Abstract
Background: The function of exogenous pulmonary surfactant depends of an adequate stability of its components, a highly complex mixture of lipids (mainly phospholipids) and two hydrophobic polypeptides, SP-B and SP-C. This composition could become instable with long time surfactant storage. The Butantan Institute (Brazil) produced a new porcine pulmonary surfactant preparation composed mainly of 76% of phosphatidylcholine (30–35% of the phosphatidylcholine is saturated dipalmitoyl phosphatidylcholine), 6–8% of phosphatidyllethanolamine, 6% of phosphatidylinositol + phosphatidylserine, and 4–6% of sphingomyelin, with 5.6% of two hydrophobic polypeptides, SP-B and SP-C. The objective of this study was to evaluate the Butantan exogenous surfactant function one year after its production, using the premature rabbit model.
Methods: 16 New Zealand White premature rabbits were delivered by c-section at 27 days gestation and randomized into two study groups according to the type of surfactant treatment: Butantan surfactant (n = 8, animals treated with Butantan surfactant kept in refrigerator at 4o C for one year), and Curosurf (Farmalab-Chiesi) group (n = 8; surfactant used 4 months after its production). Animals were ventilated with a preset tidal-volume of 8 ml/kg for 15 minutes, using a ventilator-pletismograph system with the following ventilator settings: respiratory rate (RR), 60 cycles/min; FiO2, 0.21; peak inspiratory pressure (PIP) needed to acquire the target tidal-volume ( 8 ml/kg); PEEP, 0 cmH2O; inspiratory and expiratory time, 0.5 seconds. Ventilatory pressure (VP = PIP-PEEP), tidal-volume (TV), and dynamic compliance (DC = TV/VP) were recorded each five minutes until sacrifice. Statistical analysis was performed by t-test. Significance level was set at 0.05.
Results:
Conclusion: The Butantan surfactant showed to be as effective as a newly synthesized Curosurf to treat respiratory distress syndrome in the premature rabbit model, one year after storage at 4°C in refrigerator.
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Precioso, A., Sakae, P., Haddad, L. et al. 223 Effect Of Storage Time in the In Vivo Function of a New Exogenous Pulmonary Surfactant. Pediatr Res 56, 502 (2004). https://doi.org/10.1203/00006450-200409000-00246
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DOI: https://doi.org/10.1203/00006450-200409000-00246