Acute myeloid leukemia

Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2

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Acknowledgements

We thank Dr. Colin E. Correnti and the Molecular Design and Therapeutics core facility team (Fred Hutchinson Cancer Research Center) for the generation of AMG 330. We also thank Helen Crawford for help preparing and formatting this manuscript, and Jerry Chen for general mouse colony maintenance. Research reported in this publication was supported by the Leukemia & Lymphoma Society (Translational Research Program, grant 6489-16). RBW is a Leukemia & Lymphoma Society Scholar in Clinical Research. H-PK is a Markey Molecular Medicine Investigator and received support as the inaugural recipient of the José Carreras/E. Donnall Thomas Endowed Chair for Cancer Research and the Fred Hutchinson Cancer Research Center Endowed Chair for Cell and Gene Therapy.

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Correspondence to Olivier Humbert.

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RBW has received laboratory research grants and/or clinical trial support from Actinium Pharmaceuticals, Inc, Amgen Inc., Amphivena Therapeutics, Inc., Covagen AG, and Seattle Genetics, Inc.; has ownership interests with Amphivena Therapeutics, Inc.; and is (or has been) a consultant to Amphivena Therapeutics, Inc., Boehringer Ingelheim Pharma GmbH & Co. KG, Covagen AG, Pfizer, Inc., and Seattle Genetics, Inc. H-PK is a consultant for Rocket Pharma and Homology Medicines. The other authors declare that they have no conflict of interest.

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Humbert, O., Laszlo, G.S., Sichel, S. et al. Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2. Leukemia 33, 762–808 (2019). https://doi.org/10.1038/s41375-018-0277-8

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