Abstract
Nitrofurantoin induced pulmonary fibroais may result from free radical(FR) production and subsequent lipid peroxidation(LPO). Furazolidone(FZ), a nitrofuran antibiotic, causes a dilated cardiomyopathy(CM) in turlceys(TK); however, the mechanism of this CM is unknown. Our study was designed to determine if FR injury contributed to this CM. 40 TK were fed FZ (0.5mg/g of feed) starting at hatch. 16 survivors were evaluated by 2D echocardiography and blood presaure(BP) measurements at 5-8 wks. 5 birds with the most severe cardiac dilatation and left ventricular(LV) dysfunction were selected for study and sacrificed by decapitation. LV homogenates(with 1mM deferoxamine) were assessed for LPO by 2 methods: 1) The thiobarbituric acid reaction - malondialdehyde(MDA) production, nmol MDA/100mg prot; 2) Lipid hydroperoxide(HP), nmol/100mg prot. LV superoxlde dismutase(SOD, nitrite units/g wet wt) was also measured(Table). Systemic BP in FZ birds was reduced compared to control(CON) birds(72±12 vs 153±5mmHg, p<0.001).
LV LPO was reduced in the FZ birds with severe cardiac dilation. SOD levels were similar in CON and FZ birds. In a separate study in which FZ was added to LV homogenatea in vitro (2-10mg/g wet wt), FZ did not increase MDA production(data not shown). Thus, although chronic FZ administration produced severe cardiac dilation and dysfunction, our data does not support FR damage as a mechanism for this CM. In fact, reduced LV LPO in FZ CM could be consistent with a protective mechanism.
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Lax, D., Zhang, SL., Li, Y. et al. REDUCED HEART LIPID PEROXIDATION IN DILATED CARDIOMYOPATHY. Pediatr Res 21 (Suppl 4), 191 (1987). https://doi.org/10.1203/00006450-198704010-00150
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DOI: https://doi.org/10.1203/00006450-198704010-00150