Abstract
A blood spot (BS) assay was developed for use in infants to supplement plasma/saliva 17P assays used to monitor older CAH children. 17P recovery from a 6 mm blood disc immersed in ethenol, eluted with buffer and solvent extracted was >95%. A magnetised solid-phase antiserum and 125I-tracer was used as in the routine 17P assay. Sensitivity was 1.5 pg/tube (0.5 nmol/L). Simultaneous plasma, saliva and BS samples were collected 1-2 hrly for 12-24hr from treated patients. Profiles of 17P in all 3 matrices was identical, whatever the control (r=0.99). Weekend profiles of BS and saliva 17P in samples collected at home are shown in the Table (means of 2 daily profiles).
BS and saliva 17P values indicated the degree of control, identified over-treatment and again showed a diurnal rhythm in the under-treated. Selective use of plasma, BS and saliva 17P measurement now permits detailed monitoring of CAH at all ages.
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Hughes, I., Robinson, J., Dyas, J. et al. 118 CONGENITAL ADRENAL HYPERPLASIA (CAH) MONITORED BY SERIAL 170H-PROGESTERONE (17P) DETERMINED IN 3 MATRICES. Pediatr Res 19, 623 (1985). https://doi.org/10.1203/00006450-198506000-00138
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DOI: https://doi.org/10.1203/00006450-198506000-00138