Abstract
The study was designed to investigate the effects of bilirubin on synaptosomes from rat brain. Ten percent suspensions of synaptosomes derived from hippocampus and striatum from male Sprague-Dawley rats were prepared in 0.25 M sucrose. A Krebs-Ringer buffer, pH 7.3, gassed with 95% O2, 5% CO2, was used throughout. 75μl of synaptosome suspension ana 625μl of a buffered bilirubin/ albumin solution (molar ratio 8:1) were incubated for 5 min. at 37°C, after which 40μl of 3H-dopamine and 14C-5HT were added and incubation continued for another 10 min. The following bilirubin concentrations (μM) were studied: 1 - 10 - 20 - 40 - 80 - 160 - 320 - 640. The reaction was stopped and the synaptosomes harvested by filtering through a glass microfibre filter premoistened with ice cold saline. Activities were counted in Insta-gel II in a Packard Tri-carb 460 CD scintillation counter. A significant difference from control values (p<0.05) was found starting at the following bilirubin concentrations: In striatum with 5-HT: 80μM, with dopamine: 20μM; in hippocampus with 5-HT: 20μM, with dopamine: 10μ. The results Indicate that bilirubin inhibits uptake of dopamine and 5-HT both in striatal and hippocampal synaptosomes.
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Hansen, T., Tydal, T., Jorgensen, H. et al. 1679 THE EFFECT OF BILIRUBIN ON THE UPTAKE OF 5-HT AND DOPAMINE IN RAT BRAIN SYNAPTOSOMES. Pediatr Res 19, 390 (1985). https://doi.org/10.1203/00006450-198504000-01703
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DOI: https://doi.org/10.1203/00006450-198504000-01703