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Roux-en-Y Gastric-Bypass and sleeve gastrectomy induces specific shifts of the gut microbiota without altering the metabolism of bile acids in the intestinal lumen

International Journal of Obesity volume 43, pages 428–431 (2019)Cite this article

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Abstract

Some shifts in the gut microbiota composition and its metabolic fingerprints have been associated to Sleeve gastrectomy (SG) and Roux-en-Y Gastric Bypass (RYGB). So far, plasma bile acids have been associated with post-operative glucose improvement and weight loss, but nothing is known about their metabolism in the gut lumen. As bile acids are physiologically transformed by the microbiota into various species, the aim of this work was to study how SG and RYGB-associated dysbiosis impact the bioconversion of bile acids in the intestinal lumen. Comparing SHAM (n = 9) with our validated rat models of SG (n = 5) and RYGB (n = 6), we quantified luminal bile acids along the gut and found that the metabolic transformation of bile acids (deconjugation, dehydroxylation, and epimerization) is not different from the duodenum to the colon. However, in the cecum where the biotransformation mainly takes place, we observed deep alterations of the microbiota composition, which were specific of each type of surgery. In conclusion, despite specific dysbiosis after surgery, the bile acids metabolism in the gut lumen is highly preserved, suggesting that a resilience of the gut microbiota occurs after these procedures.

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Acknowledgements

This work was supported by the FARE grant from the “Société Nationale Francaise d’Hepato-Gastro-Entérologie”.

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Authors and Affiliations

  1. INSERM UMR 1149, University Paris Diderot, Sorbonne Paris Cité and DHU Unity, APHP, Paris, F-75890, France

    Henri Duboc, Caroline Chong Nguyen, Jean-Baptiste Cavin, Lara Ribeiro-Parenti, Anne-Charlotte Jarry, Maude Le Gall & André Bado

  2. Department of Hepato Gastro Enterology, Louis Mourier Hospital, APHP, University Paris Diderot, Sorbonne Paris Cité and DHU Unity, Paris, France

    Henri Duboc & Benoit Coffin

  3. Sorbonne Universités, UPMC Univ. Paris 06, École normale supérieure, PSL Research University, CNRS, INSERM, APHP, Laboratoire des Biomolécules (LBM), 27 rue de Chaligny, Paris, 75005, France

    Dominique Rainteau, Lydie Humbert & Harry Sokol

  4. INRA, UMR1319 Micalis & AgroParisTech, Jouy en Josas, France

    Harry Sokol

  5. Department of Gastroenterology, Saint Antoine Hospital, APHP, UPMC University Paris 06, Paris, France

    Harry Sokol

Authors
  1. Henri Duboc
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  2. Caroline Chong Nguyen
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  4. Lara Ribeiro-Parenti
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  8. Benoit Coffin
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Correspondence to Henri Duboc.

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The authors declare that they have no conflict of interest.

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Harry Sokol and André Bado are co-senior authors.

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Duboc, H., Nguyen, C.C., Cavin, JB. et al. Roux-en-Y Gastric-Bypass and sleeve gastrectomy induces specific shifts of the gut microbiota without altering the metabolism of bile acids in the intestinal lumen. Int J Obes 43, 428–431 (2019). https://doi.org/10.1038/s41366-018-0015-3

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