Abstract
After immunization of mice with partially purified human placental 5′-nucleotidase hybrid cells were produced following the standard protocol. Culture supernatants were screened for the ability to inhibit enzyme activity. From 18 different hybrids showing significant inhibition 5 were established as clones. Properties of the MABs were studied in ascites fluid and in purified Ig-preparations. Inhibition of the MABs is directed against the enzyme in membrane bound and solubilized form and there is no tissue specificity. Similar inhibitory titers were observed with the MABs coated on plastic. The MABs belong to the IgG 1 and IgG 2b subclasses. Some of them show a marked temperature dependence in their inhibitory potency. They are not directed against the sugar moiety of the 5′-N molecule. In fluorescence studies with PBL a smaller subpopulation, compared to polyclonal antisera, shows a positive reaction with the MABs. The titers for optimal fluorescence are much lower than for inhibition. Results of flow-cytometric studies relating cell size to positive reaction with the MABs will be reported.
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Gutensohn, W., Kummer, U. & Forstpointner, R. PRODUCTION AND PROPERTIES OF MONOCLONAL ANTIBODIES AGAINST HUMAN ECTO-5′-NUCLEOTIDASE: 77. Pediatr Res 19, 756 (1985). https://doi.org/10.1203/00006450-198507000-00097
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DOI: https://doi.org/10.1203/00006450-198507000-00097