Abstract
Transfusion acquired CMV infections should be prevented for seronegative (SN) premature infants by providing blood products from SN donors. In order to provide laboratories with a very simple and rapid method for detecting antibody against CMV, latex beads were coated with CMV antigen, incubated for 8 minutes at room temperature with 25ul of sera, and examined for agglutination. The sensitivity and specificity of LA was compared to indirect hemagglutination (IHA, Cetus) and enzyme immunoassay (EIA) using 316 sera from random blood donors. Of 177 sera SN by EIA and IHA, 177 had a LA titer of <1:4 (specificity=100%). Of 119 sera with detectable antibody by EIA and IHA, 115 had a LA titer of 1:4 or greater (sensitivity = 97%). Plasma (EDTA, heparin, and citrate) did not effect LA. LA was also used quantitatively to detect 4 fold or greater rises in antibody in paired sera from 10 patients with posttransfusion CMV infections. LA is a sensitive and specific assay that is extremely rapid and simple to perform.
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Adler, S., Marshall, D., McVoy, M. et al. 1046 THE DETECTION OF ANTIBODY AGAINST CYTOMEGALOVIRUS (CMV) USING LATEX AGGLUTINATION (LA). Pediatr Res 19, 285 (1985). https://doi.org/10.1203/00006450-198504000-01076
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DOI: https://doi.org/10.1203/00006450-198504000-01076