Abstract
ABSTRACT: In dissociated whole brain cell cultures from newborn mice, we have previously shown that during glucose deprivation under normoxia, D-β-hydroxybutyrate and oleic acid are increasingly used for energy production. We now asked whether this glucose dependency of the utilization of D-β-hydroxybutyrate and oleic acid as alternate energy fuels is also present after a hypoxic phase. 3- Hydroxy[3-14C]butyrate or [U-14C]oleic acid were added to 7- and 14-d-old cultures and 14CO2-production compared after hypoxia in normal and glucose-deprived conditions. After hypoxia, the ability of the cells 7 d in culture to increase D-β-hydroxybutyrate consumption in response to glucose deprivation is diminished, 14-d-old cells lose this ability. In contrast, after hypoxia, both 7- and 14-d-old cultures maintain or even improve the ability to increase oleate consumption, when glucose is lacking.
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Bossi, E., Kohler, E. & Herschkowitz, N. Utilization of D-β-Hydroxybutyrate and Oleate as Alternate Energy Fuels in Brain Cell Cultures of Newborn Mice after Hypoxia at Different Glucose Concentrations. Pediatr Res 26, 478–481 (1989). https://doi.org/10.1203/00006450-198911000-00023
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DOI: https://doi.org/10.1203/00006450-198911000-00023