Abstract
CALC is the principal failure mode of BHV fabricated from glutaraldehyde pretreated porcine aortic valves or bovine pericardium. Covalent binding of APD to residual glutaraldehyde in pericardial BHV was studied as an approach for the inhibition of CALC. BHV were preincubated in APD (0.14M) at various pH's with and without sodium borohydride (NaBH4) reduction. The bound APD was determined using C14 APD and the CALC was studied with 21 day rat subdermal implants of BHV. APD incorporation was pH dependent with more rapid uptake at higher pH (Table). APD completely inhibited CALC when binding was >30ng/mg, while controls exhibited CALC comparable to several years of clinical implantation. NaBH4 reduction significantly stabilized (p<.001) APD bound to BHV subjected to in vitro incubations (pH 7.4, 37C). In conclusion, APD pretreatment of BHV significantly inhibits CALC and may also be useful with controlled release drug delivery to prevent CALC. Our data suggest that incubation in APD at pH 11.0 for 1 hour during surgical preparation for BHV implant may be all the pretreatment that is necessary to prevent BHV CALC.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Webb, C., Benedict, J., Linden, J. et al. INHIBITION OF BIOPROSTHETIC (BHV) CALCIFICATION (CALL) WITH COVALENTLY BOUND AMINOPROPANEHYDROXYDIPHOSPHONATE (APD). Pediatr Res 21 (Suppl 4), 196 (1987). https://doi.org/10.1203/00006450-198704010-00177
Issue Date:
DOI: https://doi.org/10.1203/00006450-198704010-00177