Abstract
The factors influencing progression of HIV infection from asymptomatic infection to immunodeficiency have only poorly been understood, but cytokines secreted by infected monocytes have received considerable interest.
We have measured Interleukin 1 (IL-1), Interleukin 6 (IL-6), and Tumor Necrosis Factor levels secreted by monocytes of 41 HIV patients of different stages and of 21 HIV negative controls. Monocytes were isolated by Ficoll-Hypaque and adherence, stimulated in vitro with LPS and cytokines secreted measured by bioassay (IL-1, IL-6) or ELISA (TNF).
In vitro experiments testing the influence of immunoglobulins on cytokine secretion revealed a striking reduction of IL-1 and IL-6 secretion in the presence of IgG (10-25 g/l) to 5 - 10 % of the levels secreted by monocytes stimulated in the presence of medium alone.
We found no difference in cytokine levels secreted by monocytes neither between HIV positive patients and negative donors nor between different stages of HIV infection. Monocytes from patients treated by IgG (400 mg/kg once per month) secreted cytokine levels comparable to cells from patients without treatment.
We found that IgG added in vitro inhibits cytokine secretion of monocytes. In patients treated by IgG however no effect on cytokine release could be observed although the progression of AIDS seems to be influenced.
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Peters, A., Jauger, F., Müller, K. et al. 161 CYTOKINE SECRETION OF MONOCYTES IN HIV-INFECTION AND MODULATION BY IMMUNOGLOBULINS. Pediatr Res 28, 304 (1990). https://doi.org/10.1203/00006450-199009000-00185
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DOI: https://doi.org/10.1203/00006450-199009000-00185