Abstract
The role of anti-neutrophil antibodies was investigated as a cause of functional abnormalities of circulating neutrophils in a child with persistent neutropenia and recurrent mouth sores. The patient's neutrophils were isolated by isopycnic centrifugation and compared with normal adult neutrophils. Functional abnormalities in the patient's PMNS included (1) decreased chemotaxis studied by under agarose method and by skin window, (2) decreased oxidative burst measured by luminol amplified chemi luminescence, and (3) decreased superoxide production measured by cytochrome C reduction assay. The ability of the patient's serum to bind normal neutrophils was determined by immunofluorescence. While the patient's serum bound normal PMNs, this binding did not interfere with their oxidative burst. In contrast, the patient's serum did not bind to his own PMNs in vitro even though his neutrophil specific antibody was anti-NAI and his PMNs were NAI positive. Therefore, the functional abnormalities of this patient's PMNs were not due to neutrophil antibody binding alone, but due to a subpopulation of dysfunctional neutrophils in circulation possibly selected out by their low affinity to the circulating autoantibodies.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Belani, K., Clay, M., Herron, J. et al. NEUTROPHIL DYSFUNCTION IN AUTOIMMUNE NEUTROPENIA. Pediatr Res 21 (Suppl 4), 308 (1987). https://doi.org/10.1203/00006450-198704010-00847
Issue Date:
DOI: https://doi.org/10.1203/00006450-198704010-00847