Abstract
The in vivo effects of glycerol-3-phosphate (G3P) on gluconeogenesis from alanine (Ala) were investigated in well (C) and malnourished (M) rats. Sixty to 80 g Wistar rats were made M by feeding a 4% protein (Prt), 54% carbohydrate (Cho) diet for 4 weeks. C were fed a diet with 23% Prt and 63% Cho. Both groups received equal amounts of fat, vitamins and minerals. After an overnight fast the rats received a single i.p. dose of Ala (1 g/kg), with or without prior priming with 2 g/kg G3P, 30 min before Ala. Blood glucose (Glc) levels were monitored for 2 hr and noted as changes over baseline. The results indicate: (1) Both groups of rats produced Glc from Ala at the same rate (F=2.89; df=1,60; NS). (2) When G3P was injected before Ala, blood Glc declined in M rats to levels below baseline beginning 60 min after Ala administration (F=69.8; df=1,60; P<0.001, compared to Ala alone). C rats had no inhibition of Glc formation by G3P. Conclusions: in M rats, G3P administration inhibits normal gluconeogenesis from Ala, in contrast to C rats, in which both substrates show no interaction. Therefore, these data support the view that nutritional status may play a role in tolerance to glycerol, the metabolic precursor of G3P, especially early in life.
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Wapnir, R., Stiel, L. & Lifshitz, F. ALTERATIONS OF GLUCONEOGENESIS IN VIVO BY GLYCEROL-3-PHOSPHATE: EFFECTS OF EARLY MALNUTRITION. Pediatr Res 18 (Suppl 4), 302 (1984). https://doi.org/10.1203/00006450-198404001-01253
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DOI: https://doi.org/10.1203/00006450-198404001-01253