Abstract
The immune response of lymphocytes from subjects with hydatid disease (active or operated) was studied by cultivating the mononuclear cells from such patients in the presence of human hydatid fluid and measuring the [3H]thymidine incorporation into the cells. Maximum transformation was observed on the 6th to 7th day of culture at a hydatid cyst protein concentration of 15 μg/ml of culture medium. The stimulation index (SI) of cells from 10 hydatidosis patients was 4.3±1.6 (mean±SD), with a range from 2.1 to 7, whereas in the control cultures it was 1.1±0.3, with a range from 0.5 to 1.5 (p<0.0005). Cocultivation of lymphocytes from hydatidosis patients and from normal subjects with E. granulosus scolices resulted in a greater killing of the scolices by the sensitized than the nonsensltlzed lymphocytes. The diffence was significant after culturing for l to 7 days (p<0.005). Similarly, the viability of the sensitized lymphocytes cocultivated with scolices was significantly lower than that of nonsensitized lymphocytes cultured in parallel under the same conditions (p<0.005). The findings show that sensitized lymphocytes from subjects with active or cured hydatid disease due to E. oranulosus can kill scolices in vitro demonstrating the importance of cellular immunity in the host defense against the parasite.
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Moustouannis, A., Beratis, N. 9 KILLING OF ECHINOCOCCUS GRANULOSUS SCOLICES BY SENSITIZED LYMPHOCYTES. Pediatr Res 28, 278 (1990). https://doi.org/10.1203/00006450-199009000-00033
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DOI: https://doi.org/10.1203/00006450-199009000-00033