Abstract
Urines hGH was determined using E.I.A. commercial kit (NORDITEST) from NOVO NORDISK A/S. Inter-laboratory quality controls were performed in urines samples and standard calibrators: C.V. were between 18.5-29% at low concentrations (about 5 ng/l) and 10-15% for higher concentrations (about 20 ng/l). First morning urine samples were collected for 3 nights in 227 children (122 boys and 105 girls) mean age 9.0 (range 4 to 15 years) using plastic containers after addition of BSA solution (30% w/v). The following groups were studied: normal subjects (A), short stature with GH deficiency after pharmacological tests (B) and without GHD (C), nephrotic syndrome (D), diabetes mellitus (E), obesity (F), growth advance untreated (G) and somatostation treated (H). The results expressed in u-hGH ng/mmol of creatinine, were:
In conclusion, this study confirmed: (1) a decrease of u-hGH in obese patients, (2) in normal subjects no correlation wiih age between 4-15 years, (3) the pharmacological tests demonstrating both partial or total deficiencies in growth hormone secretion are not always ascertained by the hGH urine assay, (4) hGH urine assays are therefore of great interest in the screening of hGH total deficiency.
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Georges, P., Liefooghe, J., Nicola, L. et al. hGH ASSAY IN URINE: PRELIMINARY RESULTS OF A FRENCH MULTI-CENTRIC EVALUATION. Pediatr Res 33 (Suppl 5), S44 (1993). https://doi.org/10.1203/00006450-199305001-00247
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DOI: https://doi.org/10.1203/00006450-199305001-00247