Abstract
In diarrheal diseases with accompanying carbohydrate malabsorption, the unabsorbed carbohydrates and products of bacterial fermentation results in osmotic diarrhea and alterations in transit time. It is unclear whether the loss of this nutrient is mediated by alterations in mucosal hydrolytic enzymes and/or the transport carriers. Adult rats were force-fed by hyperosmotic solution of 20% Mannitol (1300 Mosm.) at a dose of 5 ml/100g. for seven days; a similar group of control rats were treated similarly with plain water. After an overnight fast, the animals were anesthesized with Innovar (2.0cc/Kg.). All carbohydrates were infused by intraduodenal bolus at a dose of 0.5g/Kg. Portal venous blood glucose was assayed at intervals (0, 30, 60, 90, and 120 min.). The areas under the glucose curves were used to compare groups; in the mannitol group the glucose absorption was diminished by 9%, maltose by 20% and glucose polymer (D.E. 24) by 12%, when they were compared with the control group. These data suggest that when the intestinal mucosa is exposed to hyperosmolar loads, digestion of carbohydrate is suppressed more than is its absorption. This correlates well with the decreased disaccharidase activity found in the animals treated with mannitol. The rise in portal venous blood in both groups was blocked by phlorizin (10−2M) administration which demonstrates that the transport of glucose as measured is carrier mediated and not related to alterations in mucosal permeability in the mannitol group.
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Palacios, M., Madariaga, H., Heitlinger, L. et al. DIARRHEA INDUCED BY HYPERTONIC MANNITOL AFFECTS CARBOHYDRATE HYDROLYSIS BUT NOT TRANSPORT. Pediatr Res 21 (Suppl 4), 274 (1987). https://doi.org/10.1203/00006450-198704010-00642
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DOI: https://doi.org/10.1203/00006450-198704010-00642
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