Abstract
CMV-seronegative and frozen-deglycerolized blood, when used to prevent PTCMV, have disadvantages of increased cost and limited availability. CMV harbored within leukocytes is the presumed source of PTCMV. With the IBM 2991 Blood Cell Processor, we achieved 89% reduction in white blood cells using a protocol of long spins (2.5 to 5 min) and slow superout rate (200 ml/min). The average post-wash count was 1300/cu mm (range 200-3000). Fresh frozen plasma (62 units) and platelets (27 units) were given without special preparation. We followed 54 CMV-seronegative neonates who received WRC. Birth weights (BW) were less than 1500 gm in 43%,. Infants received 1-36 WRC transfusion (avg 6.0 per patient). These WRC were 51% seropositive. Serology (ELISA) and viral cultures were performed at an average of 89.6 days (range 18-147) following the last transfusion. Six infants developed laboratory evidence of CMV infection. Infections in 5 of the infants were asymptomatic (BW 1060, 1960, 2180, 2270 and 3500 gms). One infant (BW 720 gm) died after a very complicated course. Dissemination of CMV was noted at autopsy. Presently available methods of leukocyte depletion are inadequate to prevent PTCMV. Our data suggest that CMV-seronegative and frozen deglycerolized blood are preferable to WRC. However, the lack of symptoms in the infected infants suggests that WRC may offer an advantage over conventional blood products.
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Demmler, G., Brady, M., Bijou, H. et al. 1083 SALINE-WASHED RED BLOOD CELLS (WRC) UNSUCCESSFUL IN PREVENTING POST-TRANSFUSION CYTOMEGALOVIRUS INFECTION (PTCMV) IN NEONATES. Pediatr Res 19, 291 (1985). https://doi.org/10.1203/00006450-198504000-01113
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DOI: https://doi.org/10.1203/00006450-198504000-01113