Abstract
Subfraction 2R of fraction 9 of a wheat gliadin's peptic-tryptic-pancreatic digest is known to be toxic in vivo for coeliacs (H.J.Cornell et al., Clin.Chim. Acta 31, 123, 1982). We have found that fractions 9 and 2R agglutinate K 562(S) cells and inhibit the in vitro development of fetal rat intestine and the increase of enterocyte height occurring in organ culture of atrophic coeliac mucosa (0.1-0.5 mg/ml medium). Other peptide fractions of the gliadin digest are devoid of such in vitro effects. Fraction 2R, after incubation with morphologically normal small intestinal mucosa of coeliacs in remission and ultrafiltration, was still able to agglutinate K cells and was very active in both culture systems, at low concentration (0.1 mg/ml); on the contrary, fraction 2R was inactivated after incubation with normal mucosa. In intestinal mucosa of coeliacs in remission either a primary (or secondary) enzyme deficiency or some other mechanisms may explain these results which are compatible with the hypothesis that there is a mucosal defect in handling gliadin peptides in coeliac disease.
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Cornell, H., Auricchio, S., De Ritis, G. et al. 37. INTESTINAL MUCOSA OF COELIACS IN REMISSION IS UNABLE TO REMOVE TOXI-CITY OF GLIADIN PEPTIDES ON IN VITRO DEVELOPING FETAL RAT INTESTINE AND CULTURED ATROPHIC COELIAC MUCOSA. Pediatr Res 22, 102 (1987). https://doi.org/10.1203/00006450-198707000-00058
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DOI: https://doi.org/10.1203/00006450-198707000-00058