Abstract
Increased respiratory complications have been reported in patients treated simultaneously with amphotericin-B (AB) and granulocyte (PMN) transfusions. Aggregation and sequestration of PMN's in the microvasculature of the lung has been postulated as a mechanism. This study was designed to see if AB could aggregate PMN's in vitro when the cells were not exposed to nylon wool fibers (NWF). Blood was obtained from 30 adults and PMN's were concentrated (10−7/ml) with Ficoll-Hypaque centrifugation and hypotonic erythrocyte lysis. Dose response curves were obtained in buffered saline (PBS). Aggregation did not occur with AB alone in the serum level range of 1-4 μg/ml, but was seen at AB concentrations greater than 12 μg/ml (change in light transmission over 1 min (ΔT) of greater than 2%). PMN's were then incubated with AB (2-4 μg/ml) in PBS or 2% albumin. Zymogen activated serum or FMLP were added when no aggregation occurred after 5 min. In PBS irreversible aggregation was seen, while in albumin normal aggregation-deaggregation pattern was seen (mean ΔT = 11%). The latter observations suggested that in vitro studies using PBS may not be comparable to the normal physiologic state with albumin and other serum proteins present. PMN's not exposed to NWF are aggregated in vitro by AB but only at higher concentrations than seen in vivo. Damage of PMN's during collection may be an important factor in enhanced PMN aggregation in lungs when amphotericin is used in conjunction with PMN transfusions.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olson, T., Cook, B., Burgess, D. et al. NEUTROPHIL AGGREGATION IN VITRO BY AMPHOTERICIN-B. Pediatr Res 18 (Suppl 4), 246 (1984). https://doi.org/10.1203/00006450-198404001-00916
Issue Date:
DOI: https://doi.org/10.1203/00006450-198404001-00916