchenker, Isabelle Plo & Hana Raslova

  3. Gustave Roussy, Villejuif, France

    Hélène Duparc, Delphine Muller, Laure Gilles, Agathe L. Chédeville, Rose Guignard, Najet Debili, Monika Wittner, Iléana Antony-Debré, Caroline Marty, William Vainchenker, Isabelle Plo & Hana Raslova

  4. Université Paris Cité, Paris, France

    Hélène Duparc, Agathe L. Chédeville & Rose Guignard

  5. INSERM UMRS 938, PARIS, Sorbonne Université, Centre de Recherche Saint- Antoine, AP-HP, Hôpital Saint-Antoine, Paris, France

    Mira El Khoury

  6. INSERM U1176, Hemostasis, Inflammation & Thrombosis (HITh), Université Paris-Saclay, Le Kremlin-Bicêtre, France

    Alexandre Kauskot

  7. Département d’Hématologie, Gustave Roussy, Université Paris-Saclay, Villejuif, France

    Florence Pasquier

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Contributions

HD, DM, LG, ALC, MEK, AK and IAD performed and analyzed experiments. FP provided patient samples. HD, DM, IAD, LG, ND, AK, FP, CM, RG, MW, VW, IP, HR discussed results. HR supervised the work. HD, DM, LG, VW, IP and HR wrote the article. DM and LG equally contributed to this work.

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Correspondence to Hana Raslova.

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Duparc, H., Muller, D., Gilles, L. et al. Deregulation of the p19/CDK4/CDK6 axis in Jak2V617F megakaryocytes accelerates the development of myelofibrosis. Leukemia 38, 898–902 (2024). https://doi.org/10.1038/s41375-024-02170-5

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