Abstract
Immunofluorescent-labeled glial cells are shown by radioautography to metabolically cooperate with fibroblasts. The observations of cooperation without cell contact and of incorporation gradients suggest that cooperation occurs through extracellular transport of radiolabeled adenine, adenosine or methylthioadenosine. The transfer of these purine compounds is supported by the quantitative loss of cooperation when the recipient cell is also deficient in enzymes required for adenine or adenosine salvage. The demonstration of glial cell cooperation provides impetus for current research toward gene replacement therapy for the neurologic symptoms of certain inborn errors of metabolism, such as Lesch-Nyhan syndrome.
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Koenker, R., Luchtman, L., Willis, R. et al. GLIAL CELLS METABOLICALLY COOPERATE: A POTENIAL REQUIREMENT FOR GENE REPLACEMENT THERAPY: 102. Pediatr Res 19, 760 (1985). https://doi.org/10.1203/00006450-198507000-00122
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DOI: https://doi.org/10.1203/00006450-198507000-00122