Abstract
Viral infections of the airways have been demonstrated to produce wheezing in infants and children and facilitate enhanced bronchial responsiveness. As an explanation for these effects virus-induced enhancement of mediator release from inflammatory cells has been suggested. The aim of the present study was to verify this hypothesis. Washed human leucocytes (107 cells/ml) were incubated (2 h, 37 C) with different concentrations of influenza A antigen, P3 and RSV (n = 6 and control group). After further incubation (8h, 37 C) basophils were stimulated (40 min, 37 C) with anti-IgE. Histamine was determined by an automated fluorometric technique, cysteinyl-LT's were measured by means of radioimmunoassay.
Results: 1) Preincubation with influenza A virus led to a statistically significant (p<0.0l) enhancement of HR and cystcinyl-LT-synthesis compared to controls. 2) In contrast preincubation with P3 and RSV did not lead to an enhancement of HR and cysteinyl-LT-synthesis.
From our data we conclude that enhancement of mediator release from leucocytes by viral antigens is not a general phenomenon but seems to be limited to specific viruses (i.e. influenza virus A).
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Luck, W., Lau, S., Kreth, H. et al. 4 INFLUENCE OF INFLUENZA A VIRUS, PARAINFLUENZA VIRUS TYPE 3 (P 3) AND RESPIRATORY SYNCITAL VIRUS (RSV) ON HISTAMINE RELEASE (HR) AND CSTEINYL LEUKOTRIENE (CYSTENYL-LT)-SYNTHESIS FROM HUMAN BASOPHILS. Pediatr Res 30, 628 (1991). https://doi.org/10.1203/00006450-199112000-00034
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DOI: https://doi.org/10.1203/00006450-199112000-00034