Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

EXPRESSION OF INTERLEUKIN-1 RECEPTOR ANTAGONIST IN RETINA: EFFECTS OF HYPEROXIA AND GLUCOCORTICOID. † 1331

Oxygen is an important factor in pathogenesis of retinopathy of prematurity(ROP). Proinflammatory cytokines are among the factors mediating the hyperoxic damage. Interleukin-1 (IL-1) is a major cytokine participating in inflammatory response. Another cytokine, IL-1 receptor antagonist (IL-1ra) binds to the IL-1 receptors but does not elicit the intracellular signal transduction. Being a specific antagonist to IL-1, IL-1ra is an anti-inflammatory cytokine. The aim of the present study was to investigate whether hyperoxia and/or glucocorticoid affect the growth of the retinal vessels, and the expression of the anti-inflammatory cytokine IL-1ra. Altogether four groups of newborn rabbits were studied during days 3 and 12: group 1. animals were in room air and received placebo (n=6); group 2. animals received dexamethasone (DX, at 1 mg/mg/day during days 3 to 8) (n=6); group 3. animals were kept in 100% O2 during days 3 to 7 and received placebo (n=10); group 4. animals were kept in O2 and received DX (n=10). Day 12 the eyes were studied for retinal vessel length, and for expression of IL-1ra mRNA in the retina using the Northern blot analysis. According to the present results: 1. hyperoxia decreased the length of the retinal vessels by 75%, and increased the incidence of neovascularization (4 of 6 eyes); 2. the glucocorticoid decreased the axial length of the retinal vessels by 64%, increased the tortuosity of the retinal vessels, and did not cause neovascularization; 3. O2 and DX tended potentiate each others actions in vessel growth inhibition; 4. O2 decreased the expression of IL-1ra m-RNA by 70%; 5. DX had no effect on IL-1ra expression, and did not potentiate the suppression of IL-1ra by hyperoxia. According to present results the inhibition of retinal vessel growth by the glucocorticoid was not associated with neovascularization or with altered expression of IL-1ra. The hyperoxia-induced suppression of the retinal anti-inflammatory cytokine, IL-1ra, serves as an evidence that proinflammatory cytokines are involved in the pathogenesis of ROP.

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lawas, P., Hernandez, H., Bry, K. et al. EXPRESSION OF INTERLEUKIN-1 RECEPTOR ANTAGONIST IN RETINA: EFFECTS OF HYPEROXIA AND GLUCOCORTICOID. † 1331. Pediatr Res 39, 224 (1996). https://doi.org/10.1203/00006450-199604001-01354

Download citation

Search

Quick links