Abstract
Accumulation of adenosine is detected in plasma of ADA-deficient children(1). We incubated human lymphoid cell lines with adenosine in presence of the ADA inhibitor deoxycoformycin. The malignant T cell line Moltland the non-malignant B cell line RPMI 1788 were both more affected by adenosine than the malignant B cell line RAJI was. However, adenosine had a remarkable biphasic effect on cell growth of all three cell lines. After 48 hours incubation with 40μM adenosine growth of all three cell lines was severely inhibited (Molt 493%, RPMI 1788 87%, RAJI 63%). When 100μM adenosine was used the inhibitory effect was less severe (Moltμ58%, RPMI 1788 72%, RAJI 49%). This biphasic effect was also reflected in the cell cycle. After 24 hours incubation addition of 20μM adenosine to the cell cultures resulted in accumulation of cells in the G1 phase while addition of 50μM adenosine resulted in a decrease of cells in the G1 phase. Drawing conclusions about the inhibitory effects of adenosine should be done with caution.
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Van Der Kraan, P., Van Zandvoort, P., De Abreu, R. et al. BIPHASIC EFFECT OF ADENOSINE ON CELL GROWTH AND CELL CYCLE OF HUMAN LYMPHOID CELL LINES: 219. Pediatr Res 19, 780 (1985). https://doi.org/10.1203/00006450-198507000-00239
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DOI: https://doi.org/10.1203/00006450-198507000-00239