Abstract
Objective: To determine the viruses and genotypes circulating in children attending to Braga Hospital(BH) with AGE from January to September 2008*. To compare RV genotype between two consecutive winter seasons (2007 and 2008).
Methods: Prospective study of all childrenā„16 years, attending emergency room with AGE. A questionnaire and stool samples(SS) were collected. Stools were investigated for the presence of adenoviruses(AdV), rotaviruses(RV), noroviruses(NV) and enteroviruses(Echo) and were genotyped by reverse transcriptase-polymerase chain reaction.
Results: SISTER were collected from 207 children with median age of 17 months (1 month;16 years). In 83 (40.1%) SS were isolated viruses (2 coinfection). RV was the most prevalent(18.8%), followed by NV(11.6%). Adenoviruses and enteroviruses were identified in 7.2% and 3.4%, respectively. NV was the most prevalent in July, August and September, RV was the most frequent detected in all other months. Five genotypes of RV were isolated, the most prevalent was G1P[8](43.6%), followed by G9P[8](33.3%). Two genotypes of NV were identify GII.4(95.8%)- GI(4.2%); 2 AdV: AdV1(33.3%)-AdV2(66.6%) and 5 Echo genotypes:6(2),11,13,30,71.
Conclusions: RV was the most frequent isolated virus. G1P[8] was the most prevalent RV type detected. In 2007 G2P[4] was isolated in 77.8% of the samples, whereas in 2008 was present in only 1case. G9P[8] is still the second genotype identify(1,9% vs 33,3%). These results show the variability of RV strains. This was the first study in Portugal with NV in AGE, we saw that NV was the second most frequent agent and it was the most prevalent in the summer months. No enteric genotypes of AdV were identified.
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* Started RVnational surveillance.
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Neiva, F., Afonso, A., Branca, F. et al. 301 Prospective Study of the Etiology of Viral Acute Gastroenteritis with Rv Genotypes in Two Seasons and Noravirus as Second Cause. Pediatr Res 68 (Suppl 1), 155 (2010). https://doi.org/10.1203/00006450-201011001-00301
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DOI: https://doi.org/10.1203/00006450-201011001-00301