Abstract
Procedures for the HPLC analysis of nucleosides and bases have generally been designed to fulfil specific requirements. No single method exists capable of separating all the purines and pyrimidines found in the biological fluids of patients in the different inherited disorders in a single run and within a reasonable time. Because of a need for such a system an appropriate method has been devised.
The system has been in continuous use for over two years and three to four thousand samples have been analysed. It has proved reliable and reproducible throughout the life of many columns. Problems encountered during this period have resulted from artifacts and errors inherent to different isolation/extraction procedures. Pitfalls frequently noted have in the main been due to diet and/or drug metabolites with similar chromatographic behaviour to endogenous purines and pyrimidines.These problems are exaggerated in renal failure and during antibiotic therapy.
Data derived from the chromatography of body fluids of patients with various purine and pyrimidine defects,illustrate the adaptability of the system. The method is particularly applicable to the separation of endogenously derived adenosine based compounds. An abbreviated form of the system has been used successfully for enzyme assays and other in vitro studies.
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Morris, G., Simmonds, H. A SINGLE SYSTEM FOR THE EVALUATION OF PURINE AND PYRIMIDINE NUCLEOSIDES AND BASES TOGETHER WITH THEIR ANALOGUES IN BIOLOGICAL FLUIDS: 137. Pediatr Res 19, 766 (1985). https://doi.org/10.1203/00006450-198507000-00157
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DOI: https://doi.org/10.1203/00006450-198507000-00157