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Roux-en-Y Gastric-Bypass and sleeve gastrectomy induces specific shifts of the gut microbiota without altering the metabolism of bile acids in the intestinal lumen


Some shifts in the gut microbiota composition and its metabolic fingerprints have been associated to Sleeve gastrectomy (SG) and Roux-en-Y Gastric Bypass (RYGB). So far, plasma bile acids have been associated with post-operative glucose improvement and weight loss, but nothing is known about their metabolism in the gut lumen. As bile acids are physiologically transformed by the microbiota into various species, the aim of this work was to study how SG and RYGB-associated dysbiosis impact the bioconversion of bile acids in the intestinal lumen. Comparing SHAM (n = 9) with our validated rat models of SG (n = 5) and RYGB (n = 6), we quantified luminal bile acids along the gut and found that the metabolic transformation of bile acids (deconjugation, dehydroxylation, and epimerization) is not different from the duodenum to the colon. However, in the cecum where the biotransformation mainly takes place, we observed deep alterations of the microbiota composition, which were specific of each type of surgery. In conclusion, despite specific dysbiosis after surgery, the bile acids metabolism in the gut lumen is highly preserved, suggesting that a resilience of the gut microbiota occurs after these procedures.

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  1. Kohli R, et al. Weight loss induced by Roux-en-Y gastric bypass but not laparoscopic adjustable gastric banding increases circulating bile acids. J Clin Endocrinol Metab. 2013;98:E708–12.

    Article  CAS  Google Scholar 

  2. Ahmad NN, Pfalzer A, Kaplan LM. Roux-en-Y gastric bypass normalizes the blunted postprandial bile acid excursion associated with obesity. Int J Obes. 2013;37:1553–9.

    Article  CAS  Google Scholar 

  3. Pournaras DJ, et al. The role of bile after Roux-en-Y gastric bypass in promoting weight loss and improving glycaemic control. Endocrinology. 2012;153:3613–9.

    Article  CAS  Google Scholar 

  4. Penney NC, Kinross J, Newton RC, Purkayastha S. The role of bile acids in reducing the metabolic complications of obesity after bariatric surgery: a systematic review. Int J Obes. 2015;39:1565–74.

    Article  CAS  Google Scholar 

  5. Gralka E, et al. Metabolomic fingerprint of severe obesity is dynamically affected by bariatric surgery in a procedure-dependent manner. Am J Clin Nutr. 2015;102:1313–22.

    Article  CAS  Google Scholar 

  6. Murphy R. Differential adaptation of human gut microbiota to weight loss and diabetes remission achieved by gastric bypass versus sleeve gastrectomy, EASD meeting, Stockholm, Sweden. 2015.

  7. Chávez-Talavera O, et al. Roux-en-Y gastric bypass increases systemic but not portal bile acid concentrations by decreasing hepatic bile acid uptake in minipigs. Int J Obes. 2017;41:664–8.

    Article  Google Scholar 

  8. Hofmann AF. Bile acids: the good, the bad, and the ugly. News Physiol Sci. 1999;14:24–9.

    CAS  PubMed  Google Scholar 

  9. Ridlon JM, Kang D-J, Hylemon PB. Bile salt biotransformations by human intestinal bacteria. J Lipid Res. 2006;47:241–59.

    Article  CAS  Google Scholar 

  10. Arapis K, et al. Remodeling of the residual gastric mucosa after roux-en-y gastric bypass or vertical sleeve gastrectomy in diet-induced obese rats. PLoS ONE. 2015;10:e0121414.

    Article  Google Scholar 

  11. Liguori G, et al. Fungal dysbiosis in mucosa-associated microbiota of Crohn’s disease patients. J Crohns Colitis. 2015;10:296–305.

    Article  Google Scholar 

  12. Schmieder R, Edwards R. Quality control and preprocessing of metagenomic datasets. Bioinformatics. 2011;27:863–4.

    Article  CAS  Google Scholar 

  13. Magoč T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011;27:2957–63.

    Article  Google Scholar 

  14. Sacquet EC, et al. Bacterial formation of omega-muricholic acid in rats. Appl Environ Microbiol. 1979;37:1127–31.

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Risstad H, et al. Bile acid profiles over 5 years after gastric bypass and duodenal switch: results from a randomized clinical trial. Surg Obes Relat Dis. 2017;13:1544–53.

    Article  Google Scholar 

  16. Fouladi F, Mitchell JE, Wonderlich JA, Steffen KJ. The contributing role of bile acids to metabolic improvements after obesity and metabolic surgery. Obes Surg. 2016;26:2492–502.

    Article  Google Scholar 

  17. Caporaso JG, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.

    Article  CAS  Google Scholar 

  18. Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010;26:2460–1.

    Article  CAS  Google Scholar 

  19. McDonald D, et al. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J. 2012;6:610–8.

    Article  CAS  Google Scholar 

  20. Hirano S, Masuda N, Oda H, Mukai H. Transformation of bile acids by Clostridium perfringens. Appl Environ Microbiol. 1981;42:394–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Duboc H, et al. Connecting dysbiosis, bile-acid dysmetabolism and gut inflammation in inflammatory bowel diseases. Gut. 2013;62:531–9.

    Article  CAS  Google Scholar 

  22. Ussar S, et al. Interactions between gut microbiota, host genetics and diet modulate the predisposition to obesity and metabolic syndrome. Cell Metab. 2015;22:516–30.

    Article  CAS  Google Scholar 

  23. Segata N, et al. Metagenomic biomarker discovery and explanation. Genome Biol. 2011;12:R60.

    Article  Google Scholar 

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This work was supported by the FARE grant from the “Société Nationale Francaise d’Hepato-Gastro-Entérologie”.

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Correspondence to Henri Duboc.

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The authors declare that they have no conflict of interest.

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Harry Sokol and André Bado are co-senior authors.

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Duboc, H., Nguyen, C.C., Cavin, JB. et al. Roux-en-Y Gastric-Bypass and sleeve gastrectomy induces specific shifts of the gut microbiota without altering the metabolism of bile acids in the intestinal lumen. Int J Obes 43, 428–431 (2019).

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