Abstract
Ten positive antinuclear antibody (ANA) sera from JDMS patients obtained within 4 months of disease onset were examined by indirect immunofluorescence (IF), radial immunodiffusion, hemmaglutination, and enzyme immunoassays for the specificity of the Ab. Antihistone Ab was identified by IF using HEP2-HCl washed and H reconstituted slides. No sera had Ab to Sm, RNP, SS-A, SS-B, DNA, and Sc170. Seven of ten showed the presence of IgG, IgA-H Ab, and three IgM-H Ab. All patients with anti-H Ab were reconstituted with fraction H2b and H3 and three with H2a; after reconstitution, the initial IF homogeneous-speckled pattern changed to speckled-nucleolar in 5/7 H-positive sera. In vitro complement fixation with HEP2-H reconstituted slides and JDMS sera showed that Clq but not C3 and C4 fixed this immune complex.
We conclude that JDMS sera with positive ANA has more than one Ab and the H Ab may account for only a portion of the Abs, and immune complexes formed in the presence of JDMS-H sera fixed Clq only.
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Arroyave, C., Pachman, L., Rich, K. et al. 953 NUCLEOLAR ANTI-HISTONES (H) ANTIBODIES (Ab) INJUVENILE DERMATOMYOSITIS. Pediatr Res 19, 269 (1985). https://doi.org/10.1203/00006450-198504000-00983
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DOI: https://doi.org/10.1203/00006450-198504000-00983