Abstract
In addition to the well studied 5′-nucleotidase other enzymes of nucleotide metabolism are characterized on the surface of human lymphoid cells, especially on lymphoblastoid B-cell-lines: An ATPase, an ADPase and a nucleosidediphosphate kinase. The true ectoenzyme nature of all three activities is established by various independent criteria. Typical properties such as substrate specificities, metal ion requirement and kinetic constants are determined. Treatment of intact lymphoblasts with sublytic concentrations of certain detergents induces membrane shedding. All three enzymes together with 5′-nucleotidase are enriched in the shed membrane fraction. All these ectoenzymes seem to follow a pattern of coordinated expression in lymphoblastoid B-cell-lines, with low or absent activities in lines derived directly from Burkitt's lymphoma and high activities in lines established by EBV-infection of normal PBL. On the other hand ATPase, compared to 5′-nucleotidase is much less suitable as an enzyme marker for differential diagnosis in acute leukemias.
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Gutensohn, W., Rieger, J. ECTOENZYMES OF NUCLEOTIDE METABOLISM ON HUMAN LYMPHOID CELLS: 78. Pediatr Res 19, 756 (1985). https://doi.org/10.1203/00006450-198507000-00098
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DOI: https://doi.org/10.1203/00006450-198507000-00098