Abstract
Lung hyaluronan (HA) concentration has been found to increase in rabbit pups treated with oxygen (Ped Res 1992;32: 635 & 1994;35:280), and in premature monkeys with respiratory distress syndrome (RDS) (Ped Res 1994;35:238-43). The mechanism behind this increase is unknown.
AIM: To determine if surfactant and N-acetylcysteine, used in the treatment of RDS, influence the HA synthesis by lung fibroblasts.
METHOD: Human fetal lung fibroblasts (ECACC WI-38, 30,000 cells/well), were cultivated in Exosurf (colphoscerile palmitate) 1.69-54 mg/ml, Curosurf (porcine surfactant) 10-80 mg/ml, or N-acetylcysteine 5-100 mg/ml for 48 hours. Subsequently, the HA concentration of the supernatant was determined, and calculated as % of the HA concentration obtained with 10% fetal calf serum, i.e. 467±85 ng/ml ( = 100%). RESULT: With Exosurf and Curosurf HA concentrations of 127-154% and 102-132%, respectively, were obtained. HA concentrations increased from 0.2%±0.6 to 101%±44 with increased concentrations of N-acetylcysteine.
CONCLUSION: Surfactant and N-acetylcysteine stimulate HA synthesis by human fetal lung fibroblasts.
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Johnsson, H., Heldin, E., Sedin, G. et al. 102 SURFACTANT AND N-ACETYLCYSTEINE STIMULATE THE HYALURONAN SYNTHESIS BY HUMAN FETAL LUNG FIBROBLASTS. Pediatr Res 36, 19 (1994). https://doi.org/10.1203/00006450-199407000-00102
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DOI: https://doi.org/10.1203/00006450-199407000-00102