Abstract
Studies have shown that PMN from adults and neonates exhibit heterogeneity of function and 31D8 MAb binding. The heterogenity of 31D8 binding correlates with PMN motile heterogeneity. MOI MAb binds to PMN glycoproteins which allow the PMN to adhere and move normally. We further studied PMN heterogeneity in 10 neonates and 10 adults by placing PMN in the upper compartment of chemotactic chambers and allowing them to transverse 10 μm polycarbonate filters in response to C5 frag. PMN were analyzed for viability, band count and MAb binding with 31D8 and MO1. The % of non-motile (NM) neonatal and adult PMN recovered from the upper compartment after 90 min. were 7+1% and 4 ± 1%, respectively (P<0.01). The % highly motile (HM) neonatal and adult PMN from the lower compartment were 5 ± 2% and 11 ± 2%, respectively (P<0.01). The viability and % bands in NM PMN were not significantly different from control PMN. The NM PMN had significantly less 31D8 and MO1 MAb binding than the HM PMN sub-populations. Neonatal NM and HM PMN had significantly less 31D8 and MO1 MAb binding than adult NM and HM PMN. These data suggest that variable 31D8 and MO1 antigen expression may contribute to differences in motile subpopulations of PMN in neonates and adults and are at least in part responsible for differences between neonatal and adult PMN motility. Decreased PMN chemotaxis in neonates may also result from a larger subpopulation of NM PMN and a smaller subpopulation of HM PMN.
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Krause, P., Bannon, P., Eisenfeld, L. et al. HETEROGENEITY OF NEUTROPHIL (PMN) FUNCTION AND MONOCLONAL ANTIBODY (MAb) BINDING IN NEONATES AND ADULTS. Pediatr Res 21 (Suppl 4), 328 (1987). https://doi.org/10.1203/00006450-198704010-00964
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DOI: https://doi.org/10.1203/00006450-198704010-00964