Abstract
Chemiluminescence (CL) is a luminol-dependent property of concanavalin A (con-A) stimulated, nylon wool purified T-lymphocytes. Peak stimulated response (CPM × 10−3 ± standard error) was 90±12, significantly (p<.001) greater than the background emission of 36±1.0. Utilizing linoleic acid (LA, 0.16 M) as both trigger and substrate in this luminol amplified system resulted in a mean peak response of 3327±1219 greater (p<.05)than the background of 41±2 and dramatically increased over the con-A induced response. Distinctive oxidative mechanisms are suggested not only by the nature of the stimulants in the reaction milieu, but altered kinetics (time to peak 2.4 minutes with con-A vs 25 with LA), differential sensitivity to anti-oxidant enzymes, and presence of the lipid peroxidative response alone in chronic granulomatous disease. We further explored lipid peroxidation in T-cells using an exquisitely sensitive luminometer (output range 0.01-10000 mV), eliminating the need for luminol. Peak and total luminescent responses (in mV) were measured in purified suspensions of T-cells and PMN:
The peroxidative potential of T-cells is further demonstrated by the superoxide dismutase resistant, LA stimulated reduction of ferricytochrome C (2.4 mM) , 17.85 nM reduced per 5.0 × 106 T-cells compared to the non-stimulated background of 3.95. LA is a uniquely potent, luminol-independent trigger of T-cell CL. This phenomenon may serve as a valuable probe of cellular activation.
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Peerless, A., Strom, S. & Stiehm, E. LINOLEIC ACID STIMULATION OF HUMAN T-LYMPHOCYTE CHEMILUMINESCENCE. Pediatr Res 18 (Suppl 4), 263 (1984). https://doi.org/10.1203/00006450-198404001-01019
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DOI: https://doi.org/10.1203/00006450-198404001-01019