Brown adipose tissue (BAT) was thought to disappear after infancy. Recent findings of BAT in patients undergoing positron emission tomography/computed tomography (PET/CT) have renewed the interest in deciphering the relevance of this tissue in humans. Available data suggest that BAT is more prevalent in children than in adults and that its activation during adolescence is associated with significantly lower gains in weight and adiposity. Data also show that pediatric patients with metabolically active BAT on PET/CT examinations have significantly greater muscle volume than patients without identifiable BAT. Both the activity and the amount of BAT increase during puberty. The magnitude of the increase is higher in boys as compared with girls and is closely related to gains in muscle volume. Hence, concurrent with the gains in skeletal muscle during infancy and puberty, all infants and adolescents accumulate large amounts of BAT. These observations are consistent with in vitro investigations suggesting close interactions between brown adipocytes, white adipocytes, and myocytes. In this review, we discuss the potential role of this tissue in regulating weight and musculoskeletal development in children.
It has been nearly five centuries since Konrad Gessner described a tissue that was “neither fat, nor flesh, but something in between (1),” five decades since Robert E. Smith showed the capacity of brown adipose tissue (BAT) to dissipate energy as heat (2), and 5 y since the ranks of obese individuals overtook the number of malnourished individuals in the world (3), but whether BAT has any relevance to human health and disease beyond helping to maintain normal body temperature in newborns remains unknown. The belief that BAT involutes soon after birth and the lack of techniques to adequately measure BAT in humans have limited our understanding of the relevance of this tissue.
New data showing that some adults and most children have BAT, coupled with the development of imaging techniques that provide reliable BAT measurements, has renewed the interest in deciphering the physiology of this tissue (4,5). In this review, we discuss recent advances in our understanding of the potential role of this tissue in regulating weight and musculoskeletal development in children and adolescents.
Anatomy, Physiology, and Pathology of BAT
The adipose organ is a complex endocrine system, composed of white and brown fat. White adipose tissue (WAT) serves as the primary site of energy storage, storing triglycerides within individual adipocytes, whereas BAT stores little fat, burning it instead to produce heat and regulate body temperature (6,7). As compared with WAT, BAT is highly vascularized and innervated by the sympathetic nervous system. Moreover, white adipocytes are spherical and unilocular, whereas the brown adipocyte is usually smaller and characterized by multilocular lipid droplets and an abundance of mitochondria expressing uncoupling protein-1 (UCP1) (7). In humans, BAT is recognized primarily in the cervical–supraclavicular area, where it is present across a wide range of ages (4,5,8,9). To date, the only known function of BAT is to dissipate energy through the uncoupling of oxidative respiration from the production of adenosine triphosphate, an action regulated by UCP1 (7,10,11). This type of heat production is known as nonshivering thermogenesis.
In a thermoneutral state, the activity of UCP1 in the mitochondrial membrane is inhibited by adenosine triphosphate in the cytoplasm. During cold acclimation, sympathetic stimulation triggers the proliferation and differentiation of precursor cells toward the development of BAT (12). Mitochondrial biogenesis and increased synthesis of UCP1 are hallmarks of the thermogenic recruitment process. The release of norepinephrine, associated with a cold environment, interacts with β-adrenergic receptors in the cell membrane of the brown adipocyte, leading to the hydrolysis of triglycerides and the production of free fatty acids (13). The increase in free fatty acids in the cytoplasm overcomes adenosine triphosphate inhibition by interacting with UCP1, leading to its activation. UCP1 increases proton leakage across the inner membrane of brown adipocyte mitochondria, dissipating energy in the form of heat rather than adenosine triphosphate (12).
Besides UCP1, four additional uncoupling proteins are expressed by the human genome. Although UCP1 is exclusive to BAT, UCP2 is expressed ubiquitously, UCP3 is mainly expressed in skeletal muscle, and UCP4 and UCP5 are expressed in the brain (14). The physiological functions of these proteins are not well understood but could have profound significance in our understanding of conditions such as diabetes, obesity, thyroid disease, and aging. Thyroid hormone, an important regulator of energy expenditure, is not only necessary for the full expression of UCP1 but also for UCP3 expression and UCP3-mediated uncoupling in skeletal muscle mitochondria of rodents (14).
There are two rare pathological disorders closely related to BAT. Hibernomas are benign tumors arising from brown adipocytes; hibernomas have primarily been described in the neck, axilla, thoracic regions, and retroperitoneum most commonly in young adults (15). Subcutaneous fat necrosis of the newborn is another clinical condition, often associated with asphyxia or hypothermia, that pathologically is characterized by focal areas of fat necrosis that are infiltrated by macrophages and brown adipocytes at several stages of degeneration (16).
Recent Advances in Pediatric BAT Research
Until recently, the accumulated information on the thermogenic effect of BAT was derived from studies on rodents and hibernating mammals, with little awareness of its potential relevance to humans. Although first described in neonates back in 1902 (17), BAT only began to take on a life of its own a century later with the introduction of positron emission tomography/computed tomography (PET/CT). Today, a large body of evidence indicates that metabolically active BAT is present in a significant number of patients with cancer undergoing PET/CT examinations (4,5,8,10). However, there is a strikingly higher prevalence of BAT in pediatric PET/CT examinations (ranging from 31 to 77%) (4,18,19,20,21,22) as compared with the prevalence in adults (5), ~1 in 2 children vs. 1 in 20 adults.
The depiction of BAT by PET/CT in children, as in adults, is related to environmental temperature and season. BAT activity is observed in most studies during cold exposure (4,23), consistent with histological evidence indicating that BAT is universally present in children and adults (24,25). The percentage of PET/CT studies with metabolically active BAT is also higher in the winter months. This is true even in children living in warmer climates and when studies are obtained under thermoneutral conditions (4,18). Indeed, it is becoming increasingly apparent that photoperiod and day length are strong determinants of BAT activity, independent of environmental temperature (6,26).
Visualization of BAT in children is also dependent on disease status, weight, body composition, and the degree of sexual development (4,18,27,28). Although excessive calorie intake has also been suggested to stimulate BAT activity in mice, whether diet induces BAT activation and thermogenesis in humans is the subject of considerable discussion (29). However, UCP1 polymorphisms have been reported to influence postprandial thermogenesis after a high-fat meal in healthy boys (30).
A recent study in children with lymphoma found that whereas only ~10% of the PET/CT examinations at diagnosis exhibited BAT, ~80% of the follow-up examinations displayed BAT when the patients were in remission (27). Although the mechanism responsible for the suppression of BAT activity is unknown, patients with lymphomas have high circulating levels of tumor necrosis factor-α (31), a pluripotent cytokine reported to elicit apoptotic degeneration of brown adipocytes (32). This cytotoxic effect is known to be mediated by the p55 tumor necrosis factor-α receptor subtype, and its deletion has been shown to increase thermogenesis with an associated increase of UCP1 expression in BAT (33).
In contrast, the visualization of metabolically active BAT in adult patients with cancer is thought to be independent of disease status (34). Unfortunately, the low BAT prevalence, long treatment courses, and relatively poor survival rates greatly hinder longitudinal assessments of BAT in adult populations.
Association of BAT With Weight and Measures of White Adiposity in Children
Data from animal studies indicate that a reduced amount or function of BAT leads to obesity, insulin resistance, and dyslipidemia, whereas an increased amount or function protects against weight gain and its comorbidities (35,36,37,38). Studies in humans also suggest that WAT and BAT are inversely related (5,6,8,11,34). Lean patients exhibit greater BAT activity than obese subjects, and most studies in adult patients report a negative relation between body mass and/or body fat and the degree of metabolically active BAT (5,6,9). Other studies, however, found no relation between BAT and body mass (39,40) or measures of adiposity (40,41). Similar discrepancies are reported in pediatric cross-sectional studies; whereas one observed an inverse relationship between BMI and BAT activity (20), others found no significant differences in the weight, BMI, or measures of subcutaneous adiposity between children with and without functioning BAT (18,23).
The activation of BAT in children has been shown to be related to changes in weight and adiposity (28). Pediatric patients with cancer with no visualization of BAT at diagnoses, but with evidence of BAT activity at follow-up PET/CT studies, gained significantly less weight and subcutaneous and visceral adiposity than those who remained without BAT activity when disease-free. On average, increases in weight and subcutaneous fat were three times greater, and those in visceral fat six times greater, in children who did not demonstrate any BAT activity as compared with children who had metabolically active BAT (28).
The results of a recent study based on magnetic resonance imaging provide additional evidence that overweight/obese children have significantly less total (functional and nonfunctional) BAT in the supraclavicular area than lean, healthy children (H.H. Hu, L. Yin, P.C. Aggabao, T.G. Perkins, J.M. Chia, V. Gilsanz, unpublished data) ( Figure 1 ). Moreover, there appears to be strong inverse relation between magnetic resonance imaging measures of BAT and weight or BMI percentile in overweight/obese children. Of note, the strength of this negative relation is similar to the strength of the positive correlation between values of WAT and these anthropometric measures.
Although BAT activation could decrease WAT as a result of increased energy consumption (8), it is also possible that WAT suppresses BAT function. The white adipocyte is known to produce cytokines and chemokines, such as tumor necrosis factor-α, interleukin-6, and monocyte chemoattractant protein-1, that induce inflammation and could potentially have cytotoxic effects on BAT (42,43). In addition, an increase in BAT could directly lead to an increase in muscle function and energy expenditure, which, in turn, leads to a decrease in adiposity. Studies are needed to establish the direction of causality between BAT activity and WAT accumulation and the degree to which this relationship is mediated by muscle.
Association of BAT With Muscle Development in Children
Recent data show that pediatric patients with metabolically active BAT on PET/CT examinations have significantly greater muscle volume than patients without identifiable BAT (18). On average, boys and girls who exhibit BAT have ~33–50% greater muscle volume than patients who do not exhibit BAT (18). This clinical observation is consistent with information that brown adipocytes and myocytes share many features, including an abundance of mitochondria, energy expenditure via oxidative phosphorylation, and sympathetically mediated adaptive thermogenesis (38,44,45,46). They also express myogenic factors, such as myf5, and may derive from a common lineage in the paraxial mesoderm (46,47). Further support for a muscle–BAT link comes from a landmark investigation indicating that exercise-induced gains in muscle lead to an increase in the amount of cells with a brown fat phenotype (48). This study identifies a new hormone, irisin, as an exercise-induced myokine that allegedly activates the induction of brown adipocytes in WAT depots, a process known as white fat “browning.”
Skeletal musculature increases substantially during puberty. Gains in musculature associated with sexual development closely equal the growth of all other organs, systems, and tissues combined. Concurrently, a higher prevalence and large amounts of BAT are also present during adolescence. Although <20% of PET/CT examinations in prepubertal girls or boys exhibit metabolically active BAT, >75% of such studies in pubertal teenagers display BAT uptake (4). In addition, the volume of BAT increases during puberty in both sexes ( Figure 2 ). The magnitude of the increase is substantially greater during the late stages of sexual development, higher in boys as compared with girls, and closely related to gains in muscle volume (18). Although the reasons for the pubertal increase in BAT are unknown, data suggest that sex steroids and growth hormone have a marked effect on BAT activity (49,50). Of note, past postmortem studies suggest UCP1 activity to be higher in teenagers as compared with neonates (51).
Association of BAT With Skeletal Development in Children
Two recent clinical studies suggest that BAT might be involved in the regulation of bone mass in humans. The first study, in young women with anorexia nervosa, reported a positive relation between BAT and bone density in the axial skeleton, which was independent of disease status and body mass (52). The second study, in children, found the volume of BAT to be positively related to the amount of bone in the appendicular skeleton, a relation that was also independent of known major determinants of bone acquisition, such as height, weight, and gender (53).
The reason(s) for the association between BAT and bone mass are yet to be defined. Available data, however, support a link between BAT and bone formation. The retinoblastoma protein was recently identified as a mesenchymal cell–fate regulator that controlled differentiation into either the brown adipocyte or the osteoblast (54). Several reports have found this key regulator to be capable of both inhibiting adipogenic differentiation and promoting osteoblast maturation (54,55). Studies in animal models also suggest that BAT may be involved in regulating osteoblastogenesis. Heterotrophic ossification modeled by the bone morphogenic protein-2 is known to induce the accumulation of brown adipocytes and subsequently trigger chondrocyte development and bone formation (56). Moreover, mice lacking functional BAT have very low bone mass, reduced osteoblast activity, and increased bone resorption (57).
Regardless of the mechanism by which BAT influences skeletal growth, maintaining optimal bone mass depends on sensing and transducing mechanical loading information derived from muscle contractions (58). Therefore, the possibility exists for muscle to mediate the relationship between BAT and bone development (53). Support for the notion that BAT is crucial to the maintenance of musculoskeletal integrity comes from two physiologic situations characterized by an abundance of BAT in which decreased skeletal loading and locomotion do not result in muscle or bone loss. Large hibernating mammals are remarkably capable of maintaining their muscle and bone mass despite losing a third of their weight and remaining immobile over a period of nearly 7 mo (58,59). Similarly, infancy is a developmental stage associated with rapid increases in muscle and bone mass despite the lack of significant skeletal loading. Studies are needed to determine the degree to which BAT contributes to the maintenance of muscle function in the absence of mechanical strains associated with loading or locomotion.
To date, most studies on the molecular regulation of BAT have been conducted in animal models; these suggest that at least two types of brown adipocytes from distinct lineages exist: those of myoblast origin and those of adipocyte origin (45). Classic brown adipocytes (i.e., “preexisting” brown adipocytes) that reside in the interscapular BAT depot form during the prenatal stage from myoblastic-like myf5-positive precursors and have a gene profile similar to that of skeletal muscle (46) ( Figure 3a ). These myf5-positive cells differentiate into brown adipocytes through the action of the transcriptional regulators PRDM16, peroxisome proliferator-activated receptor-γ, and/or CCAAT/enhancer binding protein-β. In addition, pockets of a second, distinct type of brown adipocyte are found sporadically in the WAT of adult animals that have been exposed to chronic cold or to peroxisome proliferator-activated receptor-γ agonists. Although these inducible brown adipocytes, also known as brown-in-white cells, possess many of the biochemical and morphological characteristics of brown adipocytes, including the presence of multilocular lipid droplets and UCP1 expression (60), they arise from a non-myf5 cell lineage ( Figure 3b ).
There should ultimately be increased scope for studies decoding the transcriptional control of human brown fat development. This is especially pertinent because the gene profile of specialized tissues, such as BAT, has a very different molecular signature in humans as compared with mice (61). Recent data report differences in the response of UCP1 mRNA to hormonal stimulation even between rat and mouse brown adipocytes (62). Emerging questions that must be addressed regarding the biological significance of the two types of brown adipocytes in humans include: What are the molecular or functional differences between the two types of brown adipocytes? Are the molecular signatures of these cells in humans closer to that of myocytes or to that of white adipocytes? How relevant is the inducible brown adipocyte for the control of energy homeostasis as well as for obesity and metabolic disease? Of note are data indicating that preadipocytes isolated from supraclavicular fat in humans aged 35–64 y are capable of differentiating into brown adipocytes in vitro, regardless of PET status (63). Hence, molecular pathways of brown fat development should be intact and can be reactivated in adult humans. In this regard, synthetic peroxisome proliferator-activated receptor-γ ligands such as thiazolidinedione, widely used in drugs to treat type 2 diabetes, can direct white preadipocytes into mature brown adipocytes (64,65,66); this peroxisome proliferator-activated receptor-γ ligand–induced browning effect is reported to be mediated through stabilization of the PRDM16 protein (67).
Recently, we examined the relevance of brown-in-white cells in humans by analyzing the molecular signature of human BAT isolated from infants and adolescents. To our surprise, BAT in children predominantly expressed brown-in-white cell–selective genes rather than preexisting brown fat–selective genes. These data indicate that human BAT possesses molecular signatures that resemble those of beige cells (68).
Obesity has become the leading cause of preventable death. Because lean children appear to have greater BAT activity than obese subjects, there is considerable interest in defining the mechanisms responsible for relations between BAT and body composition. As it also seems that BAT and muscle mass are positively related and higher muscle mass is associated with better insulin sensitivity and lower risk of prediabetes (69), it is imperative that we examine the influence of BAT on metabolic health. Specifically, there is a need to establish whether a deficiency in BAT in early postnatal life permanently increases the risk of obesity and its comorbidities throughout life.
Of equal importance is to determine how the transcriptional and epigenetic regulatory networks that govern human brown adipocytes respond to early stages of human postnatal growth. Postmortem studies indicate that BAT is established in fetuses within the fifth month of gestation (70). At the time of birth, BAT abundance peaks as reflected by the levels of UCP1, before declining over the next 9 mo (51). Studies are needed to examine the degree to which BAT accounts for phenotypic differences among infants and the degree to which BAT is influenced by maternal health and diet, gestational age, birth weight, and feeding practice. For example, current data show that as compared with formula-fed infants, breast-fed infants are leaner and grow more slowly (52). Not only do breast-fed infants grow at different rates during infancy, but breastfeeding appears to have a profound long-term influence on metabolism and disease risk later in life (71). The notion that BAT accounts for the leaner phenotype of breast-fed infants is supported by observations that leptin, ghrelin, adiponectin, resistin, and obestatin, all hormones involved in energy balance regulation, are identified in breast milk (72). Indeed, both leptin and adiponectin concentrations in breast milk have recently been found to influence UCP1 expression in BAT and to negatively correlate with infant body weight (73).
Given that BAT is a highly dynamic and elusive tissue that can exist in a variety of states depending on a wide spectrum of environmental factors, other noninvasive approaches beyond PET/CT are needed to assess the relevance of this tissue in humans. Thermal imaging is a rapid, nonionizing, and acceptable technique that can reliably quantify thermogenesis within the supraclavicular region in humans (74,75). Increases in BAT activity are closely related to a rise in depot temperature. However, the accuracy of these measures decreases in obese subjects because they are influenced by the amount of subcutaneous tissue in the supraclavicular fossa (76).
Magnetic resonance imaging developments will probably be a major driving force in deciphering the relevance of BAT in children. On the basis of the cytological differences in lipid content and degree of vascularization between BAT and WAT, fast magnetic resonance techniques are being developed that provide reliable BAT measures that can be applied even to infants without the need for sedation (77,78) ( Figure 4 ). It should be noted that BAT depots contain a mixture of multilocular brown adipocytes interspersed within unilocular white adipocytes and that no imaging modality currently has sufficient resolution to localize microscopic deposits of brown adipocytes within a mixed cell population.
Finally, emerging evidence indicates circadian rhythms in BAT activity (79). Data in mice using PET/CT show a diurnal rhythm in glucose uptake by BAT (80). Studies are needed to establish the rhythms in clock gene expression in BAT in humans and whether the activity of this tissue increases during the night. Given the close association among exercise-induced muscle function, the release of irisin, and the formation of brown fat–like adipocytes (48), it is essential to investigate the possible correlate to complete the cycle: Does sleep-induced BAT activity promote structural and metabolic changes in skeletal muscle? And which brown adipokine(s) governs this adaptive response? Only then will we be in a position to begin to understand the mechanism(s) by which musculoskeletal development progresses during periods of inactivity.
Brown fat is known to have been present in mammals over 150 million years ago and was considered an evolutionary advantage solely due to its unique ability to enhance survival in cold environments (7,81). However, it is becoming increasingly clear that this tissue may have greater relevance to human health. The main areas of progress in BAT research during the last decade have been (i) the general acceptance that this tissue is present in humans of all ages and is especially abundant during adolescence; (ii) the recognition that BAT may not only dissipate energy in the form of heat but may also be a key determinant of weight and musculoskeletal development during childhood; and (iii) insights into the complex transcriptional controls of brown fat development. Although much more work is needed, it is tempting to think that our challenge for the next decade lies in delineating the molecular regulation of BAT in humans and the crosstalk between brown and white adipocytes and myocytes. Defining the implications that BAT has for early human growth and how it influences health as we age is a most attractive and promising field of research.
Statement of Financial Support
This study was supported by grant R21DK090778-01 (V.G.) of the National Institutes of Health (NIH); NIH/NIDDK grant K25087931 (H.H.H.); USCF Program for Breakthrough Biomedical Research (S.K.); and NIH grant R01DK097441 (S.K.).
Gessner K . Conradi Gesneri medici Tiguine Historiae Animalium Lib I de Quadripedibus uiuparis. History of Animals, Book 1 of Four-Legged Uniparous. Zurich, Switzerland: Froschaver 1551;842.
Smith RE . Thermogenic activity of the hibernating gland in the cold-acclimated rat. Physiologist 1961;4:113.
Lucas L, Rappeport, A, Jack A . Obesity: an even heftier problem. Financial Times, 27 October 2011.
Gilsanz V, Smith ML, Goodarzian F, Kim M, Wren TA, Hu HH . Changes in brown adipose tissue in boys and girls during childhood and puberty. J Pediatr 2012;160:604–609.e1.
Cypess AM, Lehman S, Williams G, et al. Identification and importance of brown adipose tissue in adult humans. N Engl J Med 2009;360:1509–17.
Saito M, Okamatsu-Ogura Y, Matsushita M, et al. High incidence of metabolically active brown adipose tissue in healthy adult humans: effects of cold exposure and adiposity. Diabetes 2009;58:1526–31.
Cannon B, Nedergaard J . Brown adipose tissue: function and physiological significance. Physiol Rev 2004;84:277–359.
van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, et al. Cold-activated brown adipose tissue in healthy men. N Engl J Med 2009;360:1500–8.
Lee P, Greenfield JR, Ho KK, Fulham MJ . A critical appraisal of the prevalence and metabolic significance of brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab 2010;299:E601–6.
Virtanen KA, Lidell ME, Orava J, et al. Functional brown adipose tissue in healthy adults. N Engl J Med 2009;360:1518–25.
Zingaretti MC, Crosta F, Vitali A, et al. The presence of UCP1 demonstrates that metabolically active adipose tissue in the neck of adult humans truly represents brown adipose tissue. FASEB J 2009;23:3113–20.
Klingenspor M . Cold-induced recruitment of brown adipose tissue thermogenesis. Exp Physiol 2003;88:141–8.
Ouellet V, Labbé SM, Blondin DP, et al. Brown adipose tissue oxidative metabolism contributes to energy expenditure during acute cold exposure in humans. J Clin Invest 2012;122:545–52.
Stillway LW . Bioenergetics and oxidative metabolism. In Baynes JW, Dominiczak MH, eds. Medical Biochemistry. Philadelphia: Elsevier Mosby, 2008:106.
Furlong MA, Fanburg-Smith JC, Miettinen M . The morphologic spectrum of hibernoma: a clinicopathologic study of 170 cases. Am J Surg Pathol 2001;25:809–14.
Ichimiya H, Arakawa S, Sato T, et al. Involvement of brown adipose tissue in subcutaneous fat necrosis of the newborn. Dermatology (Basel) 2011;223:207–10.
Hatai S. 1902 Anatomischer Anzeiger. [Annals of Anatomy [online]], 1902;21:369.
Gilsanz V, Chung SA, Jackson H, Dorey FJ, Hu HH . Functional brown adipose tissue is related to muscle volume in children and adolescents. J Pediatr 2011;158:722–6.
Gelfand MJ, O’hara SM, Curtwright LA, Maclean JR . Pre-medication to block [(18)F]FDG uptake in the brown adipose tissue of pediatric and adolescent patients. Pediatr Radiol 2005;35:984–90.
Drubach LA, Palmer EL 3rd, Connolly LP, Baker A, Zurakowski D, Cypess AM . Pediatric brown adipose tissue: detection, epidemiology, and differences from adults. J Pediatr 2011;159:939–44.
Zukotynski KA, Fahey FH, Laffin S, et al. Constant ambient temperature of 24°C significantly reduces FDG uptake by brown adipose tissue in children scanned during the winter. Eur J Nucl Med Mol Imaging 2009;36:602–6.
Truong MT, Erasmus JJ, Munden RF, et al. Focal FDG uptake in mediastinal brown fat mimicking malignancy: a potential pitfall resolved on PET/CT. AJR Am J Roentgenol 2004;183:1127–32.
Garcia CA, Van Nostrand D, Atkins F, et al. Reduction of brown fat 2-deoxy-2-[F-18]fluoro-D-glucose uptake by controlling environmental temperature prior to positron emission tomography scan. Mol Imaging Biol 2006;8:24–9.
Lee P, Zhao JT, Swarbrick MM, et al. High prevalence of brown adipose tissue in adult humans. J Clin Endocrinol Metab 2011;96:2450–5.
Heaton JM . The distribution of brown adipose tissue in the human. J Anat 1972;112(Pt 1):35–9.
Au-Yong IT, Thorn N, Ganatra R, Perkins AC, Symonds ME . Brown adipose tissue and seasonal variation in humans. Diabetes 2009;58:2583–7.
Gilsanz V, Hu HH, Smith ML, et al. The depiction of brown adipose tissue is related to disease status in pediatric patients with lymphoma. AJR Am J Roentgenol 2012;198:909–13.
Chalfant JS, Smith ML, Hu HH, et al. Inverse association between brown adipose tissue activation and white adipose tissue accumulation in successfully treated pediatric malignancy. Am J Clin Nutr 2012;95:1144–9.
Kozak LP . Brown fat and the myth of diet-induced thermogenesis. Cell Metab 2010;11:263–7.
Nagai N, Sakane N, Ueno LM, Hamada T, Moritani T . The -3826 A -->G variant of the uncoupling protein-1 gene diminishes postprandial thermogenesis after a high fat meal in healthy boys. J Clin Endocrinol Metab 2003;88:5661–7.
Warzocha K, Salles G, Bienvenu J, et al. Tumor necrosis factor ligand-receptor system can predict treatment outcome in lymphoma patients. J Clin Oncol 1997;15:499–508.
Nisoli E, Briscini L, Tonello C, De Giuli-Morghen C, Carruba MO . Tumor necrosis factor-alpha induces apoptosis in rat brown adipocytes. Cell Death Differ 1997;4:771–8.
Romanatto T, Roman EA, Arruda AP, et al. Deletion of tumor necrosis factor-alpha receptor 1 (TNFR1) protects against diet-induced obesity by means of increased thermogenesis. J Biol Chem 2009;284:36213–22.
Rousseau C, Bourbouloux E, Campion L, et al. Brown fat in breast cancer patients: analysis of serial (18)F-FDG PET/CT scans. Eur J Nucl Med Mol Imaging 2006;33:785–91.
Feldmann HM, Golozoubova V, Cannon B, Nedergaard J . UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality. Cell Metab 2009;9:203–9.
Cannon B, Nedergaard J . Thermogenesis challenges the adipostat hypothesis for body-weight control. Proc Nutr Soc 2009;68:401–7.
Cederberg A, Grønning LM, Ahrén B, Taskén K, Carlsson P, Enerbäck S . FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia, and diet-induced insulin resistance. Cell 2001;106:563–73.
Hamann A, Flier JS, Lowell BB . Decreased brown fat markedly enhances susceptibility to diet-induced obesity, diabetes, and hyperlipidemia. Endocrinology 1996;137:21–9.
Cohade C, Osman M, Pannu HK, Wahl RL . Uptake in supraclavicular area fat (“USA-Fat”): description on 18F-FDG PET/CT. J Nucl Med 2003;44:170–6.
Sturkenboom MG, Franssen EJ, Berkhof J, Hoekstra OS . Physiological uptake of [18F]fluorodeoxyglucose in the neck and upper chest region: are there predictive characteristics? Nucl Med Commun 2004;25:1109–11.
Yoneshiro T, Aita S, Matsushita M, et al. Brown adipose tissue, whole-body energy expenditure, and thermogenesis in healthy adult men. Obesity (Silver Spring) 2011;19:13–6.
Guilherme A, Virbasius JV, Puri V, Czech MP . Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol 2008;9:367–77.
Cartier A, Lemieux I, Alméras N, Tremblay A, Bergeron J, Després JP . Visceral obesity and plasma glucose-insulin homeostasis: contributions of interleukin-6 and tumor necrosis factor-alpha in men. J Clin Endocrinol Metab 2008;93:1931–8.
Farmer SR . Brown fat and skeletal muscle: unlikely cousins? Cell 2008;134:726–7.
Kajimura S, Seale P, Spiegelman BM . Transcriptional control of brown fat development. Cell Metab 2010;11:257–62.
Seale P, Bjork B, Yang W, et al. PRDM16 controls a brown fat/skeletal muscle switch. Nature 2008;454:961–7.
Caplan AI . Why are MSCs therapeutic? New data: new insight. J Pathol 2009;217:318–24.
Boström P, Wu J, Jedrychowski MP, et al. A PGC1-a-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012;481:463–8.
Rodriguez-Cuenca S, Monjo M, Frontera M, Gianotti M, Proenza AM, Roca P . Sex steroid receptor expression profile in brown adipose tissue. Effects of hormonal status. Cell Physiol Biochem 2007;20:877–86.
Hioki C, Yoshida T, Kogure A, et al. Effects of growth hormone (GH) on mRNA levels of uncoupling proteins 1, 2, and 3 in brown and white adipose tissues and skeletal muscle in obese mice. Horm Metab Res 2004;36:607–13.
Lean ME, James WP, Jennings G, Trayhurn P . Brown adipose tissue uncoupling protein content in human infants, children and adults. Clin Sci 1986;71:291–7.
Bredella MA, Fazeli PK, Freedman LM, et al. Young women with cold-activated brown adipose tissue have higher bone mineral density and lower Pref-1 than women without brown adipose tissue: a study in women with anorexia nervosa, women recovered from anorexia nervosa, and normal-weight women. J Clin Endocrinol Metab 2012;97:E584–90.
Ponrartana S, Aggabao PC, Hu HH, Aldrovandi GM, Wren TA, Gilsanz V . Brown adipose tissue and its relationship to bone structure in pediatric patients. J Clin Endocrinol Metab 2012;97:2693–8.
Calo E, Quintero-Estades JA, Danielian PS, Nedelcu S, Berman SD, Lees JA . Rb regulates fate choice and lineage commitment in vivo. Nature 2010;466:1110–4.
Thomas DM, Carty SA, Piscopo DM, et al. The retinoblastoma protein acts as a transcriptional coactivator required for osteogenic differentiation. Mol Cell 2001;8:303–16.
Olmsted-Davis E, Gannon FH, Ozen M, et al. Hypoxic adipocytes pattern early heterotopic bone formation. Am J Pathol 2007;170:620–32.
Zanotti S, Stadmeyer L, Smerdel-Ramoya A, Durant D, Canalis E . Misexpression of CCAAT/enhancer binding protein beta causes osteopenia. J Endocrinol 2009;201:263–74.
Seger RL, Cross RA, Rosen CJ, et al. Investigating the mechanism for maintaining eucalcemia despite immobility and anuria in the hibernating American black bear (Ursus americanus). Bone 2011;49:1205–12.
Egginton S, Fairney J, Bratcher J . Differential effects of cold exposure on muscle fibre composition and capillary supply in hibernator and non-hibernator rodents. Exp Physiol 2001;86:629–39.
Frontini A, Cinti S . Distribution and development of brown adipocytes in the murine and human adipose organ. Cell Metab 2010;11:253–6.
Svensson PA, Jernås M, Sjöholm K, et al. Gene expression in human brown adipose tissue. Int J Mol Med 2011;27:227–32.
Hernandez A, de Mena RM, Martin E, Obregon MJ . Differences in the response of UCP1 mRNA to hormonal stimulation between rat and mouse primary cultures of brown adipocytes. Cell Physiol Biochem 2011;28:969–80.
Lee P, Swarbrick MM, Zhao JT, Ho KK . Inducible brown adipogenesis of supraclavicular fat in adult humans. Endocrinology 2011;152:3597–602.
Wilson-Fritch L, Nicoloro S, Chouinard M, et al. Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone. J Clin Invest 2004;114:1281–9.
Vernochet C, Peres SB, Davis KE, et al. C/EBPalpha and the corepressors CtBP1 and CtBP2 regulate repression of select visceral white adipose genes during induction of the brown phenotype in white adipocytes by peroxisome proliferator-activated receptor gamma agonists. Mol Cell Biol 2009;29:4714–28.
Petrovic N, Walden TB, Shabalina IG, Timmons JA, Cannon B, Nedergaard J . Chronic peroxisome proliferator-activated receptor gamma (PPARgamma) activation of epididymally derived white adipocyte cultures reveals a population of thermogenically competent, UCP1-containing adipocytes molecularly distinct from classic brown adipocytes. J Biol Chem 2010;285:7153–64.
Ohno H, Shinoda K, Spiegelman BM, Kajimura S . PPARγ agonists induce a white-to-brown fat conversion through stabilization of PRDM16 protein. Cell Metab 2012;15:395–404.
Sharp LZ, Shinoda K, Ohno H, et al. Human BAT possesses molecular signatures that resemble beige/brite cells. PLoS ONE 2012;7:e49452.
Atlantis E, Martin SA, Haren MT, Taylor AW, Wittert GA . Inverse associations between muscle mass, strength, and the metabolic syndrome. Metab Clin Exp 2009;58:1013–22.
Merklin RJ . Growth and distribution of human fetal brown fat. Anat Rec 1974;178:637–45.
Oddy WH . Long-term health outcomes and mechanisms associated with breastfeeding. Expert Rev Pharmacoecon Outcomes Res 2002;2:161–77.
Savino F, Liguori SA, Fissore MF, Oggero R . Breast milk hormones and their protective effect on obesity. Int J Pediatr Endocrinol 2009;2009:327505.
Zhang Y, Matheny M, Zolotukhin S, Tumer N, Scarpace PJ . Regulation of adiponectin and leptin gene expression in white and brown adipose tissues: influence of beta3-adrenergic agonists, retinoic acid, leptin and fasting. Biochim Biophys Acta 2002;1584:115–22.
Lee P, Ho KK, Lee P, Greenfield JR, Ho KK, Greenfield JR . Hot fat in a cool man: infrared thermography and brown adipose tissue. Diabetes Obes Metab 2011;13:92–3.
Rylander E . Age dependent reactions of rectal and skin temperatures of infants during exposure to cold. Acta Paediatr Scand 1972;61:597–605.
Symonds ME, Henderson K, Elvidge L, et al. Thermal imaging to assess age-related changes of skin temperature within the supraclavicular region co-locating with brown adipose tissue in healthy children. J Pediatr 2012;161:892–8.
Hu HH, Tovar JP, Pavlova Z, Smith ML, Gilsanz V . Unequivocal identification of brown adipose tissue in a human infant. J Magn Reson Imaging 2012;35:938–42.
Hu HH, Perkins TG, Chia JM, Gilsanz V . Characterization of human brown adipose tissue of chemical-shift water-fat magnetic resonance imaging. Am J Roentgen, in press.
Zvonic S, Ptitsyn AA, Conrad SA, et al. Characterization of peripheral circadian clocks in adipose tissues. Diabetes 2006;55:962–70.
van der Veen DR, Shao J, Chapman S, Leevy WM, Duffield GE . A diurnal rhythm in glucose uptake in brown adipose tissue revealed by in vivo PET-FDG imaging. Obesity (Silver Spring) 2012;20:1527–9.
Jastroch M, Withers KW, Taudien S, et al. Marsupial uncoupling protein 1 sheds light on the evolution of mammalian nonshivering thermogenesis. Physiol Genomics 2008;32:161–9.
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Gilsanz, V., Hu, H. & Kajimura, S. Relevance of brown adipose tissue in infancy and adolescence. Pediatr Res 73, 3–9 (2013). https://doi.org/10.1038/pr.2012.141
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