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ACUTE LYMPHOBLASTIC LEUKEMIA

Role of transcriptome sequencing in clinical diagnosis of B-cell acute lymphoblastic leukemia

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Fig. 1: Summary of the results of the first 21 patients enrolled in the Ma-Spore ALL 2020 study by RNA-Seq diagnostic workflow.

References

  1. 1.

    van der Velden VHJ, Brüggemann M, Cazzaniga G, Scheijen B, Tops B, Trka J, et al. Potential and pitfalls of whole transcriptome-based immunogenetic marker identification in acute lymphoblastic leukemia; a EuroMRD and EuroClonality-NGS Working Group study. Leukemia. 2021;35:924–8.

  2. 2.

    Li Z, Jiang N, Lim EH, Chin WHN, Lu Y, Chiew KH, et al. Identifying IGH disease clones for MRD monitoring in childhood B-cell acute lymphoblastic leukemia using RNA-Seq. Leukemia. 2020;34:2418–29.

    Article  Google Scholar 

  3. 3.

    Bolotin DA, Poslavsky S, Mitrophanov I, Shugay M, Mamedov IZ, Putintseva EV, et al. MiXCR: software for comprehensive adaptive immunity profiling. Nat Methods. 2015;12:380–1.

    CAS  Article  Google Scholar 

  4. 4.

    Chang YF, Imam JS, Wilkinson MF. The nonsense-mediated decay RNA surveillance pathway. Annu Rev Biochem. 2007;76:51–74.

    CAS  Article  Google Scholar 

  5. 5.

    Inaba H, Azzato EM, Mullighan CG. Integration of next-generation sequencing to treat acute lymphoblastic leukemia with targetable lesions: The St. Jude Children’s Research Hospital Approach. Front Pediatrics. 2017;5:258.

    Article  Google Scholar 

  6. 6.

    Tran TH, Loh ML. Ph-like acute lymphoblastic leukemia. Hematol Am Soc Hematol Educ Program. 2016;2016:561–6.

    Article  Google Scholar 

  7. 7.

    Schieck M, Lentes J, Thomay K, Hofmann W, Behrens YL, Hagedorn M, et al. Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia. Ann Hematol. 2020;99:809–18.

  8. 8.

    Van der Auwera GA, Carneiro MO, Hartl C, Poplin R, Del Angel G, Levy-Moonshine A, et al. From FastQ data to high confidence variant calls: the Genome Analysis Toolkit best practices pipeline. Curr Protoc Bioinforma. 2013;43:11.10.1–33.

    Article  Google Scholar 

  9. 9.

    Nicorici D, Satalan M, Edgren H, Kangaspeska S, Murumagi A, Kallioniemi O, et al. FusionCatcher—a tool for finding somatic fusion genes in paired-end RNA-sequencing data. bioRxiv. 2014. https://doi.org/10.1101/011650.

  10. 10.

    Lee VS, Kawamoto K, Hess R, Park C, Young J, Hunter C, et al. Implementation of a value-driven outcomes program to identify high variability in clinical costs and outcomes and association with reduced cost and improved quality. JAMA. 2016;316:1061–72.

    Article  Google Scholar 

Download references

Acknowledgements

The computational work for this article was partially performed on resources of the National Supercomputing Centre, Singapore (https://www.nscc.sg). This study is supported by the Singapore National Medical Research Council Clinician Scientist Awards (NMRC/CSA/0053/2013 and MOH-000277), Children’s Cancer Foundation (Singapore), Singapore Tote Board, Goh Foundation (Singapore) and Viva Foundation for Children with Cancer.

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Correspondence to Allen Eng-Juh Yeoh.

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Li, Z., Jiang, N., Lim, E.H. et al. Role of transcriptome sequencing in clinical diagnosis of B-cell acute lymphoblastic leukemia. Leukemia (2021). https://doi.org/10.1038/s41375-021-01185-6

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