Abstract
Growth hormone (GH) 200 ng/ml, IGF-I 50 ng/ml and dehydrotestosterone (DHT) 10 microMol effects on proliferation, proteoglycan (PG) synthesis, IGF-I and GH binding protein (GH-BP) production of cultured HFC (7-12 weeks old) was studied. Single cell suspension was obtained by trypsinization (1% for 45 inin.) concomita were seeded and grown for 7 days when confluence occurred, as well as piles of cells and cartilage noduli. At 7 days a subculture in DCCM-1 w/o serum was used used to test the response to exogenous hormones. Chondrogenic nature of cells was confirmed by histochemical staining, detection of cartilaginous markers by immuno histochemistry for collagen type II and aggrican In case of a limited supply of cells HFC were grown in droplets of hydrated gel (alginic acid beads). The proliferative effect of cells was 65% to GH, 50% to IGF-I and 35% to DHT. Typical dose response curves were obtained with these hormones. While endogenous IGF-I was detected in culture no change in GH-BP levels was noted. Autocrine paracrine response to GH and DHT in FHC is suggested.
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Katchevich, D., Zadik, Z., Barash, A. et al. CULTURED HUMAN FETAL CHONDROCYTES (HFC) TO ASSESS THE BIOLOGICAL ACTIVITY OF HORMONES INVOLVED IN THE GROWTH PROCESS. Pediatr Res 33 (Suppl 5), S51 (1993). https://doi.org/10.1203/00006450-199305001-00290
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DOI: https://doi.org/10.1203/00006450-199305001-00290