Abstract
Interleukin-1 (IL1) is a mononuclear phagocyte secretory product that modulates lymphocyte function, acute phase protein synthesis and hypothalamic thermal regulation. Cord blood monocytes (>90% esterase positive) were isolated from 28 infants ranging in gestation from 31 to 41 weeks, (mean 38.9, SD 2.58) and stimulated with 10 mcg/ml LPS (E.coli). Gontrol cultures contained medium alone or with 10 mcg/ml polymyxin B (PMB). Twenty-four hour supernatants were tested in a C3H/HeJ mouse thymocyte proliferation assay and mean response for 28 cord monocyte samples was 141422 cpm (SE 1499), similar to normal adults. Unstimulated monocytes from 16 of the 28 infants had ILl levels that increased proliferation to more than twice background (720 cpm), mean 5726 cpm (SE 1637). As PMB binds LPS, five PME controls were studied to investigate LPS contamination. Four of these controls had high unstimulated IL1 levels and two did not suppress significantly with PMB. The group with high unstimulated IL1 synthesis had significantly increased birth-weights (mean difference 0.50 kg, p<0.05), and longer gestations (mean difference 1.9 wks, p<0.05). Those with perinatal complications had significantly increased unstimulated activity (5897 vs 1655 cpm) and stimulated activity (15876 vs 12293 cpm) compared to normal deliveries. Thus 1) the IL1 response to LPS is intact in newborn human monocytes and 2) there is evidence of spontaneous activation of cord blood monocytes following complicated deliveries and in more mature pregnancies.
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Wilmott, R., Harris, M., Haines, K. et al. 1038 INTEELEUKIN-1 SYNTHESIS BY COED BLOOD MONOCYTES. Pediatr Res 19, 283 (1985). https://doi.org/10.1203/00006450-198504000-01068
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DOI: https://doi.org/10.1203/00006450-198504000-01068