Abstract
The metabolism of purine nucleotides has been Studied in the liver and in kidney of adult male Wistar rats before and after castration.The overall rate of purine biosynthesis was determined by following the incorporation of formate- 14C into acid-soluble cellular purines as well as those incorporated into RNA (1).After castration, the level of acid-soluble purines remains the same both in the liver and in the kidney.The specific activity of the bases is only slightly enhanced in the kidney and significantly increased (p<0.05) in the hypoxanthine and in the guanine of the liver,indicating an accelerated turnover. The G/A labeling ratio (counts per min in guanine/counts per min in adenine),which is an index of the "IMP branch point regulation",is slightly augmented in both tissues. The RNA content is unchanged, both in the liver and in the kidney, but the formate- 14C incorporation is enhanced in liver RNA and decreases in kidney RNA.Results indicate that the testosterone deficiency plays different and discordant roles in purine nucleotide metabolism in the kidney and in the liver.
1) M.M.Welch, F.B.Rudolph J.Biol.Chem. 257, 22, 13253, 1982.
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Pizzichini, M., Stefano, A., Matteucci, G. et al. METABOLISM OF PURINE NUCLEOTIDE IN THE LIVER AND IN THE KIDNEY OF CASTRATED RATS: 161. Pediatr Res 19, 770 (1985). https://doi.org/10.1203/00006450-198507000-00181
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DOI: https://doi.org/10.1203/00006450-198507000-00181