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Rapid Maturation of Gastric Relaxation in Newborn Infants

Pediatric Research volume 50, pages 629632 (2001) | Download Citation

Supported in part by Janssen Research Foundation.

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Abstract

We studied gastric volume, wall compliance, sensory perception, and receptive relaxation during the first postnatal 80 h in 17 healthy term infants, using a computer-driven air pump and simultaneously measuring pressure and volume within a latex balloon placed through the oropharynx into the stomach. To evaluate gastric compliance, we measured pressures while we infused air into the intragastric balloon at different rates (10, 20, and 60 mL/min) in random sequence. In all infants, there was a linear relationship between intragastric pressure and volume to the maximum pressure tested, 30 mm Hg. Gastric compliance ranged from 0.2 mL/mm Hg to 3.8 mL/mm Hg. Different infusion rates had no effect on compliance. We calculated gastric receptive relaxation by measuring the volume needed to maintain a constant pressure of 10 mm Hg within the balloon for 5 min. Gastric receptive relaxation ranged from 0.5 mL/min to 54 mL/min. Gastric compliance and receptive relaxation increased with postnatal age (r = 0.70, p < 0.005;r = 0.79, p < 0.001, respectively) and with number of feedings (r = 0.80, r = 0.88, respectively, both p < 0.001). There was no correlation between weight or type of feeding (breast versus formula) and either gastric compliance or relaxation. In conclusion, these results may explain the small feedings that neonates ingest in the first days of life. During the first 3 postnatal d, the newborn stomach becomes more compliant and develops more receptive relaxation, associated with a larger volume capacity.

Main

In healthy neonates, the volume of feedings in the first days of life is 60–100 mL/kg per day or about 20–40 mL per feeding. After a few days, the healthy neonate ingests approximately 150 mL/kg per day, or about 75 mL per feeding. The relatively low feeding volume during the first hours of life, coinciding with the physiologic diuresis of excess extracellular fluid that accompanies the transition from prenatal to postnatal life, may be caused by one or more factors, including a lack of hunger or thirst because of CNS depression or arousal, changing hormone concentrations associated with parturition, or immaturity of gastric function. Despite the appearance of anatomic features of the digestive tract early in fetal life (1, 2), many aspects of gastrointestinal motility are immature in the neonate (36). In vitro studies demonstrated rapid postnatal changes in the receptors (7, 8) and responses to agonists in rabbit gastric muscle (9, 10). One potential explanation for the small voluntary feeding volumes in the hours immediately after birth is an immaturity of gastric relaxation to accommodate the entry of a meal.

In healthy children and adults, the gastric fundus and body relax as a meal enters, so that there is little increase in intragastric pressures over a wide range of volumes (1113). Azpiroz and Malagelada (12, 13) used a computer-driven air pump to infuse air into a noncompliant balloon placed in the stomach. Pressures increased initially as the stomach started to fill. As the stomach continued to fill, there was a pressure plateau at which continued increases in intragastric volume caused no increase in intragastric pressure over a wide range of volumes. Finally, the pressures begin to rise again, with the subject experiencing a sense of fullness and then pain. The pressure plateau is caused by gastric accommodation to filling, also known as receptive relaxation. Receptive relaxation requires sensory and motor coordination through vagovagal reflexes, with relaxation likely to be mediated by nitric oxide (14) and dopamine (15).

The purposes of this study were to obtain data about the response of the stomach to distention and to identify factors involved in the maturation of gastric relaxation in healthy term newborn infants.

METHODS

The study group included 17 healthy term breast- or bottle-fed (every 4 h) neonates (eight boys), all within the first week of life. Subjects were approved for testing by the attending neonatologist. The protocol was approved by the Harbor-UCLA Medical Center Human Subjects Committee in May 1993, and written informed consent was obtained from the parents. The assessment was timed to begin 3 h after the previous feeding, or 1 h before the next scheduled feeding.

We placed a latex balloon attached to the end of an 8F infant feeding tube through the oropharynx into the stomach. We monitored the heart rate in all patients during the test session. We began testing when heart rate returned to baseline and the infant was comfortable, several minutes after passage of the balloon. Infants were swaddled and held by a nurse during the test session. A computer-driven air pump simultaneously recorded pressure and volume within the balloon to evaluate variables of gastric volume, compliance, and relaxation. To assure subject safety, we set the maximum intragastric pressure at 30 mm Hg. Any increase in intragastric or intraabdominal pressure >30 mm Hg, such as that caused by a retch or a cry, triggered an instantaneous balloon deflation. For comparison, vomiting is associated with intragastric pressures of approximately 300 mm Hg, and normal gastric antral contractions are 80 mm Hg in healthy term infants, and twice that value in adults. Adult volunteers noted an initial perception from the stomach with balloon distending pressures averaging 23 mm Hg, and pain with pressures averaging 26 mm Hg (16). We used computer software to determine intragastric pressure by subtracting the average pressure generated for each volume during three balloon inflations outside the body from pressures obtained at each balloon volume inside the body. For one part of the testing, we infused air into the balloon at different rates (10, 30, and 60 mL/min) in random sequence. During a second phase of the testing, using the same balloon, we set the intragastric pressure at 10 mm Hg, and assessed balloon volume for 5 min. It was our plan to stop the air infusion when the gastric pressure caused a sign of discomfort indicated by a facial grimace and retch, or when we reached the maximal programed intragastric pressure of 30 mm Hg. Each test session lasted approximately 40 min, after which the balloon was deflated and removed with the tube.

We noted sex, age (in hours), number of meals, and the type of feeding (bottle-versus breast-feeding) for each participant. An actual compliance curve was calculated for each neonate. We defined gastric compliance as the ratio of the difference between two volume measurements and the corresponding pressure values. After reviewing the volume-pressure plots for each child, we decided to calculate compliance from volumes obtained when intragastric pressures were 10 and 28 mm Hg. Thus, gastric compliance was defined by the slope of the volume-pressure curve in units of milliliters per millimeter of mercury. In healthy adults, the initial compliance was 55 ± 6 mL/mm Hg (16). Based on adult data (13, 1618), we defined gastric receptive relaxation by the volume needed to maintain a constant pressure of 10 mm Hg within the balloon for 5 min (in milliliters per minute). We used the Mann-Whitney U test with a level of significance of 0.05 to test for sex differences in birth weight, number of meals, type of feeding (breast versus bottle), gastric compliance, and gastric relaxation. To test age and number of meals as predictors of gastric compliance and receptive relaxation, we used correlation analysis and multiple regression analysis. Age was entered first into the equation as a stable characteristic followed by number of meals.

RESULTS

Gastric volumes at the maximal pressure tested, 30 mm Hg, ranged from 38 to 76 mL. In all neonates, there was a linear relationship between intragastric pressure and volume to 30 mm Hg (Fig. 1). Different infusion rates had no effect on the pressure-volume relationship or on discomfort. There were no complications associated with testing, and there were no heart rate increases to the maximal pressure tested, suggesting an absence of discomfort. Gastric compliance ranged from 0.2 to 3.8 mL/mm Hg. When normalized for body surface area, compliance was 32 ± 10 mL·mm Hg−1·m−2 for adults versus 1–17 mL·mm Hg−1·m−2 in the infants. Gastric receptive relaxation ranged from 0.5 to 54 mL/min in the infants. There were no differences at p < 0.05 between boys and girls in age (in hours), weight at birth, number of meals, type of feeding (breast milk versus bottle), gastric compliance, and gastric relaxation. There was a positive correlation between age and gastric compliance (r = 0.70, p < 0.01) and gastric receptive relaxation (r = 0.79, p < 0.001). There was a positive correlation between number of feedings and gastric compliance (r = 0.80, p < 0.001; Fig. 2), and number of feedings and gastric receptive relaxation (r = 0.88, p < 0.001; Fig. 3).

Figure 1
Figure 1

An intragastric pressure-volume plot from a single distention in one newborn. The flat portion of the curve between 0 and 15 mL is an artifact caused by the volume required to open the balloon. Note the linear pressure-volume relationship from 5 mm Hg to the maximal pressure tested, 30 mm Hg. There is no plateau with a 0 slope, as expected in adults.

Figure 2
Figure 2

Relationship between gastric compliance and the number of feedings in neonates. Each neonate had a fixed compliance value (the slope of the volume-pressure relationship was linear) up to 30 mm Hg. There was a positive correlation between number of feedings and gastric compliance (r = 0.80;p < 0.001).

Figure 3
Figure 3

Relationship etween gastric receptive relaxation and the number of feedings in neonates. Gastric receptive relaxation was evaluated by measuring the volume needed to maintain a constant pressure of 10 mm Hg within the balloon for 5 min. There was a positive correlation between number of feedings and receptive relaxation (r = 0.88;p < 0.001).

Age accounted for 48.5% of the variance in gastric compliance (p < 0.05). Above and beyond age, the number of meals accounted for an additional 14.2% of compliance (p < 0.05). Both factors taken together explain 63% of the increase in compliance in these neonates (p < 0.001). A second multiple regression equation was calculated to predict gastric receptive relaxation with age first and number of meals second as independent variables. Age explained 63.1% (p < 0.0001) and number of meals, 15.4% of the variance in gastric receptive relaxation (p < 0.01). Age and number of meals explained a total of 79% of the variance of receptive relaxation for these neonates. The high correlation between compliance and receptive relaxation of 0.87 (p < 0.001) indicates that they mature at a similar rate.

DISCUSSION

Our current data demonstrated that in contrast to adults, the plateau in intragastric pressure as the gastric lumen distends until full is absent in the newborn. In the newborn intragastric pressures increased for every increase in gastric volume. However, even within the first days of life and the first few feedings, there was a change toward developing greater receptive relaxation and compliance in response to distention. Calculated values for both receptive relaxation and compliance increased during the first 3 d of life. Moreover, the volumes required to fill the stomach to the maximal pressure that we allowed more than doubled during this same period.

The clinical consequences of a noncompliant, nonrelaxing stomach are apparent from the natural history of the newborn infant. The first feedings are small in volume. Regurgitation is common. Both of these observations may be related to a noncompliant stomach. Slow gastric emptying because of postprandial duodenal hypomotility might also contribute to small feeding volumes and regurgitation in the first hours after birth in healthy term infants, as it does in preterm infants (19, 20). Both infant age and the number of feedings increased gastric compliance and receptive relaxation, resulting in larger balloon infusion volumes. Statistics demonstrated that increasing age and number of feedings accounted for more than half the measured changes.

There are so many rapid physiologic changes within the first hours of extrauterine life that explaining how a few hours and a few meals alter gastric neuromuscular function can only be speculative at this time. One possible factor is the surge and ebb of stress hormones during and after delivery (21, 22). Accommodation to luminal distention may be diminished in the first hours of life because of the dominance of sympathetic tone over parasympathetic tone (21, 22).

The rapidly changing newborn gut peptide profile, including increases from adult values in gastrin, enteroglucagon, and neurotensin serum concentrations are another potential influence on gastric adaptation (23). From in vitro studies we learned that gastric smooth muscle undergoes rapid time-dependent changes in the maturation of actomyosin (24), calcium channels (25), neurotransmitter receptors (7, 8), and responses to a variety of ligands (9, 10). It is possible that the matrix of connective tissue supporting gastric smooth muscle requires an initial series of stretches before becoming relaxed.

The most likely explanation for rapid postnatal increase in gastric compliance may be the maturation of vagovagal reflexes (26, 27). Gastric receptive relaxation is regulated mainly by inhibitory vagal neurons and nitrergic neurons (14). Dopamine also inhibits gastric tone (15). Perinatal changes in these signals have not yet been explored, but may be a fertile area for investigation.

In adults, early satiety may occur in the absence of disease as a symptom of functional dyspepsia (28). Early satiety caused by functional dyspepsia may be a consequence of one or more of three mechanisms:1) delayed gastric emptying, 2) visceral hyperalgesia, an increased sensitivity of afferent sensory nerves from the stomach to the CNS, and 3) impaired accommodation to distention (18). From our results, impaired accommodation seems a likely explanation for early satiety in neonates. From results of motility testing, delayed gastric emptying also seems a plausible explanation (19, 20). On the basis of our studies, in which 30 mm Hg did not provoke evidence for discomfort, visceral hyperalgesia seems an unlikely cause of early satiety in the neonate.

A group of thought leaders in gastrointestinal sensory testing recently recommended an infinitely compliant balloon for barostat studies (29). We used a latex balloon because it was more compact, easier, and safer to pass through the newborn's oropharynx than the recommended polyethylene balloon. To compensate for the compliance of the latex, we measured the balloon's filling pressures for each volume when outside the body, and subtracted those values from the pressures obtained at corresponding volumes in the stomach to record the intragastric pressure. We used a random sequence of three different distention rates to assess whether rapid or slow distention altered the results. At the infusion rates tested there were no differences, although in adults there are receptors that respond to either slow or fast stretch (30).

These data add to our knowledge of neonatal physiology and provide insight into the mechanisms for early satiety in the first days of life. Further research will be necessary to determine how rapidly adult patterns are attained, and whether there are clinical implications to these findings.

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Acknowledgements

The authors thank Dr. Emeran Mayer for advice on methodology and data analysis. Also, we are indebted to UCLA biomedical engineer John Parker for creating the hardware and software for our computer-driven air pump.

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Author notes

    • Samuel Zangen

    Neonatal Department, SCMCI Children's Hospital, 14 Kaplan St., Petach-Tikva, 49202, Israel.

    • Tsili Zangen

    Current address (T.Z.): GI Institution, SCMCI Children's Hospital, 14 Kaplan St., Petach-Tikva, 49202, Israel.

Affiliations

  1. Pediatric Gastrointestinal Motility Center, Children's Hospital of Orange County, Orange, California 92868, U.S.A.

    • Samuel Zangen
    • , Tsili Zangen
    • , Lenore Schwankovsky
    •  & Paul E Hyman
  2. Children's Hospital of Pittsburgh, Pennsylvania 15213, U.S.A.

    • Carlo Di Lorenzo
  3. Vanderbilt University, Nashville, Tennessee 37232, U.S.A.

    • Howard Mertz

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https://doi.org/10.1203/00006450-200111000-00016