Abstract
Few publications have described the activity of surfactant harvested from tracheo-bronchial material of patients with pulmonary diseases using a bubble-surfactometer. We first determined the minimal surface tensions of ExosurfR, AlveofactR and dipalmitoylphosphatidylcholine / phosphatidylglycerol [7:3; mol/mol ] (DPPC/PG), which were 30.3 mN/m, 11.9 mN/m, and 9.5 mN/m, respectively. Then the influence of AlveofactR and DPPC/PG on surfactant preparations of patients with various pulmorary diseases was measured. Surfactant preparations of patients were purified from sputum or tracheo-bronchial aspirates by means of a sodium bromide gradient ultracentrifugation (1). Cystic fibrosis patients showed minimal surface tensions of 41±5 mN/m compared to 21±4 mN/m in other pulmonary diseases. These values were reduced by 50 to 60 % using AlveofactR or DPPC/PG in vitro.
(1) A Method for Reproducible Pulsating Bubble Studies on Surfactant Isolated from Tracheal Aspirates of Infants. Raymondos k, Martin-Carrera I, Bernhard W, C. Acevedo, von der Hardt, H (submitted).
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Martin-Carrera, I., Bernhard, W., Konstantinos, R. et al. 290 SURFACE ADSORPTION AND MINIMAL SURFACE TENSION OF BRONCHIAL SURFACTANT PREPARATIONS WITH AND WITHOUT EXOGENOUS SURFACTANT IN PULMONARY DISEASES. Pediatr Res 36, 51 (1994). https://doi.org/10.1203/00006450-199407000-00290
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DOI: https://doi.org/10.1203/00006450-199407000-00290