Abstract
Objective: To analyze the effects of GH and CA on skeletal maturation in IUGR rats. Material and Method: 204 Wistar pups were distributed into the following groups: Control (C), Sham-operated (S), IUGR, IUGR+GH, IUGR+CA y IUGR+CA+GH. IUGR was induced by partial uterine vessels ligation at 14th gestational day. GH treatment lasted from 21 to 60 days-of-age (Genotropin® 3.0 mg/kg/day). Group S was only injected with diluent. CA was made at 21 days-of-age. The tibial cartilage thickness was longitudinally measured on Rx at 1, 21, 42, 63 and 84 days and categorized into levels: (L) L1: maximal thickness, L2: 50% of reduction and N3: absence. Percent differences for each level were compared and clustered by simple ligation method (euclidian distance). Results: In all the groups, males and females, N1 was -on average- 100%. In males, at 63 days-of-age, the percent variation of N3 was: 7% (C and IUGR), 9% (IUGR+CA), 18% (IUGR+CA+GH) and 22% (IUGR+GH). At 84 days of age the groups C, IUGR and IUGR+GH showed -on average- 95.7% of N3. At variance, in IUGR+CA, with and without GH, N3 was 74 and 59%, respectively. In females, at 63 days of age, the greatest percentage of N3 belonged to IUGR+GH (59%), followed by IUGR+CA (44%), IUGR (36%), C (25%), and IUGR+CA+GH (16%). At 84 days of age, N3 averaged 98.5%, except IUGR+CA (89%). The cluster analysis at age 63 indicated that IUGR+CA had the most dissimilar pattern, while C-IUGR and IUGR+GH- IUGR+CA+GH were more similar. At age 84 the most dissimilar group was also IUGR+CA, followed by IUGR+GH and IUGR+CA+GH, being IUGR and C more similar. Conclusions: (a) IUGR and GH administration does not affect the maturation rate, (b) CA delays skeletal maturation in both sexes, and (c) GH combined with CA accelerates maturation rate in IUGR males and females.
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Quintero, F., Guimarey, L., Orden, B. et al. Effect of Growth Hormone (GH) and Castration (CA) on Skeletal Maturation in Intrauterine Growth Retarded Rats (IUGR). Pediatr Res 53, 872 (2003). https://doi.org/10.1203/00006450-200305000-00051
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DOI: https://doi.org/10.1203/00006450-200305000-00051