Deciphering the star codings: astrocyte manipulation alters mouse behavior


Astrocytes occupy a vast area within the central nervous system (CNS). Despite their abundance, the functional role of astrocytes in vivo has only begun to be uncovered. Astrocytes were typically thought to be involved in pathophysiological states. However, recent studies have shown that astrocytes are actively involved in cell signaling in normal physiological states; manipulating various aspects of astrocytic cell signaling in vivo has revealed that astrocytes are key players in controlling healthy behavior in the absence of pathophysiology. Unfortunately, the study of astrocyte function is often limited by the number of approaches available due to our lack of understanding of cell physiology. This review summarizes recent studies in which altered astrocyte signaling capacity resulted in dramatic changes in behavior. We not only discuss the methodologies available to manipulate astrocytes but also provide insights into the behavioral roles of astrocytes in the CNS.


Astrocytes, or “star-shaped” cells, which are as abundant in our CNS as neurons, densely and homogeneously populate the brain, spinal cord, and retina1,2. Although astrocytes are abundantly distributed across the entire brain, they have traditionally been regarded as “support cells” that play metabolic and homeostatic roles for nearby neurons3. The first evidence that neurons and astrocytes exchange functional signaling was presented ~30 years ago4,5,6. Later studies found that the star-shaped cells are particularly active in processing pain and inflammation7,8,9. Since then, studies have mainly focused on the role of astrocytes within the context of the brain’s pathophysiological states10,11. However, their role in physiological brain function in vivo has not been investigated for a while.

Over the last decade, there has been accumulating evidence that astrocytes actively participate in the brain’s physiological activities. Recent studies have also recognized the importance of the functional and regulatory roles that astrocytes play under normal physiological conditions. The final outputs that produce one’s behavior are generally accepted to be neuronal; however, astrocytes often critically modulate this final output. This relationship requires dynamic cell signaling between neurons and astrocytes. Morphologically, astrocytes have numerous processes that are in close proximity to neurons, other astrocytes, and blood vessels12. Astrocytic processes make contact with neuronal synapses and form tripartite synapses. A single astrocyte can make as many as 140,000 contacts with neuronal synapses13. Astrocytes actively exchange signals with nearby cells to complement and modulate neuronal communications in their vicinity. They often alter the electrophysiological properties of the surrounding area14. Therefore, it would not be unexpected for some of the astrocyte’s modulatory effects to influence behavioral outcomes in vivo. The hypothesis that astrocytes are directly involved in brain activity is further supported by recent studies showing how these cells are transcriptionally, translationally, morphologically, and functionally diverse across brain regions15. This evidence is important for understanding the more active roles of astrocytes under various physiological states. Efforts to uncover the effects of astrocytes have yielded valuable insights and tools for researchers. Consequently, astrocytes are no longer regarded as passive auxiliary support cells that function uniformly throughout the CNS.

Despite such advances and endeavors, however, a large portion of astrocyte physiology remains vague, and it is unclear how astrocytes process information to affect behavior16. Therefore, studies are needed to characterize astrocyte functions as well as their roles in the modulation of various behaviors. In this review, we highlight recent studies in which artificial disruption or enhancement of astrocyte cell signaling capacity in vivo resulted in immediate behavioral changes. These studies used experimental designs including but not limited to pharmacological, genetic, optogenetic, and chemogenetic approaches to specifically target astrocyte signaling processes. Additionally, we summarize the evidence for and provide current perspectives on the roles that astrocytes play in cell signaling and their critical impacts on healthy behaviors in rodents.

Astrocytic cell signaling

Manipulating astrocytes requires a profound understanding of their signaling processes because each step may be important in understanding astrocyte function. Astrocytes are not electrically excitable cells; nevertheless, they express wide range of functional neurotransmitter receptors17. One of the most prominent responses that astrocytes have to an external input is elevating intracellular Ca2+ levels. Following the first report of astrocytic Ca2+ elevation18, there have been multiple attempts to understand the dynamics, origins, and implications of this process. These attempts have included the use of fluorescent Ca2+ indicator dyes, such as fura-2, rhod-2, or fluo-4, and genetically encoded calcium indicators (GECIs), such as the calmodulin-containing GCaMP series. Fluorescent Ca2+ indicators have been used to uncover several important physiological properties of astrocytes19,20 and are still the primarily used indicators in in vitro studies. However, some studies have reported difficulties in maintaining uniform levels of the dyes across the entire cell over an extended period of time21. These dyes have also been reported to be toxic to cells22. Given the clear limitations to this approach, most in vivo studies use GECIs to measure Ca2+ elevations in astrocytes. GECIs are stably expressed over time and do not alter normal signaling levels or have detrimental effects on cells; therefore, they are particularly suitable for long-term in vivo recordings23. As the use of GCaMPs has become the standard calcium-measuring method for both neurons and astrocytes, the series has been continually adjusted to improve the resolution, localization, and stability24,25. While traditional GECIs allowed researchers to only observe a single Ca2+ dynamics, recent technological advancements have enabled multilayered approaches to imaging and measuring Ca2+ dynamics. Modified GECIs24 keep similar wavelengths from interfering with one another and are less sensitive to artificial stimuli such as light. Researchers are also able to mark neurons and astrocytes simultaneously using multicolored GECIs25 to deduce temporal associations between regional cell types.

Astrocytic Ca2+ arises from multiple sources. A major source of Ca2+ elevation is the endoplasmic reticulum, in which Ca2+ release is triggered via activated inositol triphosphate (IP3) receptors26. Interestingly, both Gq-coupled and Gi-coupled G-protein coupled receptors (GPCRs) have been shown to elevate Ca2+ levels in astrocytes27,28,29. While several studies that targeted the GPCR-IP3 pathway to control astrocytic Ca2+ activity have failed to identify any noticeable physiological effects30,31, other studies performed with Ip3r2−/− mice have found that this receptor is mainly responsible for somatic Ca2+ signals but not for Ca2+ signaling in cell processes32. This implies that there are other factors contributing to Ca2+ elevation in astrocytes, particularly in cell processes. Furthermore, the presence of spontaneous and seemingly random patches of Ca2+ in astrocytes is mediated by ion influx through transient receptor potential (TRP) A1 and V1 channels33,34. The TRPA1 channel is also responsible for maintaining resting Ca2+ levels33. More recently, it was shown that mitochondria mediate localized Ca2+ transients in astrocyte microdomains35. Taken together, these findings demonstrate that astrocyte Ca2+ signaling is highly dynamic. Assessing the implications of astrocyte function requires analysis with more spatiotemporal specificity, as well as comparisons of spontaneous and evoked, fast and slow, and global and focal signaling36. Nevertheless, studies that address the sources of astrocytic Ca2+ provide important information on potential targets for manipulating the astrocytic Ca2+ response.

After astrocytes receive signals and internally process them (primarily through Ca2+ elevation), they often emit signals to nearby cells. There is strong evidence that astrocytes are able to release signaling substances16 and that strong Ca2+ elevation precedes gliotransmitter release36. Gliotransmitters are neuroactive molecules released from astrocytes. These include glutamate, d-serine, ATP, and more. Upon their release into extracellular medium, gliotransmitters can exert an influence on local neurotransmission37. Findings suggest that astrocytes utilize SNARE-mediated vesicular exocytosis mechanisms for gliotransmission. Electron microscopy studies have detected gliotransmitters in labeled astrocytic vesicles38. In addition, soluble NSF attachment protein receptor (SNARE) isoforms have been identified in astrocytes, which also supports the vesicular-release model39. While the Ca2+ dynamics in astrocytes seem mainly asynchronous and oscillatory, one study demonstrated that gliotransmitter release is much more strongly correlated with a single large calcium elevation than with multiple small oscillatory waves40. Astrocytes have been shown to release d-serine to modulate long-term potentiation (LTP) in the CA1 region of the hippocampus41. They also release glutamate to induce timing-dependent long-term depression in the somatosensory cortex42 and release adenosine triphosphate (ATP) in response to mechanical stretch in the hippocampus43. It is not yet known whether a single astrocyte can release multiple neurotransmitters simultaneously or whether these cells can distinguish different input signals by differentiating their output transmitters. It also remains to be determined whether the release mechanism for each gliotransmitter is unique44. When astrocytes become reactive after injury or inflammation, their morphology and Ca2+ signaling patterns change drastically. Following a pathophysiological state in the brain, the amplitude and frequency of Ca2+ signals generally tend to increase43. In some models, astrocytic signals occur earlier than neuronal signals38. Although the detailed mechanism through which reactive astrocytes display heightened signaling is not completely understood, there is evidence that the aforementioned IP3 and TRPA1 pathways contribute in some way43. Such signaling complexities provide multiple areas of possible investigation to elucidate the consequences of astrocyte functions.

The approaches that are discussed in this review either enhance or impair one of the signaling pathways described above (Fig. 1). In genetic knockout models, such as Ip3r2−/− mice and Trpa1−/− mice, some of the known contributors to Ca2+ elevation in astrocytes are eliminated. Other studies genetically have introduced artificial pumps in astrocyte membranes to eliminate all intracellular calcium ions45. SNARE activity can also be modulated to regulate gliotransmission. Manipulation of designer receptors exclusively activated by designer drugs (DREADDs) has also been employed. These nonendogenous receptors are coupled with either Gq(hM3Dq) or Gi(hM4Di) to modulate astrocyte activity. However, there are reports that the Ca2+ levels in astrocytes increase in response to both Gq and Gi28,29, albeit to different degrees, and the specific differences between the two have not yet been characterized in vivo. Light-sensitive channelrhodopsin family proteins have also been used to study astrocytes. Although astrocytes are not electrically excitable, cation channels greatly increase Ca2+ levels in astrocytes46. Recently, the G-protein-coupled photopigment melanopsin was used for precise optogenetic astrocyte activation47. Admittedly, some of the methods employed by recent studies, especially optogenetic manipulation, have been brought into question48,49. As our understanding of the intercellular signals in astrocytes improves, there will be more layers to explore beyond those able to be studied with the currently available investigative approaches.

Fig. 1: Astrocyte signaling pathways and intervention points.

This schematic outlines some of the major signaling pathways in astrocytes. Changes in astrocytic intracellular Ca2+ levels depend on a number of activities by receptors, channels, pores, and other cellular components. Therefore, each component or process illustrated in this figure is also a possible intervention point to control intracellular Ca2+ levels. The components are roughly classified into several categories. Manipulating the activity of membrane proteins can be done using antagonists and blockers. GPCRs include mAChR, GABAB, and NMDAR, and the GPCR-IP3 pathway can also be regulated by controlling IP3R2 activity. Other membrane proteins, such as TRP channels, ion channels, and voltage-gated calcium channels (not shown), are also responsible for the Ca2+ response in astrocytes. Elevated astrocyte Ca2+ often leads to altered gene expression and SNARE-based gliotransmission. Although the gliotransmitter released varies between brain regions, dominant-negative SNARE proteins can be utilized to restrict gliotransmission.

Astrocyte manipulation in vivo

Astrocytes in circadian rhythm and sleep

One of the first behaviors that is suspected to be under astrocyte control is circadian rhythm, in part because the earliest understanding of astrocytes suggested that they regulate homeostatic control. The mammalian circadian system is derived from oscillatory regulation of transcription factors. It is imperative for organisms to maintain this timekeeping system to maintain concordance with the daily cycle. The circadian rhythm is thought to be supervised by neurons in the suprachiasmatic nucleus (SCN). These neurons express the transcription factors brain and muscle Arnt-like protein-1 (BMAL1) and circadian locomotor output cycles kaput (CLOCK) in a feedback loop fashion50. These neurons have been the focus of many circadian rhythm studies, while astrocytes have largely been overlooked. However, there is clear evidence that astrocytes rhythmically express circadian oscillators such as Bmal1 as well51. Astrocytes are also able to receive circadian inputs from nearby SCN neurons and release gliotransmitters in response51. It was uncertain whether astrocyte rhythmic activity led to circadian behavior until the recent work by Brancaccio et al.52. They used cryptochrome circadian regulator 1/2 (Cry1/2)-null mice, which are circadian-incompetent. Since CRY1 and CRY2 are necessary for a transcription–translation negative feedback loop (TTFL), Cry1/2-null mice have dysfunctional TTFL cycles and display no circadian behavior52. Cry1 was then selectively restored in SCN astrocytes or neurons by injecting Cre-dependent AAVs encoding Cry1::EGFP with GFAP-Cre or Syn-Cre AAV constructs. As expected, complementing Cry1 in SCN neurons quickly restored the TTFL, and the mice began exhibiting ~26-h behavior cycles52. Interestingly, complementing Cry1 only in the SCN astrocytes of Cry1/2-null mice was sufficient to induce the TTFL and restore circadian behavior52. This effect was largely blocked by inhibiting Cx43, an astrocyte-specific connexin hemichannel that is responsible for gliotransmitter release. In addition, treatment with the NMDA receptor antagonist DQP-1105 also blocked the rescue effect. These results suggest that astrocytic glutamate signaling is responsible for the restoration of circadian behavior52. In accordance with this, Tso et al.53 also focused on the function of SCN astrocytes in circadian signaling by specifically knocking out the circadian oscillator gene Bmal1 in SCN astrocytes using clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR-associated protein 9 (Cas9). Although astrocytes were shown to be functional circadian oscillators under normal conditions, the loss of Bmal1 disrupted the TTFL in astrocytes and significantly lengthened the circadian period53. Similarly, the presence of the casein kinase 1 epsilon (CK1ε) tau mutation specifically in astrocytes (Aldh1L1-CK1εtau/+) shortened the circadian period. Restoring this mutation in the SCN area somewhat rescued the phenotype53. This study did not specifically describe how astrocytes control the circadian rhythm; however, Brancaccio et al. predicted that it is partially due to a disruption in astrocyte-neuron signaling in the SCN52. Such an interaction also appears to occur in other species, as the astrocyte-specific expression of the circadian factor Ebony rescued circadian behaviors in Drosophila54. These studies suggest that manipulating astrocyte signaling in the SCN can drastically modulate circadian locomotion. It is especially notable that astrocytic circadian control alone is sufficient to drive circadian behavior and that dysfunctional astrocytic circadian control alone is sufficient to break circadian behavior. In both cases, astrocytic outputs completely overshadow the effect of neuronal outputs. These results suggest that astrocytes are not just complementary to neurons but instead are some of the most important players in controlling circadian behaviors.

Beyond circadian locomotive behaviors, several studies have suggested that controlling astrocyte signaling also affects sleeping patterns. Sleep homeostasis is maintained by adenosine accumulation, which is why caffeine, an adenosine receptor antagonist, promotes wakefulness55. The source of this adenosine was assumed to be neuronal. However, several studies have clearly demonstrated that this adenosine comes from astrocytes. Not surprisingly, one of the most well-known gliotransmitters is ATP. Astrocytic exocytosis via the SNARE complex can be disturbed by introducing a dominant-negative version (dnSNARE), resulting in impaired gliotransmission. When astrocytic dnSNARE was systematically expressed in mice, sleep homeostasis was impaired, and cortical electroencephalogram (EEG) recordings showed attenuated slow wave activity following sleep deprivation56. An increase in slow waves is a normal compensatory response to accumulating sleep pressure. The attenuated slow wave power was partially rescued when the A1 receptor antagonist cyclopentyltheophylline was applied intraventricularly, which indicates that purinergic gliotransmission contributes to sleep homeostasis56. Alternatively, when cortical astrocytes were optogenetically stimulated in vivo with channelrhodopsin-2 (ChR2), nearby neurons switched to slow wave oscillations57. Chemogenetic activation also obtained the same result29. It is also notable that astrocytes massively synchronize their Ca2+ activity before neurons drift into slow wave activity58. Another study dampened astrocytic Ca2+ activity to induce slow wave oscillations. Expressing the Venus-IPP fusion protein specifically in astrocytes enhanced IP3 to IP2 metabolism and attenuated agonist-induced Ca2+ signaling in astrocytes59. When this IP3/Ca2+ pathway was impaired in hippocampal astrocytes, mice exhibited elevated slow wave power and increased rapid-eye movement (REM) sleep duration without alterations in their non-REM (NREM) sleep pattern59. In addition, optogenetic stimulation of astrocytes in the hypothalamus at 10 Hz increased both NREM and REM sleep sessions within a 6-h period60. In Drosophila, the astrocytic release of a TNF-α homolog is necessary for a sleep deprivation-induced homeostatic response61. In that study, purinergic gliotransmission was responsible for the accumulation of sleep pressure. Interestingly, two different astrocytic agents drove seemingly opposing effects. Astrocytic glutamate promoted the physiological circadian rhythm, while astrocytic ATP promoted a wakeful state over a sleep state. However, the astrocytic dnSNARE models used in these studies restricts astrocytic gliotransmission globally. Although the in vitro data suggest that the hippocampus is the region responsible for promoting the wake state, this has not been confirmed in vivo. It is also interesting to note that purinergic gliotransmission is also responsible for negative effects that accompany disruption of the circadian cycle, such as impaired learning and memory, which will be further discussed in a later section.

Astrocytes and cognition

Cognition, learning, and memory are some of the biggest mysteries in the field of neuroscience. Although significant progress has been made in understanding how we form and recall memories, numerous aspects of memory are yet to be elucidated. Several studies suggest that astrocytes contribute to this process, and acknowledging their roles would advance our understanding of cognitive processes in general. There is accumulating evidence that astrocytes are critically involved in memory formation through various mechanisms, such as by selectively strengthening synapses. Martin-Fernandez et al. used the endocannabinoid and DREADD systems to chemogenetically manipulate astrocytes in the medial subdivision of the central amygdala (CeM)62. Astrocytes in this region respond to endogenous cannabinoids via astrocytic cannabinoid receptor type 1 (CB1R) to have elevated Ca2+ levels. This then decreases the excitatory input from the basolateral amygdala (BLA) to the CeM62. Astrocytic expression of stimulatory Gq-DREADD hM3D in this area also causes elevated Ca2+ levels and similar neurophysiological changes. Therefore, both endocannabinoids and Gq DREADD activation of CeM astrocytes caused mice to be completely incapable of forming fear memories in a cued fear-conditioning task62. The study also found that elevated Ca2+ levels in astrocytes-triggered ATP release and modulated A1R/A2R activities to selectively enhance inhibitory synapses in the CeM62. In several other studies, an astrocytic dnSNARE mouse model was employed to show that gliotransmitters are involved in memory formation. Regulating astrocytic ATP release with dnSNARE alleviated post-sleep deprivation memory impairment in object recognition tests by rescuing synaptic plasticity in the hippocampus56,63. This implies that astrocytic ATP not only forces the awake state when one is deprived of sleep but also somewhat eliminates the negative consequences of sleep deprivation. Interestingly, these mice did not seem to accumulate sleep pressure, as if they did not require sleep. In addition to ATP, activity-dependent d-serine is absent in the hippocampus in astrocytic dnSNARE mice64. d-serine is an endogenous coagonist of NMDA receptors (NMDARs) at CA3-CA1 synapses. dnSNARE mice performed poorly in contextual fear memory, which was rescued by external d-serine administration64. More recent studies have also demonstrated that astrocytic d-serine release depends on astrocytic CB1 receptor activity. One genetic knockout model (GFAP-CB1-KO) showed reduced NMDAR active-site occupancy, reduced in vivo hippocampal LTP, and impaired object recognition memory65. Along with gene knockout, optogenetic and chemogenetic activation of CA1 astrocytic signaling was also sufficient to enhance memory formation in a context-dependent manner for contextual fear memory and the T-maze66. In that study, Opto-Gq and Gq-DREADD hM3D were employed to demonstrate that astrocytic stimulation is sufficient to promote LTP in the hippocampus. Although neuronal stimulation of the area impaired memory performance, astrocyte stimulation enhanced it. However, astrocytic memory enhancement was situational. When astrocyte stimulation occurred with learning itself, cognitive augmentation occurred66. The effects of NMDAR activity shown in that study imply that d-serine is involved in the de novo potentiation of CA3 to CA1 synapses66. By intervening in astrocyte cell signaling through various methods, multiple studies have found a correlation between astrocyte activity and memory formation. This relationship appears to involve gliotransmission, notably transmission involving ATP and d-serine, in the hippocampus. Multiple studies agree that astrocytic d-serine release occurs in the hippocampus as the Gq pathway is activated. This d-serine critically modulates LTP formation in this area. However, gliotransmission is dysfunctional in some pathophysiological models. In a mouse model of Alzheimer’s disease (AD), astrocytes release excessive GABA through the Best1 channel. The cognitive symptoms exhibited by a mouse model of AD were alleviated by oral administration of selegilline, which blocks monoamine oxidase B activity, ultimately blocking astrocytic GABA synthesis67. Therefore, this study identified GABA as another potential gliomodulator of cognition. In vitro data suggest that hippocampal activities can be rescued by either inhibiting GABA synthesis or blocking the Best1 channel. However, a GABA synthesis inhibitor was not administered in vivo in the above-mentioned study. Therefore, further confirmation of this phenomenon is necessary.

The studies that we have described thus far have concluded that astrocyte signaling results in gliotransmitter release that affects synaptic function. Another interesting hypothesis is that rather than modulating synapses, astrocytes modulate the activity of nearby neurons by supplying them with energy in the form of lactate. The significance of lactate transportation in the CNS was first described in 201168. Since then, multiple groups have investigated the in vivo behavioral role of the lactate transport system in astrocytes. Glycolysis in astrocytes results in lactate release via monocarboxylate transporters (MCTs) 1 and 4 (astrocytes) to MCT2 (neurons). One study found that disruption of the astrocytic lactate transporters MCT1 and MCT4 in the hippocampus caused amnesia68. Adrenergic signaling of the area triggers astrocytic release of lactate, and β2-adrenergic receptors (β2AR) are expressed by hippocampal astrocytes. When exposed to a β2AR antagonist, mice failed to exhibit long-term inhibitory avoidance memory formation tasks69. This effect was rescued when lactate was supplied locally, indicating that these receptors are responsible for learning-evoked lactate release from astrocytes in the region69. In accordance with this result, another study administered a β2AR agonist by injection over multiple days. This intervention activated downstream signaling pathways, including pathways associated with lactate metabolism and transportation, and greatly improved the performance of the animals in the Morris water maze test70. In the hippocampus, astrocyte-neuron lactate transfer is important for the consolidation/reconsolidation of appetitive conditioning. Infusion of glycogen phosphorylase into the BLA of rats impaired astrocyte-neuron lactate transportation. This effect not only prevented rats from forming the new cocaine-based conditioned place preferences but also disturbed existing preferences71. These studies collectively suggest that, in response to external stimuli, astrocytes undergo glycolysis to produce lactate and release it to adjacent neurons. Astrocytic lactate plays a crucial role in forming and recalling memories across multiple brain regions, including the hippocampus and amygdala. Combined with previous descriptions, it appears that hippocampal astrocytes modulate learning on multiple levels. Inputs coming into the hippocampus are sensed by local astrocytes. These inputs activate the Gq pathway and lead to Ca2+ elevation. Astrocytes then oversee lactate transportation and local adrenergic and d-serine signaling, which promote neuronal activity and synaptic plasticity in the area. An increasing amount of in vivo evidence suggests that hippocampal astrocytes are important for learning and memory. This evidence underscores the importance of fully elucidating neuron–astrocyte communication to solve one of the biggest mysteries in neuroscience.

Astrocytes and mood-associated behaviors

Soon after astrocytes were found to react to nearby neuronal activity, they were also seen to respond rapidly to pathophysiological states in the brain. Traditionally, astrocytes were thought to mainly respond to physical damage in the brain, such as ischemia or neurodegenerative diseases (such as AD). Recent advancements in the understanding of astroglial functions suggest that astrocytes are also involved in neuropsychiatric pathophysiology. For instance, astrocytes display altered morphology when neuropathic pain is induced43. Similarly, major depressive disorder is associated with a reduced number and density of astrocytes, and astrocytes exhibit cell hypotrophy in both rodents and humans72. Cao et al.73 showed that astrocytic signaling can have both depressive and anti-depressive effects on mice. In this study, Itpr2/ transgenic mice were used to disrupt the IP3 signaling pathways in astrocytes. Astrocytic Ca2+ elevation and ATP release failed to be induced in knockout mice, but neuronal ATP release was unaffected73. These mice were susceptible to chronic social defeats and rapidly developed depression-like behaviors. Notably, systemic administration of ATP induced antidepressant-like effects in the mice73. In addition, the mice that were previously affected by chronic social defeat stress quickly recovered when astrocytic Gq signaling was enhanced by overexpression of the mas-related gene A1, which elevates intracellular Ca2+. These mice show decreased immobility in a forced swim test and increased interaction during social defeat sessions73. However, there is contradictory evidence regarding whether IP3R2-dependent Ca2+ signaling and gliotransmission induce anxiety or depressive-like behavior74. In the above-mentioned study, IP3R2 KO model mice did not show any abnormalities in the open field test or the tail suspension test. Therefore, given the multiple contradicting results, it remains difficult to clarify the relationship between astrocytic activity and depression. This controversy may be explained by the complex nature of depression and the lack of decisive behavior tests that are available to evaluate it.

In contrast, another mood-associated behavioral disorder is well known to be governed by astrocytes. Obsessive-compulsive disorder (OCD) is an anxiety disorder that is characterized by repetitive behaviors. The most prominent theory regarding the underlying mechanism of OCD is a dysregulated excitation to inhibition (E/I) ratio. As astrocytes are homeostatic controllers, several studies have found that astrocytes are involved in repetitive behavior. Astrocytes express glutamate transporter 1 (GLT1) to regulate the E/I balance. One study found that astrocyte-specific conditional GLT1 knockout mice (GLASTCreERT2/+/GLT1flx/flx) exhibited pathological levels of repetitive behaviors to the point of injuring themselves75. Systematic administration of NMDA antagonist alleviated this symptom, which suggests that astrocytic glutamate transportation via GLT1 plays a critical role in controlling repetitive behaviors75. In another study, a modified isoform of the human plasma membrane calcium pump (hPMCA2w/b) was exogenously expressed specifically in striatal astrocytes45. This calcium pump constitutively extrudes cytosolic Ca2+, which renders cells almost devoid of calcium. Although hPMCA2w/b mice did not display any anxiety or depression-like phenotypes, they demonstrated extensive self-grooming behavior when they were left alone45. As a putative underlying mechanism of such behavioral alterations, in vivo Ca2+ imaging revealed that medium spiny neuron (MSN) activity was significantly dampened when nearby astrocyte Ca2+ signaling was abolished. Blocking the astrocytic GABA transporter GAT-3 also partially rescued the phenotype. This result suggests that astrocytic Ca2+ signaling is responsible for tonic GABA inhibition in the striatum40. Interestingly, GABA release from MSNs results in acute behavioral hyperactivity and disrupted attention, which is rescued by inhibition of the astrocytic GABAB-Gi pathway76. Artificially activating this pathway also upregulates thrombospondin-1 in astrocytes, which in turn increases excitatory synapses and enhances cortical synaptic transmission76. These results suggest that MSNs and astrocytes in the striatum reciprocally exchange GABA signals. Additionally, tonic GABA signals that result from elevated astrocytic Ca2+ inhibit the activity of MSNs to produce repetitive behaviors (Table 1).

Table 1 In vivo manipulation of astrocyte signaling.

Other behaviors

Direct behavior control by astrocytes is not limited to the circadian rhythm, memory, and mood. Multiple studies have controlled various aspects of astrocyte signaling to produce diverse observable behaviors. Just as astrocytes control homeostasis of the circadian rhythm, they also regulate nutritional homeostasis. DREADD-mediated modulation of astrocytes in the arcuate nucleus using Gq-DREADD hM3D altered appetite in mice77,78. The mice demonstrated an acute increase in food consumption following CNO administration. Activated astrocytes also specifically interact with adjacent AgRP/NPY neurons via adenosine A1 receptors to modulate food intake78. Yang et al.77 found the opposite effect to be true when they “inhibited” astrocyte activity using Gi-DREADD hM4D. However, multiple other reports have found that the Gi pathway also results in elevated Ca2+ levels in astrocytes15,28,29. The Gq and Gi pathways result in different degrees of Ca2+ elevation15. However, it remains to be determined whether the Gi pathway truly is “inhibitory” and reverses the effects of the Gq pathway. In addition to feeding behavior, astrocytes are also involved in reward and aversive behavior. We have previously discussed a study showing how endocannabinoid input to astrocytes is necessary for reward-based learning62. Optogenetically stimulating astrocytes in the ventral tegmental area (VTA) with ChR2 resulted in clear, real-time avoidance of the “light-ON” area during a real-time place preference test79. Such avoidance behavior was absent in GLT1-cKOVTA Astrocyte mice, indicating that astrocytic glutamate transport in the area is crucial for modulating aversive behavior79. In that study, astrocytes were shown to adjust the glutamatergic input to local GABA neurons that inhibit dopamine neurons in turn79. Although the brain subregions examined in these studies differed (the amygdala vs. the VTA), they both demonstrate that astrocytic signaling is crucial for the development of healthy approach-avoidance behavior. Another study suggested that proper motor skill learning depends on astrocyte signaling. When astrocyte Ca2+ signaling was attenuated via IP3R2 knockout or the astrocyte-specific metabolic inhibitor fluorocitrate, mice demonstrated poor performance in motor skill learning80. In that study, both IP3R2 deficiency and fluorocitrate treatment impaired LTP-like activity in the motor cortex. This impairment prevented motor skill training-induced glutamate transmission80. These findings indicate that astrocytic Ca2+ is implicated not only in decisive memory formation but also in procedural memory formation.


The diverse behavioral roles of astrocytes in the CNS are becoming more apparent. In the last decade, astrocytes have emerged as critical modulators of information processing in the brain. Although numerous reports have been shifting attention from neurons to astrocytes, the precise in vivo functions of astrocytes have only begun to be decoded. This review discusses recent efforts aimed at elucidating how the astrocyte signaling process is carried out and presents wide-ranging evidence that controlling astrocyte activity can directly affect animal behaviors. Endeavors to decipher the star codings are relatively new, and our current understanding of astrocyte physiology is limited. Although assessing astrocyte function in behaviors in vivo is challenging, new technologies and more accurate models are constantly arising to help researchers precisely monitor and manipulate astrocyte activities in vivo, which will aid in establishing and validating more refined theories of behavioral neuroscience based on neuron–glia communication. The future of research on astrocytes in behavioral neuroscience promises to be fascinating and will likely accompany meaningful real-life applications in fields such as clinical medicine.


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This work was supported by the Samsung Science & Technology Foundation (SSTF-BA150208282).

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Park, K., Lee, S.J. Deciphering the star codings: astrocyte manipulation alters mouse behavior. Exp Mol Med 52, 1028–1038 (2020).

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