Abstract
The role of insulin (IN) on cerebral glucose(glu) metabolism in newborn mammals is unknown. We employed the euglycemic hyperinsulinemic (HI) clamp (N=36) alone or combined with tissue 2-deoxy [14C] glucose (2DG) uptake (N=21) to see the effects of HI (33-14, 333 uU/ml) on cerebral metabolites and 2DG uptake. Control (N=14) vs HI, basal glu (3.1±1.4 v 3.7 ± 1.1 mM) basal glu utilization (33±8 v 31±12 umol/kg/min) were similar. During HI, glu levels did not change (3.7 v 3.8 mM), however glu utilization increased to 54±28, p<0.005. Compared to fasting values brain metabolites were not changed by HI: glu (1.7±0.9 v 2.0±1.0 umol/g), UDP-glu (0.067±0.026 v 0.069±0.035), glycogen(0.92±0.47 v 1.1±0.69), pyruvate(0.12±0.05 v 0.12±0.05), lactate(0.99±0.52 v 0.93±0.41), citrate(0.21±0.05 v 0.21±0.08). Furthermore, glu-6-P, fructose-6-P, phosphoenolpyruvate alanine, glutamine glutamate, aspartate and ATP were not different. The uptake of 2DG was analyzed as a function of plasma insulin levels (120-6000uU/ml) in brain and also compared with more insulin sensitive tissues; muscle and liver: Mean±S.D.
Conclusion: Although HI did not affect brain metabolites, there was a significant dose response of brain 2DG uptake as a function of increasing insulin levels. Neonatal canine brain may be an insulin sensitive tissue.
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Trindade, C., Huang, M., Hulman, S. et al. THE EFFECT OF EUGLYCEMIC HYPERINSULINEMIA ON CEREBRAL GLUCOSE METABOLISM IN NEWBORN BEAGLES. Pediatr Res 21 (Suppl 4), 223 (1987). https://doi.org/10.1203/00006450-198704010-00339
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DOI: https://doi.org/10.1203/00006450-198704010-00339