Abstract
Periodic intramuscular injection of LRH-M has been shown to suppress pituitary-gonadal axis in children with precocious puberty (Roger et al. ESPE 1984). In order to assess the effects of LRH-M on spermatogenesis, 50 μg/kg were given to Fox dogs on days 1 and 21, then every 28 days. In the adult dogs treated for 15 weeks spermatogenesis was inhibited during therapy and recovered 90 days after the last injection. A one-year treatment was started in 3 adolescent dogs just before completion of puberty. LH level (ng/ml, x̄±sem) decreased from 4.6 ± 0.6 to 1.2 ± 0.1 on day 21 (p<0.05) and became undetectable beyond the 5th injection. Normal or high levels were recovered 60 days after the last injection. Mean testosterone level (ng/ml) was 1.6 ± 0.2 initially, undetectable beyond the 5th injection and 1.3 ± 0.3 sixty days after the last injection. Histology of the right testis on day 371 showed spermatogonia in all tubes, spermatocytes 1 in 5 to 57% of tubes and absence of more mature cells. Initially, no spermatozoa were present in ejaculates, but in 2 dogs sperm cells were present from days 56 to 77 and 64 to 105 respectively, disappeared thereafter and reappared 92 days after the last injection. No sperm cells were found in the 3rd dog throughout the study. This work shows that LRH-M induces a regression of spermatogenesis in adolescent dogs and that recovery occurs after treatment.
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Lahlou, N., Cavitte, J., Mialot, M. et al. SPERMATOGENESIS IN ADOLESCENT DOGS TREATED FOR ONE YEAR WITH D-TRP-6-LH-RH IN MICROCAPSULES (LRH-M). Pediatr Res 20, 1198 (1986). https://doi.org/10.1203/00006450-198611000-00146
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DOI: https://doi.org/10.1203/00006450-198611000-00146