Abstract
In the present study antibody dependent cell-mediated cytotoxicity (ADCC) against betalactoglobulin (BLG) was evaluated in infants with cow's Bilk protein intolerance (CMPI) as compared to infants with coellac disease (CD) and healthy controls.
Methods: Sera from patients were tested in an ADCC assay with lymphocytes from healthy adults as effector cells, and as target cells autologous papalnlzed erythrocytes coated with (BLG) using CrCl3. A standard 51Cr release technique was used in the assay. The ADCC-titres were related to the ELISA-tltres for IgG- and IgA-antibodies against BLG.
Patients: CMPI; Late reactions, failure to thrive and villous atrophy n=8(A); Acute reactions, skin- and gastrointestinal symptoms n=6(B); Acute reactions, skin symptoms only, n=8(C) CD; untreated n=9(D); treated n-9(E). Healthy controls n=10(F).
The CMPI-group with gastrointestinal symptoms, irrespective of late or acute typo, showed increased ADCC-titres compared with controls and the CMPI-group with skin symptoms only. The ADCC-titres In CMPI patients with gastrointestinal symptoms were found to correlate significantly with ELISA-titres against BLG for IgG (p<0.001). In contrast CD have low ADCC-activity, despite high antibody levels against BLG. According to the results, ADCC may be a immunopathologic mechanism in gastrointestinal CMPI.
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Saalman, R., Carlsson, B., Fällström, S. et al. 2 ANTIBODY DEPENDENT CELL-MEDIATED CYTOTOXIC ACTIVITY IN INFANTS WITH COW'S MILK PROTEIN INTOLERANCE. Pediatr Res 24, 405 (1988). https://doi.org/10.1203/00006450-198809000-00025
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DOI: https://doi.org/10.1203/00006450-198809000-00025